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Direct and rapid detection of serum amino acid and monoamine neurotransmitters to assist the diagnosis of panic disorder

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Science China Chemistry 2025年第1期68卷 377-384页

作者： Shiwen Liu Xuerui Wan Meng Zhao Jiaqi Wang Weilan Wu Linlin You Yonggui Yuan Qian Xu Rong Gao Key Laboratory of Environmental Medicine Engineering Ministry of EducationSchool of Public HealthSoutheast UniversityNanjing 210009China Key Laboratory of Modern Toxicology of Ministry of Education Department of Hygienic Analysis and DetectionSchool of Public HealthNanjing Medical UniversityNanjing 211166China Department of Psychosomatics and Psychiatry Zhongda HospitalSchool of MedicineSoutheast UniversityNanjing 210009China

The measurement of amino acid and monoamine neurotransmitters(NTs)in serum plays a central role in the identification and monitoring of clinical panic disorder(PD).However,the strong polarity and chemical heterogeneity of NTs make direct extraction and rapid detection extremely ***,we developed a strategy that integrated solid phase extraction based on magnetic Fe_(3)O_(4)coated with polydopamine(Fe_(3)O_(4)@PDA)and direct analysis of mass spectrometry in real time(DART-MS)for quantification of 10 *** systematically investigated the optimal conditions of this strategy to achieve good accuracy and *** with existing magnetic solid phase extraction methods for NTs detection in biological samples,this strategy could adsorb more targets while having similar adsorption efficiencies,and the total process time was reduced by an average of 69.42%.We rapidly determined the metabolic abnormality of 6 types of NTs in the serum of PD patients and demonstrated their diagnostic *** addition,an outstanding feature of PD in which tryptophan was more metabolized by the KYN pathway and the 5-hydroxytryptamine pathway was inhibited could be elucidated by this *** results highlighted the potential ability of Fe_(3)O_(4)@PDA coupled with DART-MS to assist in the clinical diagnosis of PD.

关键词： neurotransmitters panic disorder magnetic solid phase extraction direct analysis of mass spectrometry in real time

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Determination of five neurotransmitters in the rat brain for the study of the hypnotic effects of Ziziphi Spinosae Semen aqueous extract on insomnia rat model by UPLC-MS/MS

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Chinese Journal of Natural Medicines 2019年第7期17卷 551-560页

作者： YAN Yan LI Qiang DU Hui-Zhi SHEN Chen-Xi LI Ai-Ping PEI Xiang-Ping DU Chen-Hui QIN Xue-Mei Modern Research Center for Traditional Chinese Medicine Shanxi University Taiyuan 030006 China School of Chinese Materia Medica Shanxi University of Traditional Chinese Medicine Taiyuan 030619 China

Ziziphi Spinosae Semen(ZSS) has been used for treatment of insomnia in China for centuries. To reveal the influence of insomnia on the levels of the neurotransmitters including serotonin(5-HT), glutamic acid(Glu),γ-aminobutyric acid(GABA),noradrenaline(NE) and dopamine(DA), and to study the role of ZSS aqueous extract in the treatment of insomnia, an UPLC-ESIMS/MS method was developed and validated for simultaneous determination of five neurotransmitters in the rat brain. The brain samples were pretreated by one-step direct protein precipitation with acetonitrile. The analytes were detected in positive mode with multiple reaction monitoring(MRM) and the procedure was completed in less than 10 min. The method showed a good linearity(R^2 >0.9967) with the other validation parameters were within acceptance range. The results indicated that the concentration of 5-HT, GABA and DA is significantly lower(P neurotransmitters in the brain.

关键词： Ziziphi Spinosae Semen neurotransmitters UPLC-MS/MS Hypnotic effects

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Neuro faces of beneficial T cells:essential in brain,impaired in aging and neurological diseases,and activated functionally by neurotransmitters and neuropeptides

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Neural Regeneration Research 2023年第6期18卷 1165-1178页

作者： Mia Levite Faculty of Medicine The Hebrew University of JerusalemCampus Ein KaremJerusalemIsrael Institute of Gene Therapy The Hadassah University Hospital-Ein KaremJerusalemIsrael

T cells are essential for a healthy life,performing continuously:immune surveillance,recognition,protection,activation,suppression,assistance,eradication,secretion,adhesion,migration,homing,communications,and additional *** paper describes five aspects of normal beneficial T cells in the healthy or diseased ***,normal beneficial T cells are essential for normal healthy brain functions:cognition,spatial learning,memory,adult neurogenesis,and neuroprotection.T cells decrease secondary neuronal degeneration,increase neuronal survival after central nervous system(CNS) injury,and limit CNS inflammation and damage upon injury and ***,while pathogenic T cells contribute to CNS disorders,recent studies,mostly in animal models,show that specific subpopulations of normal beneficial T cells have protective and regenerative effects in seve ral neuroinflammatory and neurodegenerative *** include M ultiple Sclerosis(MS),Alzheimer’s disease,Parkinson’s disease,Amyotrophic Lateral Sclerosis(ALS),stro ke,CNS trauma,chronic pain,and *** T cell-secreted molecules and direct cell-cell contacts deliver T cell neuroprotective,neuro regenerative and immunomodulato ry ***,normal beneficial T cells are abnormal,impaired,and dysfunctional in aging and multiple neurological *** T cell impairments are evident in aging,brain tumors(mainly Glioblastoma),seve re viral infections(including COVID-19),chro nic stress,major depression,schizophrenia,Parkinson’s disease,Alzheimer’s disease,ALS,MS,stro ke,and other *** main detrimental mechanisms that impair T cell function are activation-induced cell death,exhaustion,senescence,and impaired T cell *** urth,several physiological neurotransmitters and neuro peptides induce by themselves multiple direct,potent,beneficial,and therapeutically-relevant effects on normal human T cells,via their receptors in T *** scientific field is called "Nerve-Driven Im

关键词： aging dopamine glutamate nerve-driven immunity neurological diseases neuropeptides neurotransmitters Personalized Adoptive Neuro-Immunotherapy T cells

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Paper-Based Electrochemical Biosensors for Point-of-Care Testing of neurotransmitters

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Journal of Analysis and Testing 2019年第1期3卷 19-36页

作者： Yingchun Li Rongyan He Yan Niu Fei Li The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and TechnologyXi’an Jiaotong UniversityXi’an 710049People’s Republic of China Bioinspired Engineering and Biomechanics Center(BEBC) Xi’an Jiaotong UniversityXi’an 710049People’s Republic of China

Abstract neurotransmitters are important biological molecules related to several nervous system diseases(NSDs).Point-of-care test-ing(POCT)of neurotransmitters is of great importance in preclinical research and early diagnosis of *** various POCT platforms,paper-based electrochemical biosensors have achieved great advances in detection of neurotransmitters,thus taking a significant role in POCT of neurotransmitters *** review gives an overview of the recent advances of paper-based electrochemical biosensors for POCT of *** first introduce the types of neurotransmitters and biological sample sources mainly used for neurotransmitter ***,we review the components and the traditional fabrication technologies for paper-based electrochemical POCT biosensors,and then the functional modification methods of biosensor surfaces and three-dimensional fabrication methods for further enhancement of their detection ***,we list examples of paper-based electrochemical biosensors used for detecting different neurotransmitters in biologi-cal ***,we give a conclusion and promising development direction of paper-based electrochemical biosensors for neurotransmitters *** purpose of this review is to introduce the paper-based electrochemical biosensors as an emerging technology for POCT of neurotransmitters,offering a reference for readers and researchers for early diagnosis of NSDs using POCT technologies.

关键词： Point-of-care testing(POCT) Nervous system diseases(NSDs) neurotransmitters Paper-based electrochemical biosensors

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Correlation between cerebral neurotransmitters levels by proton magnetic resonance spectroscopy and HbA1c in patients with type 2 diabetes

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World Journal of Diabetes 2024年第6期15卷 1263-1271页

作者： Xiang-Yu Gao Chen-Xia Zhou Hong-Mei Li Min Cheng Da Chen Zi-Yi Li Bo Feng Jun Song Department of Endocrinology East HospitalTongji University School of MedicineShanghai 200120China Department of Endocrinology Qilu Hospital(Qingdao)Cheeloo College of MedicineShandong UniversityQingdao 266000Shandong ProvinceChina Department of Immunization Program Huangdao District Center for Disease Prevention and ControlQingdao 266400Shandong ProvinceChina

BACKGROUND Cognitive dysfunction is the main manifestation of central *** cognitive impairments tend to be overlooked in patients with diabetes mellitus(DM),there is a growing body of evidence linking DM to cognitive *** is closely related to neurological abnormalities,while often disregarded in clinical *** in cerebral neurotransmitter levels are associated with a variety of neurological abnormalities and may be closely related to blood glucose control in patients with type 2 DM(T2DM).AIM To evaluate the concentrations of cerebral neurotransmitters in T2DM patients exhibiting different hemoglobin A1c(HbA1c)*** A total of 130 T2DM patients were enrolled at the Department of Endocrinology of Shanghai East *** participants were divided into four groups according to their HbA1c levels using the interquartile method,namely Q1(neurotransmitters in the left hippocampus and left brainstem area were detected by proton magnetic resonance *** The HbA1c level was significantly associated with urinary microalbumin(mALB),triglyceride,low-density lipoprotein cholesterol(LDL-C),homeostasis model assessment of insulin resistance(HOMA-IR),and beta cell function(HOMA-β),N-acetylaspartate/creatine(NAA/Cr),and NAA/choline(NAA/Cho).Spearman correlation analysis showed that mALB,LDL-C,HOMA-IR and NAA/Cr in the left brainstem area were positively correlated with the level of HbA1c(P<0.05),whereas HOMA-βwas negatively correlated with the HbA1c level(P<0.05).Ordered multiple logistic regression analysis showed that NAA/Cho[Odds ratio(OR):1.608,95%confidence interval(95%CI):1.004-2.578,P<0.05],LDL-C(OR:1.627,95%CI:1.119-2.370,P<0.05),and HOMA-IR(OR:1.107,95%CI:1.031-

关键词： Type 2 diabetes mellitus Hemoglobin A1c Proton magnetic resonance spectroscopy neurotransmitters Central neuropathy

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EFFECTS OF MORPHINE AND MONOAMINE neurotransmitters ON PAIN MODULATION OF SUBSTANCE P IN THE SPINAL CORD

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Chinese Science Bulletin 1991年第8期36卷 682-686页

作者：阮怀珍李希成 Department of Physiology Third Military Medical College Chongqing 630038 PRC

Substance P (SP)in the spinal cord is unevenly distributed and is concentrated in the dorsal horn, particularly in terminals of unmyelinated nociceptive primary afferent fibers. It indicates that SP may be an excitatory neurotransmitter released from nociceptive primary afferent nerve terminals. It was reported that SP in the spinal cord took part both in

关键词： inmmnoreactive substance P and substance P-like immunoreactive fibers spinal cord morphine monoamine neurotransmitters pain threshold.

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Pseudotargeted metabolomics for exploring the changes of neurotransmitters profile in aging rat brain and the potential neuroprotective effect of alkaloids from Uncaria rhynchophylla

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Traditional Medicine Research 2022年第5期7卷 1-9页

作者： Shi-Wei Li Yi-Song Wu Shi-Fei Wu Wen-Long Wei Ming-Yang Qiu Yun Li De-An Guo College of Pharmacy Changchun University of Chinese MedicineChangchun 130117China Shanghai Research Center for Modernization of Traditional Chinese Medicine National Engineering Research Center of TCM Standardization TechnologyShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

Background:Aging is an essential risk factor for most neurodegenerative diseases,including Alzheimer’s disease and Parkinson’s ***,changes in the levels of neurotransmitters that are associated with aging are not well ***:Methods such as liquid-liquid extraction,protein precipitation,and solid-phase extraction,using 20 different extraction solvents,were evaluated to optimize the extraction of neurotransmitters.A pseudotargeted metabolomics approach was developed to detect neurotransmitters in brain tissues using ultra-high-performance liquid chromatography coupled with tandem triple quadrupole mass *** that crossed into the brain were used to evaluate the effect of glutamic acid-induced excitatory neurotoxicity in SH-SY5Y ***:The overall extraction efficiency using protein precipitation was *** changes in neurotransmitters’levels in the brain exhibited changes during the different growth *** levels of seven neurotransmitters(aspartic acid,tyrosine,isoleucine,leucine,tryptophan,valine,andγ-aminobutyric acid)were significantly ***,alkaloids could reduce the excitatory neurotoxicity of glutamic acid-induced SH-SY5Y cells via suppression of oxidative ***:Significant differences were observed in neurotransmitter profiling between 1-and 8-month-old rats,and the discrepant neurotransmitters were associated with *** indole alkaloids from Uncaria rhynchophylla,which could cross the blood-brain barrier,were screened and used to explore their protective effects against *** rhynchophylla alkaloids exhibited a neuroprotective effect by inhibiting oxidative stress,indicating that the alkaloid could be a potential therapeutic candidate for neurological disorders caused by glutamic acid toxicity.

关键词： neurotransmitters aging Uncaria rhynchophylla indole alkaloids neuroprotective effect pseudotargeted metabolomics

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Effects of Bushen Huoxue Formula on the Learning and Memory Function and the Cerebral neurotransmitters in Diabetic Mice

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Journal of Traditional Chinese Medicine 2010年第3期30卷 201-205页

作者：田国庆卢贺起郭赛珊张玲樊莎莎王友京 Peking Union Medical College Hospital Chinese Academy of Medical Sciences Institute of Chinese Medical Basic Theory China Academy of Chinese Medical Sciences College of Chinese Medicine Capital Medical University

Objective: To observe the effects of Bushen Huoxue Formula (Formula for reinforcing the kidney and activating blood circulation) on the learning and memory function and the cerebral neurotransmitters in diabetic mice. Methods: Forty ICR mice were randomized into the normal control group, model group, Nimotop group and Chinese medicine group, 10 mice in each group. Tail intravenous injection of alloxan was applied to prepare diabetic model. Four weeks later, intragastric administration of Bushen Huoxue Formula for the Chinese medicine group, Nimotop for the Nimotop group, and isometric distilled water for the other two groups were respectively given for 8 weeks. The changes in the blood sugar level were observed; the learning and memory function was detected by Morris labyrinth test; and the contents of norepinephrine (NE), dopamine (DA), 5-hydroxyltryptamine (5-HT) and 5-hydroxyl indole acetic acid (5-HIAA) in cerebral cortex were determined in mice of all the groups. Results: The blood sugar levels in the diabetic model mice significantly increased as compared with those of the normal control group determined 72 h and 12 weeks later (P<0.05 or P<0.01). Latencies for Morris labyrinth test in the Nimotop group and the Chinese medicine group were significantly shortened as compared with that in the model group (P<0.01). The contents of cortical NE in the Chinese medicine group was significantly higher than that in the model group (P<0.01). Conclusion: Bushen Huoxue Formula can improve the learning and memory function in the diabetic mice, and the mechanism is possibly related with change of the cortical NE content.

关键词： Bushen Huoxue Formula (Formula for reinforcing the kidney and activating blood circulation) diabetes learning and memory function neurotransmitters mice

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Protective Effect of Curcumin on Anxiety, Learning Behavior, Neuromuscular Activities, Brain neurotransmitters and Oxidative Stress Enzymes in Cadmium Intoxicated Mice

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Journal of Behavioral and Brain Science 2013年第1期3卷 74-84页

作者： Gasem M. Abu-Taweel Jamaan S. Ajarem Mohammad Ahmad Department of Biology College of Education Dammam University Dammam Saudi Arabia Department of Medical Surgical Nursing College of Nursing King Saud University Riyadh Saudi Arabia Department of Zoology College of Science King Saud University Riyadh Saudi Arabia

Cadmium (Cd) exposure can induce acute lethal health-related threats to humans since it has an exceptional ability to accumulate in living organisms and cause toxicological effects. Curcumin (Cur) on the other hand has a wide variety of biological activities and several animal studies have suggested for a potential therapeutic or preventive effects against several ailments and infections. To study the effect of Cur on the toxicity of Cd, sixty Swiss-Webster strain male mice were divided into 6 groups of ten each at random. Group-1 served as the na?ve control and received no treatment. Group-2, 3 and 4 were the experimental controls and were administered once a day with a single oral dose of 50% dimethyl sulphoxide (DMSO), Cur (300 mg/kg) or Cd (100 mg/kg) respectively, for 2 weeks. Group-5 and 6 received Cur and Cd in combination once a day orally for 2 weeks except that Cur in a dose of 150 and 300 mg/kg to group 5 and 6 respectively, was administered one hour before Cd (100 mg/kg) administration to both groups. After treatment period, the animals were subjected to behavioral tests and thereafter, the animals were sacrificed for the estimation of neurotransmitters like serotonin (5-HT), dopamine (DA) and it’s metabolite 3,4-dihydroxyphenylacetic acid (DOPAC) as well as oxidative stress enzymes like lipid peroxides in the form of thiobarbituric acid–reactive substances (TBARS) and total glutathione (GSH) in the forebrain tissue. Cd reduced significantly the body weight gain, the locomotor activity, anxiety behavior in the plus maze and the learning capability (cognitive effect) in the shuttle-box test. Biochemical analysis further revealed that Cd exposure significantly altered the brain neurotransmitters and the oxidative stress enzymes. However, administration of Cur along with Cd had an ameliorating effect on all the behavioral and biochemical parameters studied herein and reduced the toxicity of Cd significantly and dose-dependently. Thus, Cur may be beneficial

关键词： Curcumin Cadmium Male Mice Anxiety Cognitive Behaviors neurotransmitters Oxidative Stress

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Effects of a Combination of Fu Zi and Rou Gui on Intestinal neurotransmitters and Microflora in Rats with Slow Transit Constipation

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Agricultural Biotechnology 2023年第3期12卷 79-86,91页

作者： Yuchuan LI Yuanzhe ZHANG The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine Guiyang 550025China Guizhou University of Traditional Chinese Medicine Guiyang 550025China

[Objectives] This study was carried out to explore the combined effects of Fu Zi(Radix Aconiti Lateralis Praeparata, the secondary root of perennial herbaceous plant Acontium carmichaeli Dehx. of family Ranunculaceae) and Rou Gui(Cortex Cinnamomi, the bark of Cinnamamunz cassia Presl of family Lauraceae) on intestinal neurotransmitters and microflora in rats with slow transit constipation(STC). [Methods] Experimental rats were given loperamide hydrochloride by gavage to induce STC, and then treated with Fu Zi alone, Rou Gui alone, a combination of Fu Zi and Rou Gui(2:1 w/w), and prucalopride, respectively, for 14 days. Meanwhile, the general condition, the time to first black stool and the rate of intestinal propulsion of rats in each group were observed after STC was induced and after drug treatment, and the pathological changes in rat colon were observed via hematoxylin-eosin(HE) staining, and the levels of colonic 5-hydroxytryptamine(HT), vasoactive intestinal peptide(VIP) and substance P(SP) were detected by ELISA, and the changes in intestinal flora were detected by 16S rRNA Real-time PCR. [Results] Compared with healthy rats, the time to first black stool and the rate of intestinal propulsion, colonic 5-HT and SP levels significantly decreased(p<0.01), while their colonic VIP level significantly increased(p<0.01). Compared with STC rats, the time to first black stool, the rate of intestinal propulsion, colonic 5-HT and SP levels in Fu Zi-Rou Gui(2:1) treated rats and prucalopride treated rats significantly increased(p<0.01), while their colonic VIP level significantly decreased(p<0.01). There was no significant difference in alpha diversity between healthy rats and STC rats. However, analysis on beta diversity revealed that there were differences in microflora structure and composition between them. Compared with healthy rats, the relative abundance of Firmicutes and Proteobacteria in STC rats significantly increased, while that of Bacteroidetes decreased. Com

关键词： Slow transit constipation(STC) Fu Zi-Rou Gui Intestinal motility neurotransmitters Intestinal microflora

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