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Microelectrode Array-evaluation of Neurotoxic Effects of Magnesium as an Implantable Biomaterial

Journal of Materials Science & Technology 2016年第1期32卷 89-96页

作者： Ting Huang ZhonghaiWang Lina Wei Mark Kindy Yufeng Zheng Tingfei Xi Bruce Z. Gao Center for Biomedical Materials and Tissue Engineering Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China Department of Bioengineering Clemson University Clemson SC29634 USA Departments of Neurosciences and Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston SC29466 USA Ralph H. Johnson VA Medical Center Charleston SC29403 USA Department of Materials Science and Engineering College of Engineering Peking University Beijing 100871 China Shenzhen Key Laboratory of Human Tissue Regeneration and Repair Shenzhen Institute Peking University Shenzhen 518057 China

Magnesium （Mg）-based biomaterials have shown great potential in clinical applications. However, the cytotoxic effects of excessive Mg2. and the corrosion products from Mg-based biomaterials, particularly their effects on neurons, have been little studied. Although viability tests are most commonly used, a functional evaluation is critically needed. Here, both methyl thiazolyl tetrazolium （MTT） and lactate de- hydrogenase （LDH） assays were used to test the effect of Mg2. and Mg-extract solution on neuronal viability. Microelectrode arrays （MEAs）, which provide long-term, real-time recording of extracellular electro- physiological signals of in vitro neuronal networks, were used to test for toxic effects. The minimum effective concentrations （ECmin） of Mg2. from the MTr and LDH assays were 3 mmol/L and 100 mmol/L respec- tively, while the ECmin obtained from the MEA assay was 0.1 mmol/L MEA data revealed significant loss of neuronal network activity when the culture was exposed to 25% Mg-extract solution, a concentra- tion that did not affect neuronal viability. For evaluating the biocompatibility of Mg-based biomaterials with neurons, MEA electrophysiological testing is a more precise method than basic cell-viability testing.

关键词： Magnesium Microelectrode array neuroelectrophysiology Neuron viability Neuronal network

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Nanoparticles carrying neurotrophin-3-modified Schwann cells promote repair of sciatic nerve defects

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Neural Regeneration Research 2013年第14期8卷 1262-1268页

作者： Haibin Zong Hongxing Zhao Yilei Zhao Jingling Jia Libin Yang Chao Ma Yang Zhang Yuzhen Dong Functional Laboratory School of Basic Medical SciencesXinxiang Medical College Department of Orthopedics the First Affiliated Hospital of Xinxiang Medical College

Schwann ceils and neurotrophin-3 play an important role in neural regeneration, but the secretion of neurotrophin-3 from Schwann cells is limited, and exogenous neurotrophin-3 is inactived easily in vivo. In this study, we have transfected neurotrophin-3 into Schwann cells cultured in vitro using nanoparticle liposomes. Results showed that neurotrophin-3 was successfully transfected into Schwann cells, where it was expressed effectively and steadily. A composite of Schwann cells transfected with neurotrophin-3 and poly（lactic-co-glycolic acid） biodegradable conduits was transplanted into rats to repair 10-mm sciatic nerve defects. Transplantation of the composite scaffold could restore the myoelectricity and wave amplitude of the sciatic nerve by electrophysiological examination, promote nerve axonal and myelin regeneration, and delay apoptosis of spinal motor neurons. Experimental findings indicate that neurotrophin-3 transfected Schwann cells combined with bridge grafting can promote neural regeneration and functional recovery after nerve injury.

关键词： neural regeneration peripheral nerve injury neurotrophin-3 nanoparticle liposome Schwanncells sciatic nerve neuroelectrophysiology gene transfection grants-supported paper neuroregeneration

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A rat pup model of cerebral palsy induced by prenatal inflammation and hypoxia

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Neural Regeneration Research 2013年第9期8卷 817-824页

作者： Yanrong Hu Gang Chen Hong Wan Zhiyou Zhang Hong Zhi Wei Liu Xinwei Qian Mingzhao Chen Linbao Wen Feng Gao Jianxin Li Lihui Zhao Postdoctoral Research Station School of Basic Medicine CAMA and PUMC Postdoctoral Research Station the People's Hospital of Xinjiang Uygur Autonomous Region Department of Neurosurgery the Fourth People's Hospital of Wuxi (The Fourth Affiliated Hospital of Soochow University) Beijing Neurosurgical Institute Department of Neurosurgery Xinjiang Autonomous Region People's Hospital Department of Neurosurgery Xinjiang Autonomous Region People's Hospital Department of Neurology Xinjiang Autonomous Region People's Hospital Department of Pathology Xinjiang Autonomous Region People's Hospital

Animal models of cerebral palsy established by simple infection or the hypoxia/ischemia method cannot effectively simulate the brain injury of a premature infant. Healthy 17-day-pregnant Wistar rats were intraperitoneally injected with lipopolysaccharide then subjected to hypoxia. The pups were used for this study at 4 weeks of age. Simultaneously, a hypoxia/ischemia group and a control group were used for comparison. The results of the footprint test, the balance beam test, the water maze test, neuroelectrophysiological examination and neuropathological examination demonstrated that, at 4 weeks after birth, footprint repeat space became larger between the forelimbs and hindlimbs of the rats, the latency period on the balance beam and in the Morris water maze was longer, place navigation and ability were poorer, and the stimulus intensity that induced the maximal wave amplitude of the compound muscle action potential was greater in the lipopolysaccharide/hypoxia and hypoxia/ischemia groups than in the control group. We observed irregular cells around the periventricular area, periventricular leukomalacia and breakage of the nuclear membrane in the lipopolysacchadde/hypexia and hypoxia/ischemia groups. These results indicate that we successfully established a Wistar rat pup model of cerebral palsy by intraperitoneal injection of lipopolysaccharide and hypoxia.

关键词： neural regeneration brain injury hypoxia lipopolysaccharide animal models cerebral palsy watermaze test neuroelectrophysiology histopathology grants-supported paper photographs-containing paper neuroregeneration

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The sensitivity difference between the glans penis and penile shaft in primary premature ejaculation

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Asian Journal of Andrology 2023年第4期25卷 487-491页

作者： Lei Zheng Le-Tian Wei Qi-Zhen Tang Chun-Li Song Wen-Rong Liu Ke-Nan Wang Hui Jiang Tao Jiang Department of Andrology Second Hospital of Dalian Medical UniversityDalian 116000China Department of Urology First Hospital of Dalian Medical UniversityDalian 116000China Department of neuroelectrophysiology First Hospital of Dalian Medical UniversityDalian 116000China Department of Andrology Peking University First HospitalBeijing 100191China.

The penis is a vital organ of perception that transmits perceived signals to ejaculation-related *** penis consists of the glans penis and penile shaft,which differ considerably in both histology and *** paper aims to investigate whether the glans penis or the penile shaft is the main source of sensory signals from the penis and whether penile hypersensitivity affects the whole organ or only part of *** thresholds,latencies,and amplitudes of somatosensory evoked potentials(SSEPs)were recorded in 290 individuals with primary premature ejaculation using the glans penis and penile shaft as the sensory *** thresholds,latencies,and amplitudes of SSEPs from the glans penis and penile shaft in patients were significantly different(all P<0.0001).The latency of the glans penis or penile shaft was shorter than average(indicating hypersensitivity)in 141(48.6%)cases,of which 50(35.5%)cases were sensitive in both the glans penis and penile shaft,14(9.9%)cases were sensitive in the glans penis only,and 77(54.6%)cases were sensitive in the penile shaft only(P<0.0001).There are statistical differences in the signals perceived through the glans penis and the penile *** hypersensitivity does not necessarily mean that the whole penis is *** classify penile hypersensitivity into three categories,namely,glans penis,penile shaft,and whole-penis hypersensitivity,and we propose the new concept of penile hypersensitive zone.

关键词： neuroelectrophysiology perceptive zone premature ejaculation sensitivity sensory perception

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Early cyclosporin A treatment retards axonal degeneration in an experimental peripheral nerve injection injury model

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Neural Regeneration Research 2015年第2期10卷 266-270页

作者： Ibrahim Erkutlu Mehmet Alptekin Sirma Geyik Abidin Murat Geyik Inan Gezgin Abdulvahap G?k Department of Neurosurgery Faculty of Medicine University of Gaziantep Department of Neurology Faculty of Medicine University of Gaziantep Department of Neurosurgery Sanko University Department of Neurosurgery Private Park Hospital

Injury to peripheral nerves during injections of therapeutic agents such as penicillin G potas-sium is common in developing countries. It has been shown that cyclosporin A, a powerful immunosuppressive agent, can retard Wallerian degeneration after peripheral nerve crush injury. However, few studies are reported on the effects of cyclosporin A on peripheral nerve drug in-jection injury. This study aimed to assess the time-dependent efifcacy of cyclosporine-A as an immunosuppressant therapy in an experimental rat nerve injection injury model established by penicillin G potassium injection. The rats were randomly divided into three groups based on the length of time after nerve injury induced by cyclosporine-A administration （30 minutes, 8 or 24 hours）. The compound muscle action potentials were recorded pre-injury, early post-injury （within 1 hour） and 4 weeks after injury and compared statistically. Tissue samples were taken from each animal for histological analysis. Compared to the control group, a significant im-provement of the compound muscle action potential amplitude value was observed only when cyclosporine-A was administered within 30 minutes of the injection injury （P 〈 0.05）; at 8 or 24 hours after cyclosporine-A administration, compound muscle action potential amplitude was not changed compared with the control group. Thus, early immunosuppressant drug therapy may be a good alternative neuroprotective therapy option in experimental nerve injection injury induced by penicillin G potassium injection.

关键词： nerve regeneration peripheral nerve injury injection injury cyclosporine A penicillin G potassium Wallerian degeneration neuroelectrophysiology neural regeneration

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Research progress in hepatic encephalopathy in recent 10 years A Web of Science-based literature analysis

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Neural Regeneration Research 2012年第34期7卷 2727-2733页

作者： Nan Li Department of Digestion the 309 Hospital of Chinese PLA

OBJECTIVE： To analyze international research trends in hepatic encephalopathy and examine the role of neuroelectrophysiology and neuroimaging in diagnosis of hepatic encephalopathy. DATA RETRIEVAL： We performed a bibliometric analysis of studies on hepatic encephalopathy published during 2002-2011 retrieved from Web of Science. SELECTION CRITERIA： Inclusion criteria：（1） peer-reviewed published articles on hepatic encephalopathy; （2） original article, review, meeting abstract, proceedings paper, book chapter, editorial material, news items, and （3） published during 2002-2011. Exclusion criteria：（1） articles that required manual searching or telephone access; （2） documents that were not published in the public domain; and （3） corrected papers from the total number of articles. MAIN OUTCOME MEASURES：（1）Annual publication output; （2） type of publication; （3） publication by research field; （4） publication by journal; （5） publication by author; （6） publication by institution; （7） publication by country; （8） publication by institution in China; （9） most-cited papers. RESULTS： A total of 3 233 papers regarding hepatic encephalopathy were retrieved during 2002-2011. The number of papers gradually increased over the 10-year study period and was highest in 2010. Most papers appeared in journals with a focus on gastroenterology and hepatology. Among the included journals, Hepatology published the greatest number of papers regarding hepatic encephalopathy, and the published studies were highly cited. Thus, Hepatology appears to represent a key journal publishing papers on hepatic encephalopathy. Regarding distribution by country for publications on hepatic encephalopathy indexed in Web of Science during 2002-2011, the United States published highest number of papers, with China ranked ninth As per distribution by institute for publications, the University of Montreal in Canada published the highest number of papers （n = 111 ）. Among the Chinese institutes, Zhejiang University

关键词： hepatic encephalopathy neuroelectrophysiology neuroimaging diagnosis ammonia manganese astrocyte amino acids unbalance bibliometrics neural regeneration

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Multi-scale phase average waveform of electroencephalogram signals in childhood absence epilepsy using wavelet transformation

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Neural Regeneration Research 2010年第10期5卷 774-780页

作者： Meiyun Zhang Benshu Zhang Fenglou Wang Ying Chen Nan Jiang Department of Neurology General Hospital of Tianjin Medical University Tianjin 300052 China Department of Neurology Tianjin People's Hospital Tianjin 300130 China Department of Mechanics Tianjin University Tianjin 300051 China

BACKGROUND： Recent studies have focused on various methods of wavelet transformation for electroencephalogram （EEG） signals. However, there are very few studies reporting characteristics of multi-scale phase waves during epileptic ***： To extract multi-scale phase average waveforms from childhood absence epilepsy EEG signals between time and frequency domains using wavelet transformation, and to compare EEG signals of absence seizure with pre-epileptic seizure and normal children, and to quantify multi-scale phase average waveforms from childhood absence epilepsy EEG signals. DESIGN, TIME AND SETTING： The case-comparative experiment was performed at the Department of neuroelectrophysiology, Tianjin Medical University from August 2002 to May 2005. PARTICIPANTS： A total of 15 patients with childhood absence epilepsy from the General Hospital of Tianjin Medical University were enrolled in the study. The patients were not administered anti-epileptic drugs or sedatives prior to EEG testing. In addition, 12 healthy, age- and gender-matched children were also ***： EEG signals were tested on 15 patients with childhood absence epilepsy and 12 normal children. Epileptic discharge signals during clinical and subclinical seizures were collected 10 and 20 times, respectively. The collected EEG signals were treated with wavelet transformation to extract multi-scale characteristics during absence epilepsy seizure using a conditional sampling method. Multi-scale phase average waveforms were collected using a conditional phase averaging technique. Amplitude of phase average waveform from EEG signals of epilepsy seizure, subclinical epileptic discharge, and EEG signals of normal children were compared and statistically analyzed in the first *** OUTCOME MEASURES： Multi-scale wavelet coefficient and the evolution of EEG signals were observed during childhood absence epilepsy seizures using wavelet transformation. Multi-scale phase average wavefo

关键词： EEG multi-scale absence epilepsy wavelet transform phase average waveform neuroelectrophysiology neural regeneration

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Blockage of the habenular nucleus can eliminate dyspnea induced by electrostimulation of the insular cortex

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Neural Regeneration Research 2010年第13期5卷 1025-1029页

作者： Mingxian Li Jinghua Wang Min Huang Weihong Lin Min Wang Lei Yu Shao Wang Department of Respiratory Medicine First Hospital Jilin University Changchun 130021 Jilin Province China Department of Physiology Bethune School of Medicine Jilin University Changchun 130021 Jilin Province China Department of Neurology First Hospital Jilin University Changchun 130021 Jifin Province China

BACKGROUND： The insular cortex and habenular nucleus may be a regulatory center for obstructive sleep apnea syndrome, and dyspnea may be caused by insular cortex activity. The insular cortex is a cortical representation of obstructive sleep apnea syndrome. The habenular nucleus is a station for descending insular cortex activity. OBJECTIVE： Through actively stimulating the rat insular cortex, to observe rat respiratory movement, myoelectric activities of genioglossus, arterial partial pressure of oxygen, partial pressure of carbon dioxide and acidity-alkalinity, and to verify a hypothesis that the insular cortex is a superior-position regulation center, and the habenular nucleus is an inferior-position nervous nuclei of the insular cortex in patients with obstructive sleep apnea syndrome. DESIGN, TIME AND SETTING： The randomized, controlled animal study was performed at the Laboratory of Electrophysiology, Department of Physiology, Norman Bathune College of Medicine, Jilin University, China from September 2004 to June 2008. MATERIALS： We used L-glutamic acid （Dingguo Biological Product Research Center, Beijing, China）, lidocaine hydrochloride （Seventh Pharmacy Co., Ltd., Wuxi, China）, electric stimulator （Nihon Kohden, Japan）, and an AVL-OPTI blood gas analyzer （AVL Scientific Co., Roswell, GA, USA）. METHODS： The insular cortex of healthy adult Wistar rats underwent electrostimulation and L-glutamic acid stimulation to record changes in the myoelectric activity of genioglossus and respiratory movement. Some rats were injected with lidocaine to block the habenular nucleus before electrostimulation or L-glutamic acid stimulation. L-glutamic acid and lidocaine were injected by microelectrodes embedded in nuclear groups. MAIN OUTCOME MEASURES： Myoelectric activities of genioglossus, arterial partial pressure of oxygen, partial pressure of carbon dioxide and acidity-alkalinity were measured following apnea in rats undergoing electrostimulation in the insular cortex and f

关键词： dyspnea habenular nucleus insular cortex genioglossus neuroelectrophysiology neural regeneration

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A　CLINICAL　AND　NEUROELECTROPHYSIOLOGICAL　STUDY　OF　HYPERKALEMIC　PERIODIC　PARALYSIS

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Chinese Medical Sciences Journal 1995年第2期10卷 116-118页

作者：高秀贤汤晓芙杜华李本红 PUMC Hospital Beijing.

A case of atypical hyperkalemic periodic paralysis is reported. The diagnosis was confirmed by hyperkalemic test, cold water test, and differentiation of attack period and rest period by the measurement of motor nerve... 详细信息

关键词： hyperkalemic periodic paralysis neuroelectrophysiology

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STUDY OF BEHAVIORAL TOXICOLOGY IN LEAD EXPOSED RATS

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Journal of Pharmaceutical Analysis 1998年第2期12卷 141-145页

作者：张进陈等周明 Department of Occupational Health College of public health Xi'an Medical University Xi'an 710061 China

The study treated 72 s,rague-Dawley rats that were divided into 4 groups, one controlgroup and low, middle and higa dose groups, through drinking lead acetate solutions for threemonths. On the basis of founding subclinical lead poisoning model, behavioral toxicological testwhich consisted of neurobehavioral functions, neuroelectrophysiology and neurobiochemistry wascarried out. The results indicabo that low lvel lead exposure could result in the obvious changes orneurobehavioral function, neuroelectrophyslology and ueurobiochemistry, and the changes of neurobeltavioral runctiou had close correlatiom with P6B, Zap, NCV and DA, and they also had promlnant dose-response relatiouskips. The results suggested that the indexes of neurobehavioral functionmight be cousldered as early, semitive indexes for subolinical'lead poisoning. The combination ofneurobehavioral function with neuroectrophysiology could be used to evaluate the early neurotic toxicity of lead. The results also suggested that the change of dopamine metabolism of the central nervous system (CNS) might be one of the biological foundation of lead neurotic toxicity which changedthe neurobehavioral function of laboratory rats.

关键词： lead poisoning behavioral toxicology neurobehavioral function neuroelectrophysiology neurobiochemistry

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