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The Salicylic Acid Receptor NPR3 Is a negative regulator of the Transcriptional Defense Response during Early Flower Development in Arabidopsis

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Molecular Plant 2013年第3期6卷 802-816页

作者： Zi Shi Siela Maximova Yi Liu Joseph Verica Mark J. Guiltinan The Huck Institutes of the Life Sciences Pennsylvania State University University Park PA 16802 USA Department of Plant Science Pennsylvania State University University Park PA 16802 USA

Arabidopsis NON-EXPRESSOR OF PR1 （NPR1） is a transcription co-activator that plays a central role in regulating the transcriptional response to plant pathogens. The NPR family consists of NPR1 and five NPRl-like genes. The NPR1 paralog NPR3 has recently been shown to function as a receptor of the plant hormone salicylic acid and to mediate proteosomal degradation of NPR1. The function of NPR3 protein during early flower development was revealed through a detailed molecular-genetic analysis including promoter transcriptional fusion analysis, phenotype characterization of npr3-3 mutants/overexpressors, and whole-plant fitness analysis. The physical interaction between NPR3 and NPR1/ TGA2 was explored using bimolecular fluorescence complementation analysis in onion epidermal cells, Here, we show that NPR3 expression was strongest in the petals and sepals of developing flowers and declined after flower opening. Consistently with this observation, an npr3 knockout mutant displayed enhanced resistance to Pseudomonas syringae infection of immature flowers, but not leaves. Developing npr3 flowers exhibited increased levels of basal and induced PR1 transcript accumulation. However, the npr3 mutant showed lower fitness compared to Col-0 in the absence of pathogen. Moreover, NPR3 was shown to interact with NPR1 and TGA2 in vivo. Our data suggest that NPR3 is a negative regulator of defense responses during early flower development and it may function through the association with both NPR1 and TGA2.

关键词： plant defense NPR3 negative regulator Arabidopsis flower.

A Dual-Function Transcription Factor, AtYY1, Is a Novel negative regulator of the Arabidopsis ABA Response Network

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Molecular Plant 2016年第5期9卷 650-661页

作者： Tian Li Xiu-Yun Wu Hui Li Jian-Hui Song Jin-Yuan Liu Laboratory of Plant Molecular Biology Centre for Plant Biology School of Life Sciences Tsinghua University Beijing 100084 China Key Laboratory of Crop Gene Resources and Germplasm Enhancement Ministry of Agriculture Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 100081 China Department of Plant Pathology China Agricultural University Beijing 100193 China

Abscisic acid （ABA） plays crucial roles in plant growth and development, as well as in response to various environmental stresses. To date, many regulatory genes involved in the ABA response network have been identified; however, their roles have remained to be fully elucidated. In this study, we iden- tified AtYY1, an Arabidopsis homolog of the mammalian C2H2 zinc-finger transcription factor Yin Yang 1 （YY1）, as a novel negative regulator of the ABA response. AtYY1 is a dual-function transcription factor with both repression and activation domains. The expression of AtYY1 was induced by ABA and stress conditions including high salt and dehydration. The yyl mutant was more sensitive to ABA and NaCI than the wild-type, while overexpressing AtYY1 plants were less sensitive. AtYY1 loss also enhanced ABA-induced stomatal closing and drought resistance. Moreover, AtYYI can bind the ABA REPRESSOR1 （ABR1） promoter and directly upregulate ABR1 expression, as well as negatively regulate ABA- and saR-responsive gene expression. Additional analysis indicated that ABA INSENSITIVE4 （ABI4） might positively regulate AtYY1 expression and that ABR1 can antagonize this regulation. Our findings provide direct evidence that AtYY1 is a novel negative regulator of the ABA response network and that the ABI4-AtYY1-ABR1 regulatory pathway may fine-tune ABA-responsive gene expression in Arabidopsis.

关键词： AtYY1 ABA response negative regulator ABR1 ABI4

CPC, a Single-Repeat R3 MYB, Is a negative regulator of Anthocyanin Biosynthesis in Arabidopsis

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Molecular Plant 2009年第4期2卷 790-802页

作者： Hui-Fen Zhu Karen Fitzsimmons Abha Khandelwal Robert G. Kranz Department of Biology Washington University St Louis MO 63130 USA

Single-repeat R3 MYB transcription factors like CPC （CAPRICE） are known to play roles in developmental processes such as root hair differentiation and trichome initiation. However, none of the six Arabidopsis single-repeat R3 MYB members has been reported to regulate flavonoid biosynthesis. We show here that CPC is a negative regulator of anthocyanin biosynthesis. In the process of using CPC to test GAL4-dependent driver lines, we observed a repression of anthocyanin synthesis upon GAL4-mediated CPC overexpression. We demonstrated that this is not due to an increase in nutrient uptake because of more root hairs. Rather, CPC expression level tightly controls anthocyanin accumulation. Microarray analysis on the whole genome showed that, of 37 000 features tested, 85 genes are repressed greater than three-fold by CPC overexpression. Of these 85, seven are late anthocyanin biosynthesis genes. Also, anthocyanin synthesis genes were shown to be down-regulated in 35S：：CPC overexpression plants. Transient expression results suggest that CPC competes with the R2R3-MYB transcription factor PAP1/2, which is an activator of anthocyanin biosynthesis genes. This report adds anthocyanin biosynthesis to the set of programs that are under CPC control, indicating that this regulator is not only for developmental programs （e.g. root hairs, trichomes）, but can influence anthocyanin pigment synthesis.

关键词： CPC (CAPRICE) anthocyanin biosynthesis negative regulator Arabidopsis.

IRF10 is a negative regulator of IFN transcription in zebrafish

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IRF10 is a negative regulator of IFN transcription in zebraf...

中国水产学会鱼病专业委员会2013年学术研讨会

作者： Shun Li Hong Feng Nan Wu Jun Zou Yibing Zhang Yong-An Zhang Institute of Hydrobiology Chinese Academy of Sciences School of Biological Sciences University of Aberdeen

Interferon regulatory factor(IRF)10 belongs to IRF family and exists exclusively inbird and *** IRFs have been identified as critical regulators in interferon(IFN)responseboth in fish and mammals;however,the role of IRF10 is still *** this study,we identifiedIRF10 as a novel IRF member by which IFN activation was negatively regulated to balance theinnate immune reaction in zebrafish,Danio ***,Real-time PCR analysis indicated thatzebrafish IRF10(DrIRF10)was induced by intracellular poly I:C in ZF4(zebrafish embryofibroblast-like)cells,suggesting that DrIRF10 is likely a typical IFN-stimulated gene(ISG).Further reporter assay demonstrated that the activations of zebrafish IFN1 and IFN3(DrIFN1 andDrIFN3)promoters can be inhibited by DrIRF10 in the presence or absence of poly I:Cstimulation in EPC(Epithelioma papulosum cyprinid)cells,and such inhibition was also observedon the activation of IFN stimulatory response elements(ISREs).Meanwhile,overexpression ofDrIRF10 was also able to abolish the induction of DrIFN1 and DrIFN3 by the pivotal moleculesof Retinoic acid-inducible gene I(RIG-I)-like receptor(RLR)signaling ***,functional domain analysis of DrIRF10 showed that the DNA binding domain(DBD),rather thanIRF association domain(IAD),is essential for its inhibitory activity against IFN ***,overexpression of DrIRF10 decreased the transcription level of several IFN-stimulated genes,resulting in the vulnerability of host cells to spring viremia of carp virus(SVCV)***,these data suggest that DrIRF10 inhibits the expression of DrIFN1 and DrIFN3 viaits DBD domain to avoid excessive immune reaction,which reveals a distinguished regulationmechanism of IFN response in lower vertebrates.

关键词： IRF10 negative regulator IFN zebrafish

HCV core protein enhancing negative regulator A20 expression and relevant mechanism study in macrophages

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HCV core protein enhancing negative regulator A20 expression...

第十届全国免疫学学术大会

作者： Xiaotian Song Zhiyan Yao Jianling Yang Zhengzheng Zhang Lin Wei Hebei Medical University

Aim:Hepatitis C virus(HCV)infection is characterized by a strong propensity toward *** chronic HCV infection,HCV core protein is implicated in dysregulating innate immune *** we explore the effects of HCV core protein on macrophage and the potential signal pathways ***:Monocyte-derived human macrophage line(MΦ-THP-1)was treated with HCV core *** level of cytokines were determined by CBA and the changes of intracellular proteins were analyzed by Western *** packaged short hairpin RNA were used to silencing A20 and g C1q R ***:we demonstrated that HCV core protein induced macrophage A20 expression and IL-6,IL-1βand TGF-β1secretion.A20 is a powerful inhibitor of cytokine *** A20 dramatically enhanced the production of these ***,Pull-down assay showed that HCV core protein could interact with g C1q R but not TLR2 and TLR4,and silencing g C1q R abrogated HCV core-induced A20 high expression,which suggested that HCV core protein promoted A20 expression in macrophage via combining with g C1q ***,we found that HCV core protein could induce phosphorylation of p38,JNK,ERK,NF-κB p65 and NF-κB p105 in *** inhibition of p38,JNK and NF-κB but not ERK activity significantly reduced A20 ***:These results suggest that HCV core/g C1q R ligation induces A20 expression in macrophage via p38,JNK and NF-κB pathways,which may lead to a low-grade chronic inflammation condition in *** represents a novel mechanism by which HCV usurps the host for persistence.

关键词： HCV core Macrophage g C1q R negative regulator A20

NRL3 Interacts with OsK4 to Regulate Heading Date in Rice

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Rice science 2022年第3期29卷 237-246页

作者： CHEN Wei CAI Yicong Shakeel AHMAD WANG Yakun AN Ruihu TANG Shengjia GUO Naihui WEI Xiangjin TANG Shaoqing SHAO Gaoneng JIAO Guiai XIE Lihong HU Shikai SHENG Zhonghua HU Peisong Key Laboratory of Crop Physiology Ecology and Genetic BreedingMinistry of Education/Collaboration Center for Double-Season Rice Modernization ProductionJiangxi Agricultural University/Research Center of Super Rice Engineering and TechnologyJiangxi ProvinceNanchang 330045China State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding Ministry of Agriculture and Rural Affairs/China National Rice Improvement Center/China National Rice Research InstituteHangzhou 310006China

NRL3 is essential for the growth and development of rice *** this study,we found that the loss function of NRL3 also delayed heading date under natural long daylight and short daylight *** yeast two-hybrid and the bimolecular fluorescence complementation proved that NRL3interacts with OsK4,a Snf1-related ***4 localized to the nucleus and expressed in various rice *** rhythmic expression pattern of Os K4 was similar to NRL3 under long daylight and short daylight ***-out mutants of Os K4 exhibited early heading under long daylight conditions,indicating that it acts as a negative regulator of heading date in ***,the OsK4 mutant under the nrl3 mutant background rescued the late heading phenotype of nrl3 under long daylight conditions,suggesting that Os K4 functions downstream of ***,both NRL3 and Os K4 controlled heading date through regulating the expression of Hd3a and RFT1 *** findings shed light on the heading date regulation in rice and provide a sound theoretical base to improve regional adaptability of rice.

关键词： Oryza sativa heading date NRL3 gene OsK4 gene negative regulator loss function

Cold signaling in plants:Insights into mechanisms and regulation

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Journal of Integrative Plant Biology 2018年第9期60卷 745-756页

作者： Xiaoyu Guo Dongfeng Liu Kang Chong Key Laboratory of Plant Molecular Physiology Institute of Botanythe Chinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China

To survive under cold temperatures plants must be able to perceive a cold signal and transduce it into downstream components that induce appropriate defense mechanisms. In addition to inducing adaptive defenses, such as the production of osmotic factors to prevent freezing and the reprogramming of transcriptional pathways, cold temperatures induce changes in plant growth and development which can affect the plant life cycle. In this review, we summarize recent progress in characterizing cold-related genes and the pathways that allow transduction of the cold signal in plants, focusing primarily on studies in Arabidopsis tbaliana and rice （Oryza sativa）. We summarize cold perception and signal transduction from the plasma membrane to the nucleus, which involves cold sensors, calcium signals, calciumbinding proteins, mitogen-activated protein kinase cascades, and the C-repeat binding factor/dehydrationresponsive element binding pathways, as well as trehalose metabolism. Finally, we describe the balance between plant organogenesis and cold tolerance mechanisms in rice. This review encapsulates the known cold signaling factors in plants and provides perspectives for ongoing cold signaling research.

关键词： EARLY LEAF STAGE FREEZING TOLERANCE CHLOROPLAST DEVELOPMENT negative regulator TRANSCRIPTION FACTORS GENE-EXPRESSION ICE1 STABILITY RICE CALCIUM ARABIDOPSIS

T cell Ig mucin-3 promotes homeostasis of sepsis by negatively regulating the Toll-like receptor response

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T cell Ig mucin-3 promotes homeostasis of sepsis by negative...

第八届全国免疫学学术大会

作者： Xiaomei Yang Xingwei Jiang Guojiang Chen Shaoxia Geng Yan Xiao Chunyan Kang Tingting Zhou Yurong Li Renxi Wang Jiannan Feng Yuanfang Ma Beifen Shen Yan Li Gencheng Han Department of Molecular Immunology Institute of Basic Medical Sciences

Background:Tim-3 is widely involved in regulation of both innate and adaptive immune responses and its dysregulation is associated with autoimmune diseases, tumors, or viral ***, its role in sepsis, a fatal condition characterized by immune disorders, has not been ***:To test whether Tim-3 plays roles in maintaining the homeostasis of ***:Patients with sepsis and mouse model of sepsis were used for this ***:Here, for the first time, we found that 1) blockade and/or downregulation of the Tim-3 pathway correlated with severity of sepsis, and that there is dysregulated downregulation of Tim-3 in the progress of sepsis, suggesting that Tim-3 plays important roles in maintaining the homeostasis of sepsis.2) Blockade and/or downregulation of Tim-3 led to enhanced macrophage activation, which contributed to the uncontrolled inflammatory response in sepsis, while overexpression of Tim-3 in macrophages significantly suppressed Tolllike receptor(TLR)- mediated pro-inflammatory cytokine production, indicating that Tim-3 is a negative regulator of TLR-mediated immune responses.3) Cross-talk between the Tim-3 and TLR4 pathways makes TLR4 an important contributor to Tim-3 mediated negative *** with this, Tim-3 blockade did not significantly affect the progression of sepsis in TLR4 KO mice.4) Tim-3 signaling inhibited LPS-TLR4-mediated NF-κB activation by enhancing PI3K-AKT phosphorylation and by increasing A20 ***:This negative regulator role of Tim-3 reflects a new adaptive compensatory and protective mechanism in sepsis victims, a finding of potential importance for modulating innate responses in these patients.

关键词： Tim-3 Sepsis Toll-like receptors Macrophage negative regulator

Enhanced susceptibility of sorcin deficient mice to sepsis

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Enhanced susceptibility of sorcin deficient mice to sepsis

第十三届全国免疫学学术大会

作者： Yanan Liu Dianzheng Zhao Yongqian Wang Xiaoqi Li Hong Cao Shijun Zheng China Agricultural University College of Veterinary Medicine

Host immune response is tightly controlled by negative regulators to avoid excessive immune reactions for *** previous report showed that FMDV VP1 interacted with cellular protein soluble resistance-related calucium-binding protein(Sorcin) and Sorcin recruited downstream molecular signal transducer and activator of transcription 3(STAT3) in the negative regulation of TNF-α and SeV induced type Ⅰ interferon and NF-κB *** observation suggests that Sorcin act as an important component of host negative regulating ***, the exact role of Sorcin in vivo is still *** we show that Sorcinmice were more susceptible to ***-induced sepsis than Sorcin *** survival rates of Sorcinmice and Sorcin controls were 33.3%(n=15) and 80%(n=15) respectively 96 h post infection with ***(O119)at 5×10 CFU via ***(pnegative regulator to avoid uncontrolled immune response to ***/LPS-induced sepsis.

关键词： sorcin negative regulator immune response inflammation