ROP11 GTPase is a Negative Regulator of Multiple ABA Responses in Arabidopsis

作     者：Zixing Li Jun Kang Ning Sui Dong Liu 

作者机构：MOE Key Laboratory of Bioinformatics School of Life Sciences Tsinghua University Beijing 100084 China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2012年第54卷第3期

页      面：169-179页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 0901[农学-作物学] 

基　　金：supported by the National Basic Research Program of China(973Program)(2009CB119100) the National Natural Science Foundation of China(90717121) 

主　　题：ROP11 GTPase ABA responses negative regulator rop10rop1 double mutant Arabidopsis. 

摘      要：The phytohormone abscisic acid （ABA） plays crucial roles in plant development and plant responses to environmental stresses. Although ABA receptors and a minimal set of core molecular components have recently been discovered, understanding of the ABA signaling pathway is still far from complete. In this work, we characterized the function of ROP11, a member of the plant-specific ROP small GTPases family, in the ABA signaling process. ROP11 is preferentially expressed in guard cells in all plant organs with stomata. Expression of a constitutively active ROP11 （CA-ROP11） suppresses ABA-mediated responses, whereas reduced expression of ROP11 or expression of its dominant-negative form （DN-ROP11） causes the opposite phenotypes. The affected ABA-mediated responses by ROP11 include seed germination, seedling growth, stomatal closure, induction of ABA-responsive genes, as well as plant response to drought stress. Furthermore, we showed that ROP11 and its closest-related family member, ROP10, act in parallel in mediating these responses. ABA treatment does not affect ROP11 transcription and protein abundance; however, it causes the accumulation of CA-ROP11 in the nucleus. These results demonstrated that ROP11 is a negative regulator of multiple ABA responses in Arabidopsis.