Cercospora leaf spot is fast turning into a critically important disease in *** disease is caused by Cercospora coffeicola which significantly reduces productivity and quality of ***,optimum sporulation of Cercospora ...
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Cercospora leaf spot is fast turning into a critically important disease in *** disease is caused by Cercospora coffeicola which significantly reduces productivity and quality of ***,optimum sporulation of Cercospora coffeicola in culture remains a limiting factor for microbial analysis and quantitative studies of Cercospora leaf *** with this challenge,an in-vitro study was conducted at Coffee Research Institute,Manicaland,Zimbabwe to examine growth of Cercospora coffeicola in different nutrient media and to determine the best media for Cercospora coffeicola *** nutrient media were assessed(corn meal agar,oat meal agar,Czapek Dox agar,malt extract agar,yeast extract agar and potato dextrose agar)for the growth of Cercospora *** laboratory-based experiment was duplicated,laid out in a Completely Randomized Design,replicated three times and based on Cercospora coffeicola nutrient *** were collected on radial growth,colour and texture of mycelium at 3 and 6 days after *** were significant differences(pmycelia were found in corn meal *** considering all nutrient media,malt extract agar was found to be the best media for the growth of Cercospora coffeicola *** the basis of our findings,the authors recommend the use of malt extract agar as the primary media for identification and characterisation of Cercospora coffeicola.
Members of the genus Micromonospora show a complex life cycle which normally involves the presence of substrate or vegetative mycelia and sporulation with single spores born on the vegetative hyphae followed by the sy...
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Members of the genus Micromonospora show a complex life cycle which normally involves the presence of substrate or vegetative mycelia and sporulation with single spores born on the vegetative hyphae followed by the synthesis of a dark extracellular polysaccharide. Bergey’s Manual states that micromonosporae rarely produces aerial mycelia (AM) and if so, is considered “sterile”. During the characterisation of novel micromonosporae from the Sea of Cortes, it was observed that AM is produced reproducibly in the presence of certain carbon and/or nitrogen sources. Micromanipulation of the AM subcultured onto fresh media produced colonies;hence, this structure should not be called “sterile”. TEM of the AM producing isolates suggests that the spores also show activity as reported for bacilli of marine origin. This would be the first report of the presence of “inducible” AM in micromonosporae of marine sources and that the spores of this genus have a role other than just dispersal.
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