检索结果-南通市图书馆

Virologica Sinica 2019年第4期34卷 444-453页

作者： Zhanqi Dong Qi Qin Zhigang Hu Peng Chen Liang Huang Xinling Zhang Ting Tian Cheng Lu Minhui Pan State Key Laboratory of Silkworm Genome Biology Southwest UniversityChongqing 400716China Key Laboratory of Sericultural Biology and Genetic Breeding Ministry of AgricultureSouthwest UniversityChongqing 400716China

Recently the developed single guide(sg)RNA-guided clustered regularly interspaced short palindromic repeats/associated protein 9 nuclease(CRISPR/Cas9) technology has opened a new avenue for antiviral therapy. The CRISPR/Cas9 system uniquely allows targeting of multiple genome sites simultaneously. However, there are relatively few applications of CRISPR/Cas9 multigene editing to target insect viruses. To address the need for sustained delivery of a multiplex CRISPR/Cas9-based genome-editing vehicle against insect viruses, we developed a one-vector(pSL1180-Cas9-U6-sgRNA) system that expresses multiple sgRNA and Cas9 protein to excise Bombyx mori nucleopolyhedrovirus(BmNPV) in insect *** screened the immediate-early-1 gene(ie-1), the major envelope glycoprotein gene(gp64), and the late expression factor gene(lef-11), and identified multiple sgRNA editing sites through flow cytometry and viral DNA replication analysis. In addition, we constructed a multiplex editing vector(PSL1180-Cas9-sgIE1-sgLEF11-sgGP64, sgMultiple) to efficiently regulate multiplex gene-editing and inhibit BmNPV replication after viral infection. This is the first report of the application of a multiplex CRISPR/Cas9 system to inhibit insect virus replication. This multiplex system can significantly enhance the potential of CRISPR/Cas9-based multiplex genome engineering in insect virus.

关键词： Bombyx mori Bombyx mori nucleopolyhedrovirus(BmNPV) Antiviral therapeutic CRISPR/Cas9 multigene editing

Transcriptome-wide evolutionary analysis on essential brown algae(Phaeophyceae)in China

在线全文

学校读者我要写书评

暂无评论

Acta Oceanologica Sinica 2014年第2期33卷 13-19页

作者： SUN Jing WANG Liang WU Shuangxiu WANG Xumin XIAO Jingfa CHI Shan LIU Cui REN Lufeng ZHAO Yuhui LIU Tao YU Jun CAS Key Laboratory of Genome Sciences and Information Beijing Key Laboratory of Genome and Precision Medicine TechnologiesBeijing Institute of GenomicsChinese Academy of Sciences Beijing Key Laboratory of Functional Genomics for Dao-di Herbs Beijing Institute of GenomicsChinese Academy of Sciences University of Chinese Academy of Sciences College of Marine Life Science Ocean University of China

Brown algae （Chromista, Ochrophyta, Phaeophyceae） are a large group of multicellular algae that play im-portant roles in the ocean＇s ecosystem and biodiversity. However, poor molecular bases for studying their phylogenetic evolutions and novel metabolic characteristics have hampered progress in the field. In this study, we sequenced the de novo transcriptome of 18 major species of brown algae in China, covering six orders and seven families, using the high-throughput sequencing platform Illumina HiSeq 2000. From the transcriptome data of these 18 species and publicly available genome data of Ectocarpus siliculosus and Phaeodactylum tricornutum, we identified 108 nuclear-generated orthologous genes and clarified the phy-logenetic relationships among these brown algae based on a multigene method. These brown algae could be separated into two clades：Clade Ishigeales-Dictyotales and Clade Ectocarpales-Laminariales-Desmares-tiale-Fucales. The former was at the base of the phylogenetic tree, indicating its early divergence, while the latter was divided into two branches, with Order Fucales diverging from Orders Ectocarpales, Laminariales, and Desmarestiale. In our analysis of taxonomy-contentious species, Sargassum fusiforme and Saccharina sculpera were found to be closely related to genera Sargassum and Saccharina, respectively, while Petalonia fascia showed possible relation to genus Scytosiphon. The study provided molecular evidence for the phylo-genetic taxonomy of brown algae.

关键词： Phaeophyceae transcriptome sequencing multigene phylogeny

From Golden Rice to aSTARice:Bioengineering Astaxanthin Biosynthesis in Rice Endosperm

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2018年第12期11卷 1440-1448页

作者： Qinlong Zhu Dongchang Zeng Suize Yu Chaojun Cui Jiamin Li Heying Li Junyu Chen Runzhao Zhang Xiucai Zhao Letian Chen Yao-Guang Liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Life Sciences South China Agricultural University Guangzhou 510642 China These authors contributed equally to this article

Carotenoids are important phytonutrients with antioxidant properties,and are widely used in foods and feedstuffs as ***,a red-colored ketocarotenoid,has strong antioxidant activity and thus can benefit human ***,astaxanthin is not produced in most higher *** we report the bioengineering of astaxanthin biosynthesis in rice endosperm by introducing four synthetic genes,sZmPSY1,sPaCrtl,sCrBKT,and sHpBHY,which encode the enzymes phytoene synthase,phytoene desaturase,β-carotene ketolase,and β-carotene hydroxylase,*** overexpression of two (sZmPSY1 and sPaCrtl),three (sZmPSY1,sPaCrtl and sCrBKT),and all these four genes driven by rice endosperm-specific promoters established the Carotenoid/ketocarotenoid/astaxanthin biosynthetic pathways in the endosperm and thus resulted in various types of germplasm,from the yellow-grained β-caro- tene-enriched Golden Rice to orange-red-grained Canthaxanthin Rice and Astaxanthin Rice,respectively. Grains Of Astaxanthin Rice were enriched with astaxanthin in the endosperm and had higher antioxidant *** results proved that introduction of a minimal set of four transgenes enables de novo biosynthesis of astaxanthin in therice *** work provides a Successful example for synthetic biology in plants and biofortification in crops;the biofortified rice products generated by this study could be consumed as health-promoting foods and processed tO produce dietary supplements.

关键词： biofortification multigene metabolic engineering astaxanthin biosynthesis rice endosperm

Does the Gonostomum-patterned oral apparatus in hypotrichia carry a phylogenetic signal?Evidence from morphological and molecular data based on extended taxon sampling using three nuclear genes(Ciliophora,Spirotrichea)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2021年第2期64卷 311-322页

作者： Jingyi Wang Yan Zhao Xiaoteng Lu Zhao Lyu Alan Warren Chen Shao Laboratory of Protozoological Biodiversity and Evolution in Wetland College of Life SciencesShaanxi Normal UniversityXi'an 710119China College of Life Sciences Capital Normal UniversityBeijing 100048China Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdao 266003China College of Life Sciences Northwest UniversityXi’an 710069China Department of Life Sciences Natural History MuseumLondon SW75BDUK

The classification of hypotrichs based on the gonostomatid oral structure is widely accepted,but the phylogenetic signal of this character is ***,we infer the species phylogeny of those gonostomatids for which molecular data are available,plus26 new sequences of SSU-rDNA,ITS1-5.8 S-ITS2 and LSU-rDNA *** results indicate that:(i)the endoral is more phylogenetically informative than the paroral;(ii)the structure of the endoral and the Gonostomum-pattern adoral zone of membranelles are plesiomorphies for the hypotrichs sensu stricto;(iii)the group of species possessing these features is monophyletic in all our phylogenetic analyses,except that for the SSU-rDNA;(iv)Schmidingerotrichidae is monophyletic in all trees,suggesting that it is a well-defined family;(v)the Gonostomatidae is polyphyletic in the SSU-rDNA and ITS1-5.8 S-ITS2 trees,with Gonostomum,Cladotricha,Cotterillia,Metagonostomum,Paragonostomum and Wallackia distributed among separate clades,but monophyletic in the LSU-rDNA and concatenated trees;(vi)higher hypotrich taxa such as core urostyloids and core sporadotrichids/stichotrichids might have evolved from species that possessed a gonostomatid oral apparatus.

关键词： Gonostomatidae gonostomatid oral apparatus multigene phylogeny Schmidingerotrichidae

Clinicopathologic analysis of 722 breast cancer patients who met the inclusion criteria of the TAILORx trial

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2019年第24期132卷 2914-2919页

作者： Hong-Yu Xiang Yin-Hua Liu Hong Zhang Shuang Zhang Ling Xin Ling Xu Jing-Ming Ye Ting Li Xue-Ning Duan Qian Liu Breast Disease Center Peking University First HospitalBeijing 100034China Department of Pathology Peking University First HospitalBeijing 100034China

Background:The results of the Trial Assigning IndividuaLized Options for Treatment(TAILORx)suggested that approximately 70%of T1-2N0M0,hormone receptor-positive,human epidermal growth factor receptor 2(HER2)-negative breast cancer patients can avoid chemotherapy and receive only adjuvant endocrine *** conducted a retrospective analysis of the clinicopathologic features and prognostic factors of patients with breast cancer who met the inclusion criteria of the TAILORx ***:According to the enrollment criteria of the TAILORx trial,a retrospective analysis was performed on patients with breast cancer who were treated from January 2008 to December 2015 at Peking University First *** clinicopathologic characteristics of all patients were analyzed,and prognoses were calculated using the Kaplan-Meier method and a Cox proportionate hazards ***:A total of 2430 patients with early stage breast cancer who were admitted at our hospital had complete clinicopathologic data and follow-up *** these patients,722 met the inclusion criteria and were enrolled in the present study,accounting for 29.7%of all *** them,417(57.8%)patients received only adjuvant endocrine therapy(the non-chemo group),and 305(42.2%)patients received adjuvant chemotherapy followed by adjuvant endocrine therapy(the chemo group).No statistically significant difference was observed in overall survival(OS)between the two groups(non-chemo ***:5-year OS:97.9%vs.97.9%,χ^2=1.00,P=0.995;hazard ratio[HR]=1.00,95%confidence interval[CI]:0.46–2.21).A significant difference was observed in disease-free survival(DFS)between the two groups(non-chemo ***:5-year DFS:97.9%vs.94.7%,χ^2=8.65,P=0.003;HR=3.05,95%CI:1.40–6.67).The choice of adjuvant therapy was associated with clinicopathologic factors,such as the age at diagnosis,T stage,histologic grade,the Ki67 index,the presence of intravascular tumor thrombus(P<0.001),pathologic type,and menstrual status(P=0.014

关键词： Adjuvant therapy Early stage breast cancer multigene detection Prognosis TAILORx trial

Phylogeny,taxonomy and diversification events in the Caliciaceae

在线全文

学校读者我要写书评

暂无评论

Fungal Diversity 2017年第1期82卷 221-238页

作者： Maria Prieto Mats Wedin Department of Botany Swedish Museum of Natural HistoryPO Box 5000710405 StockholmSESweden Departamento de Biología y Geología Física y Química InorgánicaUniversidad Rey Juan CarlosC/Tulipan s/n28933 MostolesMadridSpain

Although the high degree of non-monophyly and parallel evolution has long been acknowledged within the mazaediate Caliciaceae(Lecanoromycetes,Ascomycota),a natural re-classification of the group has not yet been *** we constructed a multigene phylogeny of the Caliciaceae-Physciaceae clade in order to resolve the detailed relationships within the group,to propose a revised classification,and to perform a dating *** few characters present in the available fossil and the complex character evolution of the group affects the interpretation of morphological traits and thus influences the assignment of the fossil to specific nodes in the phylogeny,when divergence time analyses are carried *** fossil assignments resulted in very different time estimates and the comparison with the analysis based on a secondary calibration demonstrates that the most likely placement of the fossil is close to a terminal node rather than a basal placement in the Calicium *** dating analysis show two successive events giving rise to main clades of mazaediate taxa within the Caliciaceae,in the Upper-Lower Cretaceous boundary and in the *** a result of this study,Cyphelium is synonymized with Calicium,Acolium is resurrected,and the new genera Allocalicium and Pseudothelomma are *** new combinations are proposed:Acolium karelicum,Acolium marcianum,Allocalicium adaequatum,Calicium carolinianum,Calicium lecideinum,Calicium lucidum,Calicium notarisii,Calicium pinicola,Calicium trachyliodes,Pseudothelomma occidentale,Pseudothelomma ocellatum and Thelomma brunneum.A key for the mazaedium-producing Caliciaceae is included.

关键词： Allocalicium gen nov Calicium fossil Divergence time estimates Lichens multigene Pseudothelomma gen nov

日本东海静冈地区无形体病原菌的p44主要外膜蛋白基因表达区域构造解析

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

内蒙古师范大学学报（自然科学汉文版） 2012年第5期41卷 518-523,530页

作者：乌日图中央民族大学中国少数民族传统医学研究院国家民委-教育部重点实验室北京100081

对日本东海地区无形体病原菌(***)的媒介蜱虫(金沟硬蜱和卵形硬蜱)的唾液腺DNA进行提取,应用分子生物学技术在亚洲地区成功地解读了p44主要外膜蛋白2.87kb的基因序列,并与欧美地区***病原体的p44主要外膜蛋白基因进行序列比对,确定了p4... 详细信息

对日本东海地区无形体病原菌(***)的媒介蜱虫(金沟硬蜱和卵形硬蜱)的唾液腺DNA进行提取,应用分子生物学技术在亚洲地区成功地解读了p44主要外膜蛋白2.87kb的基因序列,并与欧美地区***病原体的p44主要外膜蛋白基因进行序列比对,确定了p44主要外膜蛋白表达基因在欧美以及亚洲地域间的差异性.

关键词： Anaplasma phagocytophilum Humanan aplasmosis p44主要外膜蛋白 p44 multigene family 无形体病原菌媒介蜱虫