Transcriptome-wide evolutionary analysis on essential brown algae(Phaeophyceae)in China

作     者：SUN Jing WANG Liang WU Shuangxiu WANG Xumin XIAO Jingfa CHI Shan LIU Cui REN Lufeng ZHAO Yuhui LIU Tao YU Jun 

作者机构：CAS Key Laboratory of Genome Sciences and InformationBeijing Key Laboratory of Genome and Precision Medicine TechnologiesBeijing Institute of GenomicsChinese Academy of Sciences Beijing Key Laboratory of Functional Genomics for Dao-di HerbsBeijing Institute of GenomicsChinese Academy of Sciences University of Chinese Academy of Sciences College of Marine Life ScienceOcean University of China 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2014年第33卷第2期

页      面：13-19页

核心收录：

学科分类：0710[理学-生物学] 0908[农学-水产] 07[理学] 0707[理学-海洋科学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：The National Natural Science Foundation of China under contract Nos 31140070,31271397 and 41206116 the algal transcrip-tome sequencing was supported by 1KP Project(www.onekp.com) 

主　　题：Phaeophyceae transcriptome sequencing multigene phylogeny 

摘      要：Brown algae （Chromista, Ochrophyta, Phaeophyceae） are a large group of multicellular algae that play im-portant roles in the ocean＇s ecosystem and biodiversity. However, poor molecular bases for studying their phylogenetic evolutions and novel metabolic characteristics have hampered progress in the field. In this study, we sequenced the de novo transcriptome of 18 major species of brown algae in China, covering six orders and seven families, using the high-throughput sequencing platform Illumina HiSeq 2000. From the transcriptome data of these 18 species and publicly available genome data of Ectocarpus siliculosus and Phaeodactylum tricornutum, we identified 108 nuclear-generated orthologous genes and clarified the phy-logenetic relationships among these brown algae based on a multigene method. These brown algae could be separated into two clades：Clade Ishigeales-Dictyotales and Clade Ectocarpales-Laminariales-Desmares-tiale-Fucales. The former was at the base of the phylogenetic tree, indicating its early divergence, while the latter was divided into two branches, with Order Fucales diverging from Orders Ectocarpales, Laminariales, and Desmarestiale. In our analysis of taxonomy-contentious species, Sargassum fusiforme and Saccharina sculpera were found to be closely related to genera Sargassum and Saccharina, respectively, while Petalonia fascia showed possible relation to genus Scytosiphon. The study provided molecular evidence for the phylo-genetic taxonomy of brown algae.