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Accurate nanoelectrode recording of human pluripotent stem cell-derived cardiomyocytes for assaying drugs and modeling disease

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Microsystems & Nanoengineering 2017年第1期3卷 382-388页

作者： Ziliang Carter Lin Allister F.McGuire Paul W.Burridge Elena Matsa Hsin-Ya Lou Joseph C.Wu Bianxiao Cui Department of Applied Physics Stanford UniversityStanfordCA 94305USA Department of Chemistry Stanford UniversityStanfordCA 94305USA Stanford Cardiovascular Institute Stanford UniversityStanfordCA 94305USA Department of Pharmacology and Center for Pharmacogenomics Northwestern UniversityChicagoIL 60611USA

The measurement of the electrophysiology of human pluripotent stem cell-derived cardiomyocytes is critical for their biomedical applications,from disease modeling to drug ***,a method that enables the high-throughput intracellular electrophysiology measurement of single cardiomyocytes in adherent culture is not *** address this area,we have fabricated vertical nanopillar electrodes that can record intracellular action potentials from up to 60 single beating *** access is achieved by highly localized electroporation,which allows for low impedance electrical access to the intracellular ***,we demonstrate that this method provides the accurate measurement of the shape and duration of intracellular action potentials,validated by patch clamp,and can facilitate cellular drug screening and disease modeling using human pluripotent stem *** study validates the use of nanopillar electrodes for myriad further applications of human pluripotent stem cell-derived cardiomyocytes such as cardiomyocyte maturation monitoring and electrophysiology-contractile force correlation.

关键词： cardiomyocytes drug screening electrophysiology human pluripotent stem cells multielectrode array

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Rapid odor perception in rat olfactory bulb by microelectrode array

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第12期13卷 1015-1023页

作者： Jun ZHOU Qi DONG Liu-jing ZHUANG Rong LI Ping WANG Biosensor National Special Lab Key Lab for Biomedical Engineering of Ministry of EducationDepartment of Biomedical EngineeringZhejiang UniversityHangzhou 310027China

Responses of 302 mitral/tufted (M/T) cells in the olfactory bulb were recorded from 42 anesthetized freely breathing rats using a 16-channel microwire electrode *** vapors of four pure chemicals,anisole,carvone,citral and isoamyl acetate were *** aligning spike trains to the initial phase of the inhalation after odor onset,the responses of M/T cells showed transient temporal features including excitatory and inhibitory *** odor-evoked patterns indicated that mammals recognize odors within a short respiration cycle after odor *** to the small amount of information received from a single cell,we pooled results from all responsive M/T cells to study the ensemble *** firing rates of the cell ensembles were computed over 100 ms bins and population vectors were *** high dimension vectors were condensed into three dimensions for visualization using principal component *** trajectories of both excitatory and inhibitory cell ensembles displayed strong dynamics during odor *** distances among cluster centers were enlarged compared to those of the resting ***,we presumed that pictures of odor information sent to higher brain regions were depicted and odor discrimination was completed within the first breathing cycle.

关键词： Rapid odor perception Mitral/tufted cell multielectrode array Odor trajectory

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Precise spatiotemporal patterns of neuronal network activation during persistent reverberatory activity

Precise spatiotemporal patterns of neuronal network activati...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Pak-Ming LAU Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Science University of Science and Technology of China

＜正＞In Hebb’s theory of the cell assembly,short-term memory traces can be stored in closed loop neuronal circuits in form of persistent reverberatory *** previous studies,we have observed persistent network reverberation in cultured hippocampal neurons（Lau and Bi,PNAS,2005）.Here,we investigated the spatiotemporal dynamics of such collective activity of neurons grown on multi-electrode arrays（MEAs）.In this preparation,a reverberation episode consisted of many distinct population bursts defined as reverberation event,each involved the spiking activity of many neurons within a time span of tens of ***,we developed a robust method to define the similarity between two events and use it to find conserved patterns among all *** found that there existed conserved spatiotemporal patterns with high temporal precision in cultured neuronal ***, multiple patterns could co-exist in the same network during spontaneous reverberation and could occur intermittently within the same reverberation *** smaller networks,reverberation could only be evoked by stimulating an input channel,and sometimes only one conserved pattern was *** results suggested that precise spatiotemporal dynamics in neural circuits might allow for more efficient information coding in the brain.

关键词： neural network cell assembly multielectrode array reverberation

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