检索结果-南通市图书馆

Salivary IgA enhancement strategy for development of a nasal-spray anti-caries mucosal vaccine

Science China(Life Sciences) 2013年第5期56卷 406-413页

作者： YAN HuiMin mucosal Immunity Research Group State Key Laboratory of VirologyWuhan Institute of VirologyChinese Academy of Sciences

Dental caries remains one of the most common global chronic diseases caused by Streptococcus mutans,which is prevalent all over the *** caries prevalence of children aged between 5-6 years old in China is still in very high rate.A potent and effective anti-caries vaccine has long been expected for caries prevention but no vaccines have been brought to market till now mainly due to the low ability to induce and maintain protective antibody in oral *** review will give a brief historical retrospect on study of dental caries and pathogenesis,effective targets for anti-caries vaccines,oral immune system and immunization against dental ***,salivary IgA antibodies and the protective responses are discussed in the context of the ontogeny of mucosal immunity to indigenous oral *** methods and advancement for induction of specific anticaries salivary sIgA antibodies and enhancement of specific anti-caries salivary sIgA antibodies by intranasal immunization with a safe effective mucosal adjuvant are *** progress in the enhancement of salivary sIgA antibodies and anticaries protection by intranasal immunization with flagellin-PAc fusion protein will be ***,some of the main strategies that have been used for successful mucosal vaccination of caries vaccine are reviewed,followed by discussion of the mucosal adjuvant choice for achieving protective immunity at oral mucosal membranes for development of a nasal-spray or nasal-drop anti-caries vaccine for human.

关键词： mutans streptococci secretory IgA PAc mucosal immunization caries vaccine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Construction of sperm-specific lactate dehydrogenase DNA vaccine and experimental study of its immuno-contraceptive effect on mice

引用

Science China(Life Sciences) 2008年第4期51卷 308-316页

作者： CHEN Yong ZHANG Duo XIN Na XIONG YongZhong CHEN Ping LI Bo TU XiangDong LAN FengHua Research Laboratory of Medical Genetics PLA Institute of Technology for Birth ControlFetal Health and Child GrowthFuzhou General Hospital of Nanjing Military RegionFuzhou 350025China

Lactate dehydrogenase C4 (LDHC4) is a key enzyme for sperm metabolism. It is distributed specifically in testis and is highly immunogenic. In this study, two DNA vaccines pVAX1-hLDHC and pVAX1-mLDHC were constructed by inserting coding sequences of human and mice LDHC4 into the eukaryotic ex-pression vector pVAX1. The production of LDHC4 specific antibodies was induced in the sera of vac-cinated mice and the reproductive tract secretions of vaccinated female mice through immunization by mucosal surface instillation. Furthermore, the antibody titer increased with the times of immunization. In the mating experiment, the number of newborns of the vaccinated mice reduced significantly and some immunized female mice even lost the ability to bear any offsprings, suggesting that the difference between the immunized and control mice was statistically significant. Sperm agglutination analysis indicated that both the antisera from immunized mice and the reproductive tract secretions of vacci-nated female mice could agglutinate normal sperms. Results of immunohistochemistry showed that the antibodies present in the sera of immunized mice and the reproductive tract secretions of vaccinated female mice could specifically react with LDHC4 antigen, which mainly locates in the cytoplasm, acrosome membrane externa and acrosome capsule of the sperm. Taken together, our results indicated that the constructed contraceptive DNA vaccines did yield immunocontraceptive effects on mice and this would enable clinical trials in near future.

关键词： sperm-specific lactate dehydrogenase DNA vaccine contraceptive vaccine mucosal immunization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Distribution Law of Goblet Cells in the Intestinal Tracts of African Ostrich Chicks

引用

Agricultural Science & Technology 2013年第1期14卷 103-106页

作者：金春艳彭克美刘婷婷王蕾曹维维宋卉刘华珍华中农业大学动物科技-动物医学学院湖北武汉430070

[Objective] This study aimed to investigate the distribution law of different goblet cells in the intestinal tracts of African ostrich chicks. [Method] Alcian blue/pe- riodic acid-schiff reaction （AB/PAS） was adopted to observe and analyze the types and distribution of goblet cells in the intestinal tracts of ostrich chicks. Acid mu- copolysaccharide could be stained blue with alcian blue （AB）, and neutral mu- copolysaccharide could be stained red with periodic acid-schiff reagent （PAS）. [Result] According to AB/PAS results, goblet cells in the intestinal tracts were divided in- to four types： TypeⅠ was pure red, with AB negative result and PAS positive result containing neutral mucoitin; type Ⅱ was pure blue, with AB positive result and PAS negative result containing acidic mucoitin; type Ⅲ was purple reddish, with PAS posi- tive result greater than AB; typeⅣ was purple blue, with AB positive result greater than PAS. Large quantities of goblet cells were found in the intestinal tracts of os- trich chicks, mostly type III and *** quantities of goblet cells were decreased gradually in the duodenum, jejunum and ileum, while the quantities were increased in the cecum, colon and rectum. The goblet cells in the large intestine are more than that in the small intestine. The most quantities of goblet cells were contained in rectum. [Conclusion] These results indicate that the distribution of goblet cells is closely related with the structure and function of intestinal tracts. The mucus secret- ed by the goblet Cells plays a series of important roles in the digestion and mucosal immunization.

关键词： AB/PAS Ostrich chicks Goblet cell mucosal immunization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：