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The dehydrin-like proteins from Sclerotinia sclerotiorum are involved in stress response,virulence and apothecial development via functioning as molecular chaperones

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The dehydrin-like proteins from Sclerotinia sclerotiorum are...

Promising drug targets and associated therapeutic interventions in Parkinson's disease

中国植物病理学会2019年学术年会

作者： Zhu Genglin Liu Jinliang Zhang Xianghui Zhang Yanhua Pan Hongyu College of Plant Sciences Jilin University

Sclerotinia sclerotiorum is the major disease damaging crop,apothecia plays an important role in the life cycle and is the main target of sclerotinia control and prevention.A Forkhead box（FOX）family transcription factor（TF）,SsFoxE2,has been shown to regulate apothecial development in S,sclerotiorum.However,the regulatory mechanisms of how SsFoxE2 affect sexual reproduction remain unclear.In this study,two dehydrin-like proteins,designated as SsDhnl and SsDhn2,were firstly identified as cooperative partners of SsFoxE2.Bioinformatics analysis reveals that SsDhnl and SsDhn2 belong to typical fungal dehydrin-like proteins family containing repeated conserved asparagine-proline-arginine（DPR） motif.Consistent to their predicted biological function of protection against environmental stresses,Ssdhnl and Ssdhn2 knock-out（KO） mutants were impaired in oxidative and osmotic stress resistance,respectively.Furthermore,we demonstrated the expression of Ssdhnl and Ssdhn2 were depended on the mitogen-activated protein kinase（MAPK） SsHogl phosphorylation.Expression analyses indicated that SsDhnl and SsDhn2 are involved in the cellular stress response.Deletion of Ssdhnl or Ssdhn2 resulted in attenuated virulence on common bean leaves.Meanwhile,our results suggested that SsDhnl and SsDhn2 are involved in resistance to plant defense responses via opposing infection stress and protecting effector in the process of infection.Additionally,we found SsFoxE2 could recruit SsDhnl and SsDhn2 into nucleus,and they showed a same expression patterns of which the highest expression level was detected in apothecial stage.Finally,we demonstrated that SsDhnl and SsDhn2 assist SsFoxE2 to form mature sclerotia structure which results in apothecial development.Taken together,our results suggested that SsDhnl and SsDhn2 function as molecular chaperones to affect stress resistance,virulence and sexual development of S.sclerotiorum.

关键词： Sclerotinia sclerotiorum dehydrin-like protein stress response virulence Forkhead box transcription factor sexual development molecular chaperones

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Neural Regeneration Research 2021年第9期16卷 1730-1739页

作者： Sachchida Nand Rai Payal Singh Ritu Varshney Vivek K.Chaturvedi Emanuel Vamanu M.P.Singh Brijesh Kumar Singh Centre of Biotechnology University of AllahabadPrayagrajIndia Department of Zoology Institute of ScienceBanaras Hindu UniversityVaranasiIndia Department of Bioengineering and Chemistry Indian Institute of Technology GandhinagarPalajGujaratIndia Faculty of Biotechnology University of Agronomic Science and Veterinary MedicineBucharestRomania Department of Pathology and Cell Biology Columbia University Medical CenterNew YorkNYUSA

Parkinson's disease(PD) is one of the most debilitating brain diseases. Despite the availability of symptomatic treatments, response towards the health of PD patients remains scarce. To fulfil the medical needs of the PD patients, an efficacious and etiological treatment is required. In this review, we have compiled the information covering limitations of current therapeutic options in PD, novel drug targets for PD, and finally, the role of some critical beneficial natural products to control the progression of PD.

关键词： dopamine epigenetics gene therapies glutamate receptor levodopa molecular chaperones monoamine oxidase B mucuna alpha-synuclein Parkinson's disease striatum substantia nigra

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Down-regulation of p210bcr/ablby curcumin involves disrupting molecular chaperone functions of Hsp90

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Acta Pharmacologica Sinica 2006年第6期27卷 694-699页

作者： Li-xian WU Jian-hua XU~2 Xiu-wang HUANG Kun-zhong ZHANG Cai-xia WEN Yuan-zhong CHEN~3 2 Institute of Clinical Pharmacology,Department of Pharmacology,Fujian Medical University 3 Fujian Institute of Hematology,Fuzhou 350004,China Department of Pharmacology Institute of Clinical Pharmacology Fujian Medical University Fuzhou China Fujian Institute of Hematology Fuzhou China

Aim:To investigate the effects of curcumin（Cur）on p210ber/abllevel in K562 cells,and the relationship between these effects and the molecular chaperone functionsof heat shock protein 90（Hsp90）.Methods:Flow cytometry and Western blotwere used to examine the abundance of p210bcr/abl,Hsp90,p23,Hsp70,and p60Hopin K562 cells treated with Cur.Reverse transcription polymerase chain reaction（RT-PCR）was used to determine the bcr-abl mRNA level in K562 cells treated withCur.After co-immunoprecipitation of p210bcr/abland its molecular chaperones,theimmunoprecipitate was then subjected to Western blot analysis with anti-Hsp90,anti-Hsp70,anti-p23,and anti-p60HoPmAb.Results:An exposure of K562 cells toCur produced time-dependent down-regulation of p210bcr/abl,the inhibition rate ofp210bcr/ablin K562 cells determined by flow cytometry after treatment with Cur 27.2μmol/L for 1h,6h,12h and 24 h was 31.2%,63.7%,81.3% and 94.5%,respectively.In contrast,Cur had almost no influence on bcr-abl mRNA level.Treatment withCur for 24 h reduced the association of p210bcr/ablwith Hsp90/p23 complex,whileincreasing the association of p210bcr/ablwith Hsp70/p60Hopcomplex;however,thetotal protein abundance of Hsp90,p23,and p60Hopin K562 cells had no apparentchange,while Hsp70 increased greatly.Conclusion:Down-regulation ofp210bcr/ablby Cur involves dissociating the binding of p210bcr/ablwith Hspg0/p23complex.In contrast,the association of p210bcr/ablwith HspT0/p60Hopcomplexincreased.

关键词： curcumin molecular chaperones bcr-abl fusion proteins heat shock protein 90

Current understanding of the molecular mechanisms in Parkinson's disease:Targets for potential treatments

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Translational Neurodegeneration 2017年第1期6卷 256-290页

作者： Panchanan Maiti Jayeeta Manna Gary L.Dunbar Field Neurosciences Institute Laboratory for Restorative Neurology Mt.PleasantMI 48859USA Program in Neuroscience Mt.PleasantMI 48859USA Department of Psychology Central Michigan UniversityMt.PleasantMI 48859USA Field Neurosciences Institute St.Mary’s of MichiganSaginawMI 48604USA Department of Biology Saginaw Valley State UniversitySaginawMI 48604USA Department of Physiology University of Tennessee Health Science CenterMemphisTN 38105USA

Gradual degeneration and loss of dopaminergic neurons in the substantia nigra,pars compacta and subsequent reduction of dopamine levels in striatum are associated with motor deficits that characterize Parkinson’s disease(PD).In addition,half of the PD patients also exhibit frontostriatal-mediated executive dysfunction,including deficits in attention,short-term working memory,speed of mental processing,and impulsivity.The most commonly used treatments for PD are only partially or transiently effective and are available or applicable to a minority of patients.Because,these therapies neither restore the lost or degenerated dopaminergic neurons,nor prevent or delay the disease progression,the need for more effective therapeutics is critical.In this review,we provide a comprehensive overview of the current understanding of the molecular signaling pathways involved in PD,particularly within the context of how genetic and environmental factors contribute to the initiation and progression of this disease.The involvement of molecular chaperones,autophagy-lysosomal pathways,and proteasome systems in PD are also highlighted.In addition,emerging therapies,including pharmacological manipulations,surgical procedures,stem cell transplantation,gene therapy,as well as complementary,supportive and rehabilitation therapies to prevent or delay the progression of this complex disease are reviewed.

关键词： Parkinson’s disease Neurodegeneration Protein misfolding molecular chaperones Cell therapy

Oxidative modification of the molecular chaperone family in a PC12 cell model of Parkinson's disease induced by Z-Ile-Glu(OtBu)-Ala-Leucinal

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Neural Regeneration Research 2011年第2期6卷 85-90页

作者： Ying Zhang Yimin Yang Jing Bai Ming Chang Linsen Hu Department of Neurology First Hospital of Jilin University Changchun 130021 Jilin Province China Department of ICU First Hospital of Jilin University Changchun 130021 Jilin Province China

Previous studies have demonstrated that ubiquitin-proteasome system function is significantly decreased in the substantia nigra of Parkinson＇s disease patients. In the present study, proteasome inhibitor Z-Ile-Glu（OtBu）-Ala-Leucinal （PSI） was used to inhibit the function of the ubiquitin-proteasome system in PC12 cells to simulate Parkinson＇s disease. Oxidatively modified proteins were identified to determine pathogenesis of Parkinson＇s disease. Results demonstrated that 24 hours of 10 IJmol/L PSI-treatment in PC12 cells simulated pathological characteristics of Parkinson＇s disease： neuronal degeneration and eosinophilic inclusion formation in neurons. In PSI-treated PC12 cells, three oxidative proteins and a molecular chaperone family member were detected： chaperonin containing t-complex polypeptide 1 subunit 3, glucose-regulated protein 58, and heat shock protein 70. This is the first study to demonstrate oxidative modification of a molecule family in a cell model of Parkinson＇s disease induced with PSI.

关键词： molecular chaperones pathogenesis proteomics protein arbonylation Parkinson＇s disease ubiquitin-proteasome system

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Impact of heat shock on heat shock proteins expression, biological and commercial traits of Bombyx mori

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Insect Science 2006年第4期13卷 243-250页

作者： VASUDHA B. CHAVADI APARNA H. SOSALEGOWDA MANJUNATHA H.B OREGOWDA Departments of Sericulture Dharwad 580003 Karnataka India Departments of Biochemisrty Karnatak University Dharwad 580 003 Karnataka India

We report the thermotolerance of new bivoltine silkworm, Bombyx mori strains NB4D2, KSO1, NP2, CSR2 and CSR4 and differential expression of heat shock proteins at different instars. Different instars of silkworm larva were subjected to heat shock at 35℃, 40℃ and 45℃ for 2 hours followed by 2 hours recovery. Heat shock proteins were analyzed by SDS-PAGE. The impact of heat shock on commercial traits of cocoons was analyzed by following different strategies in terms of acquired thermotolerance over control. Comparatively NP2 exhibited better survivability than other strains. Resistance to heat shock was increased as larval development proceeds in the order of first instar 〉 second instar 〉 third instar 〉 fourth instar 〉 fifth instar in all silkworm strains. Expression of heat shock proteins varies in different instars. 90 kDa in the first, second and third instars, 84 kDa in the fourth instar and 84, 62, 60, 47 and 33 kDa heat shock proteins in fifth instar was observed in response to heat shock. Relative influence of heat shock on commercial traits that correspond to different stages was significant in all strains. In NB4D2, cocoon and shell weight significantly increased to 17.52% and 19.44% over control respectively. Heat shock proteins as molecular markers for evaluation and evolution of thermotolerant silkworm strains for tropics was discussed.

关键词： biological and commercial traits Bombyx mori heat shock heat shockproteins molecular chaperones thermotolerance