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Biophysics Reports 2017年第Z2期3卷 64-72页

作者： Xingguo Liu Liang Yang Qi Long David Weaver Gy?rgy Hajnóczky Key Laboratory of Regenerative Biology Guangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineSouth China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences Department of Pathology Mito Care CenterAnatomy and Cell BiologyThomas Jefferson University

mitochondrial dynamics refers to the processes maintaining mitochondrial homeostasis, including mitochondrial fission, fusion, transport, biogenesis, and mitophagy. mitochondrial dynamics is essential for maintaining the metabolic function of mitochondria as well as their regulatory roles in cell *** this review, we summarize the recently developed imaging techniques for studying mitochondrial dynamics including: mitochondrial-targeted fluorescent proteins and dyes, live-cell imaging using photoactivation, photoswitching and cell fusion, mitochondrial transcription and replication imaging by in situ hybridization, and imaging mitochondrial dynamics by super-resolution microscopy. Moreover,we discuss examples of how to choose and combine proper fluorescent dyes and/or proteins.

关键词： Imaging mitochondrial dynamics Photoactivation Photoswitching Super-resolution microscope

mitochondrial dynamics controls antitumor innate immunity

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Mitochondrial dynamics controls antitumor innate immunity

Effect of AGEs on the expression of mitochondrial dynamics related proteins in cultured human aortic endothelial cells

第十二届全国免疫学学术大会

作者： Jin Jin Zhengjun Gao Life Sciences Institute Zhejiang University

Innate immune cells including macrophages and dendritic cells are critically involved in host defense against infections and inflammatory *** shifts regulated by mitochondrial stress are constantly accompanied with To... 详细信息

关键词： mitochondrial dynamics IL-12 antitumor immunity IRF1 Parkin

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Effect of AGEs on the expression of mitochondrial dynamics r...

Characterization,expression dynamics,and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in Phascolosoma esculenta

2015年老年医学学术年会

作者： Shunrong Zhang Yue Gao Geriatric department Hangzhou First People’s Hospital

Objective: To clarify whether advanced glycation end products (AGEs) can influence the expression of mitochondrial dynamics related proteins Mfn2 and Fis1 in cultured human aortic endothelial cells(HAECs) in vitro. Methods: AGE-BSA was used as AGEs, purchased primary human aortic endothelial cell line was multiplied, and transferred to different passages for subsequent grouping. According to different concentration of AGE-BSA and different duration of AGE-BSA intervention, concentration gradient and time gradient of AGE-BSA intervention experiments were performed. For concentration gradient experiment, HAECs were divided into 4 groups, the concentrations of AGE-BSA in each group were 0 mg/L,50 mg/L,100 mg/L, and 200 mg/L respectively;for time gradient experiment, HAECs were divided into 5 groups with the same concentration of 100 mg/L AGE-BSA , but the intervention times were 0 hour, 6 hours ,12 hours ,24 hours, and 48 hours respectively. RT-PCR method was used to detect the m RNA expression level of Mfn2 and Fis1 genes, whereas western blot method to detect the protein expression level of Mfn1 and Fis1 genes. All data were compared and analyzed with SPSS 16.0 statistical software package. Results: AGEs can decrease the m RNA and protein expression level of Mfn2 in HAECs concentration –dependently(Pmitochondrial dynamicsrelated key protein Mfn2 gene, and increase the expression of Fis1 gene in cultured human aortic endothelial cells in vitro, indicating that

关键词： Advanced glycation end products Human aortic endothelial cell Mitochondria mitochondrial dynamics Gene expression

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Journal of Oceanology and Limnology 2024年第1期42卷 187-200页

作者： Xinming GAO Binbin FENG Chen DU Congcong HOU Shan JIN Daojun TANG Junquan ZHU Key Laboratory of Applied Marine Biotechnology by the Ministry of Education and Key Laboratory of Marine Biotechnology of Zhejiang Province School of Marine SciencesNingbo UniversityNingbo 315211China College of Ecology Lishui UniversityLishui 323000China

Mitochondria undergo morphological changes during spermatogenesis in some *** mechanism and role of mitochondrial morphology regulation,however,remain somewhat *** this study,we analyzed the molecular characteristics,expression dynamics and subcellular localization of optic atrophy protein 1(OPA1),a mitochondrial fusion and cristae maintenance-related protein,to reveal the possible regulatory mechanisms underlying mitochondrial morphology in Phascolosoma esculenta *** full-length cDNA of the *** opa1 gene(Pe-opa1)is 3743 bp in length and encodes 975 amino *** Pe-OPA1 protein is highly conservative and includes a transmembrane domain,a GTPase domain,two helical bundle domains,and a lipid-interacting *** and protein expression was higher in the coelomic fluid(a site of spermatid development)of male *** and increased first and then decreased from March to ***,their expression during the breeding stage was significantly higher than during the non-breeding stage,suggesting that Pe-OPA1 is involved in *** *** Pe-OPA1 protein was more abundant in components consisting of many spermatids than in components without,indicating that Pe-OPA1 mainly plays a role in the spermatid in coelomic ***,Pe-OPA1 was mainly detected in the spermatid *** experiments showed that the Pe-OPA1 are constitutively expressed and co-localized with mitochondria during spermiogenesis,suggesting its involvement in *** *** results provide evidence for Pe-OPA1's involvement in the regulation of mitochondrial morphology during spermiogenesis.

关键词： optic atrophy protein 1(OPA1) spermiogenesis Phascolosoma esculenta mitochondrial dynamics spermatid

Latest assessment methods for mitochondrial homeostasis in cognitive diseases

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Neural Regeneration Research 2024年第4期19卷 754-768页

作者： Wei You Yue Li Kaixi Liu Xinning Mi Yitong Li Xiangyang Guo Zhengqian Li Department of Anesthesiology Peking University Third Hospital Peking University Third Clinical Medical College Beijing Center of Quality Control and Improvement on Clinical Anesthesia Anesthesia and Perioperative Medicine Branch of China International Exchange and Promotive Association for Medical and Health Care(CPAM)

Mitochondria play an essential role in neural function,such as supporting normal energy metabolism,regulating reactive oxygen species,buffering physiological calcium loads,and maintaining the balance of morphology,subcellular distribution,and overall health through mitochondrial *** the recent technological advances in the assessment of mitochondrial structure and functions,mitochondrial dysfunction has been regarded as the early and key pathophysiological mechanism of cognitive disorders such as Alzheimer’s disease,Parkinson’s disease,Huntington’s disease,mild cognitive impairment,and postoperative cognitive *** review will focus on the recent advances in mitochondrial medicine and research methodology in the field of cognitive sciences,from the perspectives of energy metabolism,oxidative stress,calcium homeostasis,and mitochondrial dynamics(including fission-fusion,transport,and mitophagy).

关键词： cognitive disorders mitochondrial dysfunction mitochondrial energy metabolism mitochondrial dynamics mitochondrial transport mitophagy mitochondrial biogenesis oxidative stress calcium homeostasis

mitochondrial dysfunction and quality control lie at the heart of subarachnoid hemorrhage

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Neural Regeneration Research 2024年第4期19卷 825-832页

作者： Jiatong Zhang Qi Zhu Jie Wang Zheng Peng Zong Zhuang Chunhua Hang Wei Li Department of Neurosurgery Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing University Department of Neurosurgery Nanjing Drum Tower HospitalClinical College of Nanjing Medical University

The dramatic increase in intracranial pressure after subarachnoid hemorrhage leads to a decrease in cerebral perfusion pressure and a reduction in cerebral blood *** are directly affected by direct factors such as ischemia,hypoxia,excitotoxicity,and toxicity of free hemoglobin and its degradation products,which trigger mitochondrial *** mitochondria release large amounts of reactive oxygen species,inflammatory mediators,and apoptotic proteins that activate apoptotic pathways,further damaging *** response to this array of damage,cells have adopted multiple mitochondrial quality control mechanisms through evolution,including mitochondrial protein quality control,mitochondrial dynamics,mitophagy,mitochondrial biogenesis,and intercellular mitochondrial transfer,to maintain mitochondrial homeostasis under pathological *** interventions targeting mitochondrial quality control mechanisms have emerged as promising therapeutic strategies for subarachnoid *** review provides an overview of recent research advances in mitochondrial pathophysiological processes after subarachnoid hemorrhage,particularly mitochondrial quality control *** also presents potential therapeutic strategies to target mitochondrial quality control in subarachnoid hemorrhage.

关键词： mitochondrial biogenesis mitochondrial dynamics mitochondrial dysfunction mitochondrial fission and fusion mitochondrial quality control mitophagy subarachnoid hemorrhage

Photobiomodulation provides neuroprotection through regulating mitochondrial fission imbalance in the subacute phase of spinal cord injury

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Neural Regeneration Research 2023年第9期18卷 2005-2010页

作者： Xin Li Xuan-Kang Wang Zhi-Jie Zhu Zhuo-Wen Liang Peng-Hui Li Yang-Guang Ma Tan Ding Kun Li Xiao-Shuang Zuo Cheng Ju Zhi-Hao Zhang Zhi-Wen Song Hui-Lin Quan Jia-Wei Zhang Liang Luo Zhe Wang Xue-Yu Hu Department of Orthopedics Xijing HospitalFourth Military Medical UniversityXi’anShaanxi ProvinceChina 2967 Hospital of People’s Liberation Army Joint Logistic Support Force DalianLiaoning ProvinceChina

Increasing evidence indicates that mitochonarial lission imbalance plays an important role in derayed neuronal cell death. Our previous study round that photo biomodulation improved the motor function of rats with spinal cord ***,the precise mechanism remains *** investigate the effect of photo biomodulation on mitochondrial fission imbalance after spinal cord injury,in this study,we treated rat models of spinal co rd injury with 60-minute photo biomodulation(810 nm,150 mW) every day for 14 consecutive *** electron microscopy results confirmed the swollen and fragmented alte rations of mitochondrial morphology in neurons in acute(1 day) and subacute(7 and 14 days) *** biomodulation alleviated mitochondrial fission imbalance in spinal cord tissue in the subacute phase,reduced neuronal cell death,and improved rat posterior limb motor function in a time-dependent *** findings suggest that photobiomodulation targets neuronal mitochondria,alleviates mitochondrial fission imbalance-induced neuronal apoptosis,and thereby promotes the motor function recovery of rats with spinal cord injury.

关键词： low-level laser therapy mitochondria mitochondrial dynamics mitochondrial fission imbalance neuron photobiomodulation secondary injury spinal cord injury

Aldehyde dehydrogenase 2 preserves mitochondrial morphology and attenuates hypoxia/reoxygenationinduced cardiomyocyte injury

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World Journal of Emergency Medicine 2020年第4期11卷 246-254页

作者： Rui Zhang Meng-yang Xue Bao-shan Liu Wen-jun Wang Xin-hui Fan Bo-yuan Zheng Qiu-huan Yuan Feng Xu Jia-li Wang Yu-guo Chen Department of Emergency Medicine and Chest Pain Center Qilu HospitalShandong UniversityJinanChina Shandong Provincal Clinical Research Center for Emergency and Critical Care Medicine of Shandong Province Institute of Emergency and Critical Care Medicine of Shandong UniversityQilu HospitalShandong UniversityJinanChina Key Laboratory of Emergency and Critical Care Medicine of Shandong Province Key Laboratory of Cardiopulmonary-Cerebral Resuscitation Research of Shandong ProvinceQilu HospitalShandong UniversityJinanChina The Key Laboratory of Cardiovascular Remodeling and Function Research Chinese Ministry of EducationChinese Ministry of Health and Chinese Academy of Medical Sciencesthe State and Shandong Province Joint Key Laboratory of Translational Cardiovascular MedicineQilu HospitalShandong UniversityJinanChina

BACKGROUND:Disturbance of mitochondrial fi ssion and fusion(termed mitochondrial dynamics)is one of the leading causes of ischemia/reperfusion(I/R)-induced myocardial *** studies showed that mitochondrial aldehyde dehydrogenase 2(ALDH2)conferred cardioprotective effect against myocardial I/R injury and suppressed I/R-induced excessive mitophagy in ***,whether ALDH2 participates in the regulation of mitochondrial dynamics during myocardial I/R injury remains ***:In the present study,we investigated the effect of ALDH2 on mitochondrial dynamics and the underlying mechanisms using the H9c2 cells exposed to hypoxia/reoxygenation(H/R)as an in vitro model of myocardial I/R ***:Cardiomyocyte apoptosis was significantly increased after oxygen-glucose deprivation and reoxygenation(OGD/R),and ALDH2 activation largely decreased the cardiomyocyte ***,we found that both ALDH2 activation and overexpression significantly inhibited the increased mitochondrial fission after OGD/***,we found that ALDH2 dominantly suppressed dynamin-related protein 1(Drp1)phosphorylation(Ser616)and adenosine monophosphate-activated protein kinase(AMPK)phosphorylation(Thr172)but not interfered with the expression levels of mitochondrial shaping ***:We demonstrate the protective effect of ALDH2 against cardiomyocyte H/R injury with a novel mechanism on mitochondrial fission/fusion.

关键词： Myocardial hypoxia/reoxygenation injury Aldehyde dehydrogenase 2 mitochondrial fi ssion/fusion mitochondrial dynamics Dynamin-related protein 1

Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis

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Acta Pharmaceutica Sinica B 2023年第2期13卷 577-597页

作者： Yujun Zhou Jingshu Tang Jiaqi Lan Yong Zhang Hongyue Wang Qiuyu Chen Yuying Kang Yang Sun Xinhong Feng Lei Wu Hongtao Jin Shizhong Chen Ying Peng State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China Department of Natural Medicines School of Pharmaceutical SciencesPeking UniversityBeijing 100191China Department of Neurology Beijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijing 102218China New Drug Safety Evaluation Center Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China NMPA Key Laboratory for Safety Research and Evaluation of Innovative Drug Beijing 100050China

Amyotrophic lateral sclerosis(ALS)is a progressive neurodegenerative disease affecting both upper and lower motor neurons(MNs)with large unmet medical *** pathological mechanisms are considered to contribute to the progression of ALS,including neuronal oxidative stress and mitochondrial ***(HNK)has been reported to exert therapeutic effects in several neurologic disease models including ischemia stroke,Alzheimer’s disease and Parkinson’s *** we found that honokiol also exhibited protective effects in ALS disease models both in vitro and in *** improved the viability of NSC-34 motor neuron-like cells that expressed the mutant G93A SOD1 proteins(SOD1-G93A cells for short).Mechanistical studies revealed that honokiol alleviated cellular oxidative stress by enhancing glutathione(GSH)synthesis and activating the nuclear factor erythroid 2-related factor 2(NRF2)-antioxidant response element(ARE)***,honokiol improved both mitochondrial function and morphology via fine-tuning mitochondrial dynamics in SOD1-G93A ***,honokiol extended the lifespan of the SOD1-G93A transgenic mice and improved the motor *** improvement of antioxidant capacity and mitochondrial function was further confirmed in the spinal cord and gastrocnemius muscle in ***,honokiol showed promising preclinical potential as a multiple target drug for ALS treatment.

关键词： Amyotrophic lateral sclerosis Glutathione Honokiol mitochondrial biogenesis mitochondrial dynamics NRF2 Oxidative stress SOD1-G93A

Electroacupuncture preconditioning protects against focal cerebral ischemia/reperfusion injury via suppression of dynamin-related protein 1

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Neural Regeneration Research 2018年第1期13卷 86-93页

作者： Gao-feng Zhang Pei Yang Zeng Yin Huai-long Chen Fu-guo Ma Bin Wang Li-xin Sun Yan-lin Bi Fei Shi Ming-shan Wang Department of Anesthesiology Affiliated Qingdao Municipal Hospital of Qingdao University Department of Public Health Affiliated Qingdao Municipal Hospital of Qingdao University

Electroacupuncture preconditioning at acupoint Baihui （GV20） can reduce focal cerebral ischemia/reperfusion injury. However, the precise protective mechanism remains unknown. mitochondrial fission mediated by dynamin-related protein 1 （Drp1） can trigger neuronal apoptosis following cerebral ischemia/reperfusion injury. Herein, we examined the hypothesis that electroacupuncture pretreatment can regulate Drp1, and thus inhibit mitochondrial fission to provide cerebral protection. Rat models of focal cerebral ischemia/reperfusion injury were established by middle cerebral artery occlusion at 24 hours after 5 consecutive days of preconditioning with electroacupuncture at GV20 （depth 2 mm, intensity 1 mA, frequency 2/15 Hz, for 30 minutes, once a day）. Neurological function was assessed using the Longa neurological deficit score. Pathological changes in the ischemic penumbra on the injury side were assessed by hematoxylin-eosin staining. Cellular apoptosis in the ischemic penumbra on the injury side was assessed by terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling staining. mitochondrial ultrastructure in the ischemic penumbra on the injury side was assessed by transmission electron microscopy. Drp1 and cytochrome c expression in the ischemic penumbra on the injury side were assessed by western blot assay. Results showed that electroacupuncture preconditioning decreased expression of total and mitochondrial Drp1, decreased expression of total and cytosolic cytochrome c, maintained mitochondrial morphology and reduced the proportion of apoptotic cells in the ischemic penumbra on the injury side, with associated improvements in neurological function. These data suggest that electroacupuncture preconditioning-induced neuronal protection involves inhibition of the expression and translocation of Drp1.

关键词： nerve regeneration electroacupuncture focal cerebral ischemia/reperfusion injury dynamin-related protein 1 death-associated protein kinases mitochondrial dynamics mitochondrial ultrastructure apoptosis cytochrome c neural regeneration