检索结果-南通市图书馆

Protective effect of resveratrol on rat cardiomyocyte H9C2 cells injured by hypoxia/reoxygenation by regulating mitochondrial autophagy via PTEN-induced putative kinase protein 1/Parkinson disease protein 2 signaling pathway

引用

Journal of Traditional Chinese Medicine 2022年第2期42卷 176-186页

作者： ZHAO Lixia SUN Wei BAI Decheng Institute of Integrated Traditional Chinese and Western Medicine School of Basic Medical SciencesLanzhou UniversityLanzhou 730000China School of Nursing Gansu University of Chinese MedicineLanzhou 730000China Key Laboratory of Dunhuang Medicine Ministry of EducationGansu University of Chinese MedicineLanzhou 730000China Cardiac Surgery the First Hospital of Lanzhou UniversityLanzhouGansu 730000China

OBJECTIVE:To investigate the protective effect of resveratrol on cardiomyocytes after hypoxia/reoxygenation intervention based on PTEN-induced putative kinase protein 1/Parkinson disease protein 2(PINK1/PARKIN)signaling ***:3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used to detect the effect of resveratrol on the viability of H9C2 cells;the hypoxia/reoxygenation(H/R)model was established in tri-gas incubator;2’,7’-Dichlorofluorescin diacetate staining was used to measure the content of reactive oxygen species(ROS);the changes of mitochondrial membrane potential was determined by 5,5’,6,6’-Tetrachloro-1,1’,3,3’-tetraethyl-imidacarbocyanine iodide staining;the changes of mitochondrial respiratory chain complex activity was evaluated by enzyme activity kits;flow cytometry was used to detect the ratio of apoptotic cells;transmission electron microscope was used to observe the ultrastructure of H9C2 cells;Western blot was used to detect the protein changes of mitochondrial 20 k Da outer membrane protein(TOM20),translocase of inner mitochondrial membrane 23(TIM23),presenilins associated rhomboid-like protein(PARL),PINK1,PARKIN and mitofusin 1(Mfn1),mitofusin 2(Mfn2),phosphotyrosine independent ligand for the Lck SH2 domain of 62 k Da(P62),microtubule-associated protein 1 light chain 3 beta(LC3B);the m RNA levels of PINK1 and PARKIN was detected by quantitative polymerase chain reaction;immunoprecipitation assay was used to detect the interaction between PARKIN and ***:Resveratrol could inhibit the proliferation of H9C2 cells in a time-and concentration-dependent manner;however,pretreatment with low cytotoxic resveratrol could reduce the H/R-induced increase in cellular ROS levels,alleviate the loss of mitochondrial membrane potential induced by H/R,inhibit H/R-induced apoptosis of H9C2 cells,and protect the mitochondrial structure and respiratory chain of H9C2 cells from H/R *** could further increase the leve

关键词： resveratrol myocytes cardiac hypoxia PTEN phosphohydrolase Parkinson disease associated proteins mitochondrial autophagy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Low Selenium and Low Protein Exacerbate Myocardial Damage in Keshan Disease by Affecting the PINK1/Parkin-mediated mitochondrial autophagy Pathway

引用

Current Medical Science 2024年第1期44卷 93-101页

作者： Li-wei ZHANG Hong-qi FENG Song-bo FU Dian-jun SUN Center for Endemic Disease Control Chinese Center for Disease Control and PreventionHarbin Medical UniversityNHC Key Laboratory of Etiology and Epidemiology(Harbin Medical University)Key Lab of Etiology and EpidemiologyEducation Bureau of Heilongjiang ProvinceHarbin 150086China

Objective Keshan disease(KD)is a myocardial mitochondrial disease closely related to insufficient selenium(Se)and protein *** induced putative kinase 1(PINK1)/Parkin mediated mitochondrial autophagy regulates various physiological and pathological processes in the *** study aimed to elucidate the relationship between PINK1/Parkin-regulated mitochondrial autophagy and KD-related myocardial *** A low Se and low protein animal model was *** hundred Wistar rats were randomly divided into 5 groups(control group,low Se group,low protein group,low Se+low protein group,and corn from KD area group).The JC-1 method was used to detect the mitochondrial membrane potential(MMP).ELISA was used to detect serum creatine kinase MB(CK-MB),cardiac troponin I(cTnI),and mitochondrial-glutamicoxalacetic transaminase(M-GOT)***-PCR and Western blot analysis were used to detect the expression of PINK1,Parkin,sequestome 1(P62),and microtubule-associated proteins1A/1B light chain 3B(MAP1LC3B).Results The MMP was significantly decreased and the activity of CK-MB,cTnI,and M-GOT significantly increased in each experimental group(low Se group,low protein group,low Se+low protein group and corn from KD area group)compared with the control group(Pmitochondrial autophagy pathway.

关键词： Keshan disease low selenium and low protein myocardial mitochondrial injury PTEN induced putative kinase 1(PINK1)/Parkin mitochondrial autophagy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of phosphatase and tensin homolog-induced putative kinase 1/E3 ubiquitin ligase Parkin mediated mitochondrial autophagy on chronic kidney disease myocardial injury and the intervention mechanism of Shenshuai recipe(肾衰方)

引用

Journal of Traditional Chinese Medicine 2024年第5期44卷 934-943页

作者： ZHANG Gedi LIU Gengxin GUO Min LUO Fuli YAN Ziyou GE Wei School of Graduate Jiangxi University of Chinese MedicineNanchang 330000China Department of Nephrology Affiliated Hospital of Jiangxi University of Chinese MedicineNanchang 330000China Department of Anorectal Affiliated Hospital of Jiangxi University of Chinese MedicineNanchang 330000China

OBJECTIVE:To study whether Shenshuai recipe(肾衰方,SSR)can play a protective role on chronic kidney disease myocardial injury model through phosphatase and tensin homolog-induced putative kinase 1(PINK1)/E3 ubiquitin ligase Parkin(Parkin)mitochondrial autophagy ***:Forty-eight nephrectomized rats were randomly divided into six groups:sham-operated group,model group,Benazepril group,low,medium and highdose groups of *** rats were given the corresponding intervention for six weeks,then were *** was examined by enzyme linked immunosorbent assay(ELISA).Cardiac ultrasound was used to detect cardiac function in 5/6 nephrectomized *** tissue was examined by light and electron microscopy;PINK1,Parkin,microtubule-associated protein 1 light chain 3 II(LC3B),sequestosome 1(P62),BECN1(Beclin-1)and dynamin-related protein 1(Drp-1)were measured by real time polymerase chain reaction(RT-PCR),Western blot(WB)and immunohistochemistry(IHC).RESULTS:The expression levels of blood urea nitrogen(BUN)and creatinine(SCr)in the model group were significantly higher than those in the sham-operated group,indicating that modeling was *** can protect myocardium by reducing the relative expression of creatine kinase myocardial isoenzyme and hypersensitivity cardiac troponin I(Pmitochondrial autophagy pathway in myocardial tissues in 5/6 nephrectomy CKD myocardial injury rats was *** can activate PINK1/Parkin mitochondrial autophagy to enhance mitochondrial autophagy,and p

关键词： renal insufficiency chronic PTEN phosphohydrolase ubiquitin-protein ligases myocardial injury mitochondrial autophagy Shenshuai recipe

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Fang’s hypoglycemic formula improves insulin resistance in type 2 diabetes mellitus by regulating mitochondrial autophagy

引用

Integrative Medicine Discovery 2024年第16期8卷 1-7页

作者： Jia Zhu Xu-Dong Chen Jie Zhao Department of Respiratory Jiaxing Hospital of Traditional Chinese MedicineJiaxing 314001China Department of Endocrinology Jiaxing Hospital of Traditional Chinese MedicineJiaxing 314001China Department of Endocrinology The First Affiliated Hospital of Yunnan University of Chinese MedicineKunming 650500China

Background:To investigate the pharmacological effects of Fangshi Jiangtang decoction(FSJT)on type 2 diabetes mellitus(T2DM)model rats and explore its mechanism of action from the perspective of mitochondrial ***:Sixty Sprague Dawley rats were randomly divided into six groups after one week of adaptive feeding:Control group,T2DM model group,metformin group(0.2 g/kg by gavage),and FSJT low,medium,and high dose groups(9.5,19,38 g/kg by gavage).Except for the Control group,the other five groups were given a high-fat *** treatment lasted for 8 weeks,and blood glucose levels were measured *** weeks later,blood samples were collected from the rats,and serum was separated for the determination of HbA1c,oral glucose tolerance test,and homeostatic model assessment for insulin resistance *** pancreas of the rats was collected,weighed,and *** same part of the pancreas was used for *** were used to detect triglycerides,total cholesterol,interleukin-1β,interleukin-6,tumor necrosis factor-α,malondialdehyde,glutathione peroxidase,and superoxide dismutase in pancreatic tissue to assess the effects of FSJT on inflammation and oxidative stress in T2DM *** blot analysis was performed to detect the expression of VDAC1,TOM20,COXⅣ,PINK1,Parkin,beclin1,light chain 3,and selective autophagy adaptor protein P62 to evaluate the effects of FSJT on mitochondrial autophagy in T2DM model ***:Compared with the T2DM model group,FSJT intervention significantly reduced blood glucose,HbA1c,oral glucose tolerance test,and homeostatic model assessment for insulin resistance index in T2DM model rats,alleviated pancreatic tissue lesions,reduced levels of total cholesterol,triglycerides,interleukin-1β,interleukin-6,tumor necrosis factor-α,and malondialdehyde,increased glutathione peroxidase and superoxide dismutase activities,downregulated the expression of VDAC1,TOM20,COXⅣ,and P62 proteins,and upregulated the expression of PINK1,

关键词： Fangshi Jiangtang decoction type 2 diabetes mellitus mitochondrial autophagy oxidative stress

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The role of mitochondrial autophagy in Doxorubicininduced cardiotoxicity

引用

South China Journal of Cardiology 2018年第1期19卷 69-75页

作者：聂佩张金国 Jining Medical University Department of Cardiology the Affiliated Hospital of Jining Medical University

Background Doxorubicin is a widely used drug in all kinds of chemotherapy, but its application is limited by its cardiac toxicity to some extent. Most of scholars have studied doxorubicin induced myocardial injury and cardiomyocytes death, but the specific mechanism remains unclear. autophagy is a metabolic pathway of degrad- ing longevity protein and organelles by lysosome, especially selective autophagy which called mitochondrial au- tophagy exists in various cells. The normal range of mitochondrial autophagy helps to maintain the physiological function, otherwise, could lead to the development of disease. Recent studies have suggested that mitochondrial autophagy is involved in the progress of doxorubicin-induced cardiotoxicity. This article reviews the role of mito- chondrial autophagy in doxorubicin-induced cardiotoxicity, and how mitochondrial autophagy is involved in the occurrence and development of the progress, in order to provide a theoretical basis of prevention and treatment.

关键词： mitochondrial autophagy doxorubicin cardiotoxicity effect

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of Qingguang’an Granules on mitochondrial autophagy of retinal ganglion cells in rats with chronic ocular hypertension

引用

Digital Chinese Medicine 2022年第3期5卷 295-304页

作者： TANG Yu ZHU Bingyao SHI Jian LIU Qianhong CHEN Lihao PENG Qinghua PENG Jun YAO Xiaolei Department of Ophthalmology The First Hospital of Hunan University of Chinese MedicineChangshaHunan 410007China Graduate School Hunan University of Chinese MedicineChangshaHunan 410208China Hunan Provincial Key Laboratory for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine ChangshaHunan 410208China Hunan Provincial Engineering and Technological Research Center for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine and Protecting Visual Function ChangshaHunan 410208China

Objective To investigate the effect and underlying mechanism of Qingguang’an Granules(青光安颗粒剂,QGAG)on mitochondrial autophagy(mitophagy)of retinal ganglion cells(RGCs)in rats with chronic ocular hypertension(COH).Methods Sixty Sprague Dawley(SD)rats,half males and half females,were randomly assigned to three groups:the control,model,and QGAG(2.5 g/kg)groups,with 20 rats in each ***’model of COH was established by cauterizing episcleral veins in the model group and QGAG *** weeks after successful modeling,rats in the QGAG group were intra-gastrically administered with QGAG,while rats in the control group and the model group received an equal dose of normal *** three months of intragastric administration,intraocular pressure(IOP)of all rats was *** mitophagy was monitored by the immunofluorescence method,the mitochondrial membrane potential was measured using the JC-1 method,and the morphological changes of mitophagy in RGCs were observed by transmission electron ***,rat RGCs were labeled using the fluorescent gold method,and RGCs density in each group was ***,RGCs apoptosis was observed by TdT-mediated dUTP Nick-End Labeling(TUNEL)***,the expression levels of Parkin,optineurin,microtubule-associated protein 1 light chain 3-Ⅱ/microtubule-associated protein 1 light chain 3-Ⅰ(LC3-Ⅱ/LC3-Ⅰ),recombinant lysosomal associated membrane protein 1(LAMP1),and B-cell lymphoma-2(Bcl-2)in RGCs were determined by Western blot *** corresponding mRNAs were detected through quantitative real-time polymerase chain reaction(qRT-PCR).Results The QGAG reduced IOP in COH rats,and inhibited mitophagy and apoptosis of RGCs(P<0.05).Besides,the QGAG significantly increased the expression levels of Parkin and Bcl-2(P<0.05),and inhibited the expression levels of optineurin,LAMP1,and LC3-Ⅱ/LC3-Ⅰ(P<0.05)in RGCs of COH *** The QGAG can inhibit mitophagy in RGCs of COH rats and show a protective effect agains

关键词： Glaucoma Qingguang’an Granules(青光安颗粒剂,QGAG) Retinal ganglion cells(RGCs) mitochondrial autophagy Parkin/PINK1 pathway

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Discussion on the Relationship Between Senescence Caused by Pi(Spleen)Deficiency and mitochondrial autophagy

引用

World Journal of Integrated Traditional and Western Medicine 2022年第2期8卷 20-24页

作者： JIN Tian-tian TANG Jie KONG Ming-wang SHI He-yuan Institute of Basic Theory of Traditional Chinese Medicine Hubei University of Traditional Chinese MedicineWuhan 430065China

The Pi(Spleen)is responsible for the formation and transportation,and it is in the *** is the source of qi and blood generation and transformation,so it has an essential *** the qi and blood are biochemically passive,the functions of the human body will age *** are for energy synthesis and have always been a hot topic in modern medical *** of their functions are similar to those of the Pi in Chinese *** Chinese medicine,strengthening the Pi is to enhance the Pi’s ability to transport and transform,and to speed up the Pi,which is similar to the ability of mitochondria to maintain the ability to remove damaged *** can speed up the body’s ability to absorb nutrients and delay aging.

关键词： Huangdi Neijing Delays aging Pi(Spleen)deficiency mitochondrial autophagy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Mitophagy deficiency activates stimulator of interferon genes activation and aggravates pathogenetic cardiac remodeling

引用

Genes & Diseases 2024年第6期11卷 381-394页

作者： Guoxiang Zhou Xiaowen Wang Mingyu Guo Can Qu Lei Gao Jiang Yu Yuanjing Li Suxin Luo Qiong Shi Yongzheng Guo Division of Cardiology The First Affliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Cardiothoracic Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Pharmacy The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China The Department of Laboratory Medicine M.O.E.Key Laboratory of Laboratory Medical DiagnosticsChongqing Medical UniversityChongqing 400016China

Stimulator of interferon genes(STING)has recentlybeen found to play a crucial role in cardiac sterile inflammation and *** role of stimulator of interferon genes(STING)in cardiac sterile inflammation and dysfunction has been recently *** study aims to examine the involvement of STING in pathological cardiac remodeling and the mechanisms that govern the activation of the STING *** investigate this,transverse aortic constriction(TAC)was performed on STING knockout mice to induce pressure over-load-induced cardiac ***,cardiac function,remodeling,and inflammation levels were *** STING pathway was found to be activated in the pressure overload-stressed heart and angiotensin II(Ang Il)-stimulated cardiac *** of STING expression led to a significant reduction in inflammatory responses,mitochondrial fragmenta-tion,and oxidative stress in the heart,resulting in attenuated cardiac remodeling and ***,the exacerbation of pressure overload-induced sTING-mediated inflammation and pathological cardiac remodeling was observed when mitophagy was sup-pressed through the silencing of Parkin,an E3 ubiquitin *** together,these findings indicate that STING represents a newly identified and significant molecule implicated in the process of pathological cardiac remodeling and that mitophagy is an upstream mechanism that regulates STING *** STING may therefore provide a novel therapeutic strategy for pathological cardiac remodeling and heart failure.

关键词： Cardiac remodeling mitochondrial autophagy mtDNA Sterile inflammation STING

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bloodletting Acupuncture at Jing-Well Points on Hand Induced autophagy to Alleviate Brain Injury in Acute Altitude Hypoxic Rats by Activating PINK1/Parkin Pathway

引用

Chinese Journal of Integrative Medicine 2023年第10期29卷 932-940页

作者： LI Yong-ping LI Meng-xin WANG Chao LI Yun-di SA Yu-ping GUO Yi Medical College of Qinghai University Xining810001China Qinghai Provincial Key Laboratory of Traditional Chinese Medicine Research for Glucolipid Metabolic Diseases Xining810001China Research Center of Experimental Acupuncture Science Tianjin University of Traditional Chinese MedicineTianjin301617China

Objective: To explore the protective effect of bloodletting acupuncture at twelve Jing-well points on hand(BAJP) on acute hypobaric hypoxia(AHH)-induced brain injury in rats and its possible ***: Seventy-five Sprague Dawley rats were divided into 5 groups by a random number table(n=15),including control, model, BAJP, BAJP+3-methyladenine(3-MA), and bloodletting acupuncture at non-acupoint(BANA, tail tip blooding) groups. After 7-day pre-treatment, AHH models were established using hypobaric oxygen chambers. The levels of S100B, glial fibrillary acidic protein(GFAP), superoxide dismutase(SOD), and malondialdehyde(MDA) in serum were measured by enzyme-linked immunosorbent assay. Hematoxylin-eosin staining and the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling method were used to assess hippocampal histopathology and apoptosis. Transmission electron microscopy assay was used to observe mitochondrial damage and autophagosomes in hippocampal tissues. Flow cytometry was used to detect mitochondrial membrane potential(MMP). The mitochondrial respiratory chain complexes Ⅰ, Ⅲ and Ⅳ activities and ATPase in hippocampal tissue were evaluated, respectively. Western blot analysis was used to detect the protein expressions of Beclin1, autophagy protein 5(ATG5), microtubule-associated protein 1 light chain 3 beta(LC3B), phosphatase and tensin homolog induced kinase 1(PINK1), and Parkin in hippocampal tissues. The mRNA expressions of Beclin1, ATG5 and LC3-Ⅱ were analyzed by quantitative real-time polymerase chain reaction. Results: BAJP treatment reduced hippocampal tissue injury and inhibited hippocampal cell apoptosis in AHH rats. BAJP reduced oxidative stress by decreasing S100B, GFAP and MDA levels and increasing SOD level in the serum of AHH rats(Pmitochondrial respiratory chain complexes Ⅰ, Ⅲ and Ⅳ activities, and the mitochondrial ATPase activity in AHH rats(all P<0.01). BAJP improve

关键词： acute hypobaric hypoxia bloodletting acupuncture at Jing-well points mitochondrial autophagy PINK1/Parkin signaling mitochondrial damage Chinese medicine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chemical Synthesis of Structurally Defined Phosphorylated Ubiquitins Suggests Impaired Parkin Activation by Phosphorylated Ubiquitins with a Non-Phosphorylated Distal Unit

引用

CCS Chemistry 2019年第5期1卷 476-489页

作者： Man Pan Qingyun Zheng Shuai Gao Qian Qu Yuanyuan Yu Ming Wu Huan Lan Yulei Li Sanling Liu Jiabin Li Demeng Sun Lining Lu Tian Wang Wenhao Zhang Jiawei Wang Yiming Li Hong-Gang Hu Changlin Tian Lei Liu Tsinghua-Peking Center for Life Sciences Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologyCenter for Synthetic and Systems BiologyState Key Laboratory of Chemical OncogenomicsDepartment of ChemistryTsinghua UniversityBeijing 100084China State Key Laboratory of Chemical Oncogenomics Key Laboratory of Chemical BiologyGraduate School at ShenzhenTsinghua UniversityShenzhenGuangdong 518055China Institute of Translational Medicine Shanghai UniversityShanghai 200444China Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of ChinaHefei 230027China School of Biological and Medical Engineering Hefei University of TechnologyHefeiAnhui 230009China

Mutations in genes encoding PINK1(PTEN-induced kinase 1)and Parkin(E3 ubiquitin ligase)are identified in familial Parkinson’s ***,it remains unclear whether the phosphorylated Ub chains activate wild-type Parkin(w-Pa... 详细信息

关键词： chemical protein synthesis ubiquitin phosphorylation Parkin mitochondrial autophagy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：