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DNA Barcoding and Mini-DNA Barcoding Reveal mislabeling of Salmonids in Different Distribution Channels in the Qingdao Area

引用

Journal of Ocean University of China 2021年第6期20卷 1537-1544页

作者： HAN Cui DONG Shuanglin LI Li GAO Qinfeng ZHOU Yangen Key Laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266235China

There is an increasing demand for salmonid authentication due to the globalization of the salmonid *** barcoding and mini-DNA barcoding are widely used for identifying fish species based on a fragment of the mitochondrial cytochrome c oxidase subunit I(COI)*** this study,rainbow trout(Oncorhynchus mykiss),steelhead trout(***),and Atlantic salmon(Salmo salar)collected from two salmonid aquaculture bases in China were authenticated by DNA barcoding(about 650 bp)and mini-DNA barcoding(127 bp)to evaluate the accuracy of the two methods in the identification of different salmonid *** results revealed that both methods could effectively distinguish *** and *** with 100%***,the two methods failed to separate rainbow trout(***)and steelhead trout(***),which are the same species but cultured in different water ***,salmonid samples from three main distribution channels in the Qingdao area(traditional supermarkets,online supermarkets,and sushi bars)were identified by the two *** of *** with *** was discovered,and the 27.78%overall substitution rate of salmonids in the Qingdao area was higher than those in other regions reported in previous *** addition,the mislabeling rates of salmonids from traditional supermarkets,online supermarkets,and sushi bars were compared in this *** mislabeling rate was significantly greater in sushi bars(50%)than in the other two channels(16.67%),suggesting that stronger monitoring and enforcement measures are necessary for the aquatic food catering industry.

关键词： salmonid DNA barcoding mini-DNA barcoding species authentication mislabeling rate

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