DNA Barcoding and Mini-DNA Barcoding Reveal Mislabeling of Salmonids in Different Distribution Channels in the Qingdao Area

作     者：HAN Cui DONG Shuanglin LI Li GAO Qinfeng ZHOU Yangen HAN Cui;DONG Shuanglin;LI Li;GAO Qinfeng;ZHOU Yangen

作者机构：Key Laboratory of MaricultureMinistry of EducationOcean University of ChinaQingdao 266003China Function Laboratory for Marine Fisheries Science and Food Production ProcessesQingdao National Laboratory for Marine Science and TechnologyQingdao 266235China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2021年第20卷第6期

页      面：1537-1544页

核心收录：

学科分类：120301[管理学-农业经济管理] 12[管理学] 1203[管理学-农林经济管理] 0908[农学-水产] 09[农学] 

基　　金：the National Key Research and Development Program of China(No.2019YFD0901000) the Natural Science Foundation of Shandong Pro-vince,China(No.ZR2020MC194) 

主　　题：salmonid DNA barcoding mini-DNA barcoding species authentication mislabeling rate 

摘      要：There is an increasing demand for salmonid authentication due to the globalization of the salmonid *** barcoding and mini-DNA barcoding are widely used for identifying fish species based on a fragment of the mitochondrial cytochrome c oxidase subunit I(COI)*** this study,rainbow trout(Oncorhynchus mykiss),steelhead trout(***),and Atlantic salmon(Salmo salar)collected from two salmonid aquaculture bases in China were authenticated by DNA barcoding(about 650 bp)and mini-DNA barcoding(127 bp)to evaluate the accuracy of the two methods in the identification of different salmonid *** results revealed that both methods could effectively distinguish *** and *** with 100%***,the two methods failed to separate rainbow trout(***)and steelhead trout(***),which are the same species but cultured in different water ***,salmonid samples from three main distribution channels in the Qingdao area(traditional supermarkets,online supermarkets,and sushi bars)were identified by the two *** of *** with *** was discovered,and the 27.78%overall substitution rate of salmonids in the Qingdao area was higher than those in other regions reported in previous *** addition,the mislabeling rates of salmonids from traditional supermarkets,online supermarkets,and sushi bars were compared in this *** mislabeling rate was significantly greater in sushi bars(50%)than in the other two channels(16.67%),suggesting that stronger monitoring and enforcement measures are necessary for the aquatic food catering industry.