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microtubule stability and MAP1B upregulation control neuritogenesis in CAD cells

Acta Pharmacologica Sinica 2006年第9期27卷 1119-1126页

作者： Wen LI~(2,5)Jin-tang XIA~3 Yue FENG~(4,5)2 Laboratory of Surgery,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China 3 Department of General Surgery,The First People's Hospital of Guangzhou,Guangzhou 510180,China 4 Department of Pharmacology,Emory University School of Medicine,USA Laboratory of Surgery The First Affiliated Hospital Sun Yat-sen University Guangzhou China Department of General Surgery The First People's Hospital of Guangzhou Guangzhou China Department of Pharmacology Emory University School of Medicine USA

Aim:To study the role of microtubule dynamics and microtubule associated pro-tein 1B(MAP1B)in regulation of the neurite extension in CAD catecholaminergicneuronal cell ***:The neuritogenesis of the CAD cells was abolishedby inhibiting microtubule polymerization with nocodazole and by blocking micro-tubule depolymerization with ***1B and tubulin protein expression wasdetected by Western *** staining of tubulins was observedby fluorescent and confocal ***:microtubule dynamics wasessential for CAD neurite *** analysis revealed that neurite exten-sion was much more sensitive to nocodazole than to taxol,suggesting a func-tional requirement for highly active microtubule assembly.A remarkableupregulation of MAP IB protein was detected during neurite extension accompa-nied with increased microtubule ***:Upregulation of MAP1Bleads to the stabilization of newly formed microtubules in the developing neurites,which in turn promotes neurite extension.

关键词： microtubule stability microtubule associated protein 1B neuritogenesis

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A cilium-independent role for intraflagellar transport 88 in regulating angiogenesis

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Science Bulletin 2021年第7期66卷 727-739,M0004页

作者： Yang Yang Miao Chen Jingrui Li Renjie Hong Jia Yang Fan Yu Te Li Song Yang Jie Ran Chunyue Guo Yi Zhao Yi Luan Min Liu Dengwen Li Songbo Xie Jun Zhou College of Life Sciences State Key Laboratory of Medicinal Chemical BiologyKey Laboratory of Bioactive Materials of the Ministry of EducationTianjin Key Laboratory of Protein ScienceNankai UniversityTianjin 300071China College of Life Sciences Shandong Provincial Key Laboratory of Animal Resistance BiologyCollaborative Innovation Center of Cell Biology in Universities of ShandongInstitute of Biomedical SciencesShandong Normal UniversityJinan 250014China Department of Translational Medicine Center The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China

Endothelial cilia are microtubule-based hair-like protrusions in the lumen of blood vessels that function as fluid mechanosensors to regulate vascular hemodynamics. However, the functions of endothelial cilia in vascular development remain controversial. In this study, depletion of several key proteins responsible for ciliogenesis allows us to identify a cilium-independent role for intraflagellar transport 88(IFT88) in mammalian angiogenesis. Disruption of primary cilia by heat shock does not affect the angiogenic process. However, depletion of IFT88 significantly inhibits angiogenesis both in vitro and in vivo. IFT88 mediates angiogenesis by regulating the migration, polarization, proliferation, and oriented division of vascular endothelial cells. Further mechanistic studies demonstrate that IFT88 interacts with c-tubulin and microtubule plus-end tracking proteins and promotes microtubule stability. Our findings indicate that IFT88 regulates angiogenesis through its actions in microtubule-based cellular processes, independent of its role in ciliogenesis.

关键词： IFT88 Angiogenesis Cilium Cell migration Cell division microtubule stability

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CD147 supports paclitaxel resistance via interacting with RanBP1

CD147 supports paclitaxel resistance via interacting with Ra...

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2021年中国肿瘤标志物学术大会暨第十五届肿瘤标志物青年科学家论坛

作者： Gang Nan Hongyong Cui 空军军医大学

Objectives: Though the great success of paclitaxel, the variable response activity of patients to the drug limits its clinical utility and the precise mechanisms underlying the variable response to paclitaxel remain largely unknown. This study aims to verify the role and the underlying mechanisms of CD147 in paclitaxel resistance. Immunostaining was used to analyze human ovarian cancer tissues. RNAsequencing was used to identify downstream ***: Annexin V-FITC/propidium iodide and terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL) staining were used to detect apoptosis. Co-immunoprecipitation(Co-IP),fluorescence resonance energy transfer(FRET) and surface plasmon resonance(SPR) were performed to determine protein interactions. Fluorescence recovery after photobleaching(FRAP) was performed to measure the speed of microtubule turnover. Xenograft tumor model was established to evaluate sensitivity of cancer cells to paclitaxel in ***: In vitro and in vivo assays showed that silencing CD147 sensitized the cancer cells to paclitaxel treatment. CD147 protected cancer cells from paclitaxel-induced caspase-3 mediated apoptosis regardless of p53 status. Truncation analysis showed that the intracellular domain of CD147(CD147 ICD) was indispensable for CD147-regulated sensitivity to paclitaxel. Via screening the interacting proteins of CD147 ICD, Ran binding protein 1(RanBP1) was identified to interact with CD147 ICD via its C-terminal tail. Furthermore, we showed that RanBP1 mediated CD147-regulated microtubule stability and dynamics as well as response to paclitaxel ***: These results demonstrated that CD147 regulated paclitaxel response by interacting with the C-terminal tail of RanBP1 and targeting CD147 may be a promising strategy for preventing paclitaxel resistant.

关键词： Ovarian cancer non-small-cell lung cancer paclitaxel CD147 RanBP1 microtubule stability

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