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Regenerative Biomaterials 2022年第1期9卷 560-571页

作者： Yajing Zhang Yan Huang Rong Chen Shulin Chen Xiaoying Lü State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast University2#Si PailouNanjing 210096China

The aim of this article was to study the toxicity mechanism of nickel ions(Ni^(2+))on L929 cells by combining proteomics and ***,iTRAQ-based proteomics and LC/MS metabolomics analyses were used to determine the protein and metabolite expression profiles in L929 cells after treatment with 100μMNi^(2+)for 12,24 and 48 h.A total of 177,2191 and 2109 proteins and 40,60 and 74 metabolites were found to be differentially ***,the metabolic pathways in which both differentially expressed proteins and metabolites were involved were identified,and three pathways with proteins and metabolites showing upstream and downstream relationships were affected at all three time ***,the protein-metabolite-metabolic pathway network was constructed,and two important metabolic pathways involving 4 metabolites and 17 proteins were ***,the functions of the important screened metabolic pathways,metabolites and proteins were investigated and experimentally ***^(2+)mainly affected the expression of upstream proteins in the glutathione metabolic pathway and the arginine and proline metabolic pathway,which further regulated the synthesis of downstream metabolites,reduced the antioxidant capacity of cells,increased the level of superoxide anions and the ratio of GSSG to GSH,led to oxidative stress,affected energy metabolism and induced apoptosis.

关键词： nickel ion(Ni2t) proteomics metabolomics protein-metabolite-metabolic pathway network

Optimization of the quenching method for metabolomics analysis of Lactobacillus bulgaricus

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第4期15卷 333-342页

作者： Ming-ming CHEN Ai-li LI Mao-cheng SUN Zhen FENG Xiang-chen MENG Ying WANG MOE Key Laboratory of Dairy Science College of Food ScienceNortheast Agricultural University School of Public Health Jilin Medical College

This study proposed a quenching protocol for metabolite analysis of Lactobacillus delbrueckii *** cells were quenched with 60% methanol/water,80% methanol/glycerol,or 80% methanol/*** effect of the quenching process was assessed by the optical density（OD）-based method,flow cytometry,and gas chromatography-mass spectrometry（GC-MS）.The principal component analysis（PCA） and orthogonal partial least squares-discriminant analysis（OPLS-DA） were employed for metabolite *** results indicated that quenching with 80% methanol/water solution led to less damage to the *** cells,characterized by the lower relative fraction of prodium iodide（PI）-labeled cells and the higher OD recovery *** GC-MS analysis,higher levels of intracellular metabolites（including focal glutamic acid,aspartic acid,alanine,and AMP） and a lower leakage rate were detected in the sample quenched with 80% methanol/water compared with the *** conclusion,we suggested a higher concentration of cold methanol quenching for *** metabolomics due to its decreasing metabolite leakage.

关键词： metabolomics Quenching method Lactobacillus bulgaricus Leakage

Research progress and perspective in metabolism and metabolomics of psoriasis

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Chinese Medical Journal 2020年第24期133卷 2976-2986页

作者： Ni Lian Li-Qing Shi Zhi-Min Hao Min Chen Department of Dermatology Hospital for Skin Diseases(Institute of Dermatology)Chinese Academy of Medical Sciences&Peking Union Medical CollageNanjingJiangsu 210042China

Psoriasis is considered a systemic disease associated with metabolic abnormalities,and it is important to understand the mechanisms by which metabolism affects pathophysiological processes both holistically and *** are closely related to disease phenotypes,especially in systemic diseases under multifactorial *** emergence of metabolomics has provided information regarding metabolite changes in lesions and circulation and deepened our understanding of the association between metabolic reprogramming and *** has great potential for the development of effective biomarkers for clinical diagnosis,therapeutic monitoring,prediction of the efficacy of psoriasis management,and further discovery of new metabolism-based therapeutic targets.

关键词： Psoriasis Metabolism metabolomics Biomarkers

Integrated metabolomics analysis of the effect of PPARδ agonist GW501516 on catabolism of BCAAs and carboxylic acids in diabetic mice

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Chinese Chemical Letters 2021年第7期32卷 2197-2202页

作者： Li Xiang Juan Nie Li Wang Yu Wang Jingchun Shi Juntong Wei Chi-Wai Lau Zongwei Cai Yu Huang Heart and Vascular Institute School of Biomedical Sciences and Shenzhen Research Institute.Chinese University of Hong KongHong KongChina State Key Laboratory of Environmental and Biological Analysis and Department of Chemistry Hong Kong Baptist UniversityHong KongChina

The peroxisome proliferator-activated receptor(PPARδ)agonists are reported to improve insulin sensitivity,reduce glucose levels,and alleviate dysfunctional lipid metabolism in animal models of type 2 diabetes ***,the underlying mechanisms remain incompletely *** plays an essential role in the biological *** of metabolic changes in response to disease conditions or drug treatment is critical for better understanding of the pathophysiological *** this study,metabolic profiling analysis by gas chromatography-mass spectrometry integrated with targeted analysis by liquid chro matography-mass spectrometry was carried out in plasma samples of db/db diabetic mice after six-week treatment of PPARδagonist ***501516 treatment significantly altered levels of metabolites,such as branched-chain amino acids(BCAAs),BCAA metabolites(3-hydroxyisobutyric acid and 3-hydroxyisovaleric acid),long-chain fatty acids,uric acid and ketone bodies(3-hydroxybutyric acid and 2-hydroxybutyric acid)which are all associated with the impaired systemic insulin *** pre sent results indicate the beneficial effect of PPARδagonist in alleviating insulin resistance of diabetic mice by favorably modulating metabolic profile,thus providing valuable information in understanding the therapeutic potential of PPARδagonists in correcting metabolic dysfunction in diabetes.

关键词： PPARδ BCAAs BCAA metabolites metabolomics Diabetic mice

Elucidating the interaction of kansui and licorice by comparative plasma/tissue metabolomics and a heatmap with relative fold change

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Journal of Pharmaceutical Analysis 2019年第5期9卷 312-323页

作者： Yan-Yan Chen Juan Shen Yu-Ping Tang Jin-Gao Yu Jing Wang Shi-Jun Yue Jie Yang Jia-Qian Chen Li-Mei Feng Zhen-Hua Zhu Wei-Wei Tao Li Zhang Jin-Ao Duan Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility State Key Laboratory of Research&Development of Characteristic Qin Medicine Resources(Cultivation)Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Researchand Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources IndustrializationShaanxi University of Chinese MedicineXi’an 712046Shaanxi ProvinceChina Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicineand Jiangsu Key Laboratory for High Technology Research of TCM FormulaeNanjing University of Chinese MedicineNanjing 210023Jiangsu ProvinceChina

Although compatibility is highly advocated in traditional Chinese medicine (TCM), inappropriate combination of some herbs may reduce the therapeutic action and even produce toxic effects. Kansui and licorice, one of TCM “Eighteen Incompatible Medicaments”, are the most representative cases of improper herbal combination, which may still be applied simultaneously under given conditions. However, the potential mechanism of their compatibility and incompatibility is unclear. In the present study, two different ratios of kansui and licorice, representing their compatibility and incompatibility respectively, were designed to elucidate their interaction by comparative plasma/tissue metabolomics and a heatmap with relative fold change. As a result, glycocholic acid, prostaglandin F2a, dihydroceramide and sphinganine were screened out as the principal alternative biomarkers of compatibility group;sphinganine, dihydroceramide, arachidonic acid, leukotriene B4, acetoacetic acid and linoleic acid were those of incompatibility group. Based on the values of biomarkers in each tissue, the liver was identified as the compatible target organ, while the heart, liver, and kidney were the incompatible target organs. Furthermore, important pathways for compatibility and incompatibility were also constructed. These results help us to better understand and utilize the two herbs, and the study was the first to reveal some innate characters of herbs related to TCM “Eighteen Incompatible Medicaments”.

关键词： Comparative metabolomics Kansui Licorice Incompatibility Heatmap

Hepatic metabolomics and transcriptomics to study susceptibility to ketosis in response to prepartal nutritional management

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Journal of Animal Science and Biotechnology 2020年第1期11卷 194-210页

作者： Khuram Shahzad Vincenzo Lopreiato Yusheng Liang Erminio Trevisi Johan S.Osorio Chuang Xu Juan J.Loor COMSATS Institute of Information Technology ChakShahzadIslamabad 44000Pakistan Department of Animal Sciences and Division of Nutritional Sciences University of IllinoisUrbanaIL 61801USA Istituto di Zootecnica Facoltàdi Scienze AgrarieAlimentari e AmbientaliUniversitàCattolica del Sacro Cuore29122 PiacenzaItaly Department of Dairy Science South Dakota State UniversityBrookingsSD 57006USA College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural UniversityXinyang Rd.5Daqing 163319China.

Background:Ketosis in dairy cows is associated with body fat mobilization during the peripartal ***-clinical and clinical ketosis arise more frequently in cows that are overfed energy during the entire dry(last 50 to 45 days prior to parturition)or close-up period(last^28 days prepartum).Methods:A retrospective analysis was performed on 12 cows from a larger cohort that were fed a higher-energy diet[1.54 Mcal/kg of dry matter(DM);35.9%of DM corn silage and 13%of DM ground corn]during the close-up dry period,of which 6 did not develop clinical ketosis(OVE,0.83 mmol/L plasma hydroxybutyrate;BHB)and 6 were diagnosed with clinical ketosis(KET,1.4 mmol/L BHB)during the first week postpartum.A whole-transcriptome bovine microarray(Agilent Technologies)and metabolomics(GC-MS,LC-MS;Metabolon~?Inc.)were used to perform transcript and metabolite profiling of liver tissue harvested at-10 days relative to parturition which allowed to establish potential associations between prepartal transcriptome/metabolome profiles and susceptibility to clinical ketosis ***:Cows in KET had greater(P=0.01)overall body weight between-2 and 1 week around parturition,but similar body condition score than *** dry matter intake(DMI)did not differ prepartum,KET cows had lower(P<0.01)DMI and similar milk yield as OVE cows during the first week *** analysis revealed a total of 3065 differentially expressed genes(DEG;P≤0.05)in *** identified 15 out of 313 total biochemical compounds significantly affected(P≤0.10)in *** those,greater concentrations(P≤0.06,+2.3-fold)of glycochenodeoxycholate in KET cows also have been detected in humans developing non-alcoholic fatty liver *** analysis using the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway database and the DEG revealed that,among the top 20 most-impacted metabolic pathway categories in KET,65%were overall *** included’Metabolism of cofactors

关键词： Bioinformatics Ketosis metabolomics Transition cow

1^H NMR-based metabolomics approach to investigating the renal protective effects of Genipin in diabetic rats

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Chinese Journal of Natural Medicines 2018年第4期16卷 261-270页

作者： TIAN Jun-Sheng ZHAO Lei SHEN Xiao-Li LIU Huan QIN Xue-Mei Modern Research Center for Traditional Chinese Medicine Shanxi UniversityTaiyuan 030006China College of Chemistry and Chemical Engineering of Shanxi University Taiyuan 030006China

Diabetic nephropathy is one of the various complications of diabetes mellitus, affecting patients for lifetime. Earlier studies have revealed that genipin can not only improve diabetes, but also induce cytotoxicity. Therefore, it is not clear which effect of genipin on kidneys occurs, when it is used in the treatment of diabetes. In the present study, we performed nuclear magnetic resonance(NMR)-based metabolomics analysis of urine and kidney tissue samples obtained from diabetic rats to explore the change of endogenous metabolites associated with diabetes and concomitant kidney disease. Nine significant differential metabolites that were closely related to renal function were screened. They were mainly related to three metabolic pathways: synthesis and degradation of ketone bodies, glycine, serine and threonine metabolism, and butanoate metabolism, which are involved in methylamine metabolism, energy metabolism and amino acid metabolism. In addition, after the intervention of genipin, the metabolic levels of all the metabolites tended to be normal, indicating a protective effect of genipin on kidneys. Our results may be helpful for understanding the antidiabetic effect of genipin.

关键词： Diabetes Diabetic nephropathy Genipin Protection metabolomics

UPLC/ESI-QTOF-MS-based metabolomics survey on the toxicity of triptolide and detoxication of licorice

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Chinese Journal of Natural Medicines 2017年第6期15卷 474-480页

作者： WANG Zhuo LIU Jian-Qun XU Jin-Di ZHU He KONG Ming ZHANG Guo-Hua DUAN Su-Min LI Xiu-Yang LI Guang-Fu LIU Li-Fang LI Song-Lin Department of Pharmaceutical Analysis and metabolomics Jiangsu Province Academy of Traditional Chinese Medicine Nan-jing 210028 China State Key Laboratory of Natural Medicines Department of Chinese Medicines Analysis China Pharmaceutical University Nan-jing 210009 China Key Laboratory of Modern Preparation of Traditional Chinese Medicines Ministry of Education Jiangxi University of Tradi-tional Chinese Medicine Nanchang 330004 China Department of Surgery Medical University of South Carolina Charleston SC 29466 USA

Triptolide(TP) from Tripterygium wilfordii has been demonstrated to possess anti-inflammatory, immunosuppressive, and anticancer activities. TP is specially used for the treatment of awkward rheumatoid arthritis, but its clinical application is confined by intense side effects. It is reported that licorice can obviously reduce the toxicity of TP, but the detailed mechanisms involved have not been comprehensively investigated. The current study aimed to explore metabolomics characteristics of the toxic reaction induced by TP and the intervention effect of licorice water extraction(LWE) against such toxicity. Obtained urine samples from control, TP and TP + LWE treated rats were analyzed by UPLC/ESI-QTOF-MS. The metabolic profiles of the control and the TP group were well differentiated by the principal component analysis and orthogonal partial least squares-discriminant analysis. The toxicity of TP was demonstrated to be evolving along with the exposure time of TP. Eight potential biomarkers related to TP toxicity were successfully identified in urine samples. Furthermore, LWE treatment could attenuate the change in six of the eight identified biomarkers. Functional pathway analysis revealed that the alterations in these metabolites were associated with tryptophan, pantothenic acid, and porphyrin metabolism. Therefore, it was concluded that LWE demonstrated interventional effects on TP toxicity through regulation of tryptophan, pantothenic acid, and porphyrin metabolism pathways, which provided novel insights into the possible mechanisms of TP toxicity as well as the potential therapeutic effects of LWE against such toxicity.

关键词： metabolomics Triptolide Licorice Toxicity Detoxication UPLC/ESI-QTOF-MS

High-throughput metabolomics reveals the perturbed metabolic pathways and biomarkers of Yang Huang syndrome as potential targets for evaluating the therapeutic effects and mechanism of geniposide

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Frontiers of Medicine 2020年第5期14卷 651-663页

作者： Heng Fang Aihua Zhang Xiaohang Zhou Jingbo Yu Qi Song Xijun Wang National Chinmedomics Research Center Sino-America Chinmedomics Technology Collaboration CenterNational TCM Key Laboratory of Serum PharmacochemistryLaboratory of MetabolomicsDepartment of Pharmaceutical AnalysisHeilongjiang University of Chinese MedicineHarbin 150040China State Key Laboratory of Quality Research in Chinese Medicine Macao University of Science and TechnologyAvenida Wai LongTaipaMacao 519020China

High-throughput metabolomics can clarify the underlying molecular mechanism of diseases via the qualitative and quantitative analysis of *** study used the established Yang Huang syndrome(YHS)mouse model to evaluate the efficacy of geniposide(GEN).Urine metabolic data were quantified by ultraperformance liquid chromatography-tandem mass *** non-target screening of the massive biological information dataset was performed,and a total of 33 metabolites,including tyramine glucuronide,aurine,and L-cysteine,were identified relating to *** differential metabolites directly participated in the disturbance of phase I reaction and hydrophilic transformation of ***,they were completely reversed by ***,as the auxiliary technical means,we also focused on the molecular prediction and docking results in network pharmacological and integrated analysis *** used integrated analysis to communicate the multiple results of metabolomics and network *** study is the first to report that GEN indirectly regulates the metabolite“tyramine glucuronide”through its direct effect on the target heme oxygenase 1 in ***,heme oxygenase-1,a prediction of network pharmacology,was the confirmed metabolic enzyme of phase I reaction in *** study indicated that the combination of high-throughput metabolomics and network pharmacology is a robust combination for deciphering the pathogenesis of the traditional Chinese medicine(TCM)syndrome.

关键词： metabolomics liquid chromatography-mass spectrometry metabolites metabolic pathways

Effect of compound Sophorae decoction in the treatment of ulcerative colitis by tissue extract metabolomics approach

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Journal of Traditional Chinese Medicine 2021年第3期41卷 414-423页

作者： HONG Zongchao CAI Quan DUAN Xueyun YANG Yanfang WU Hezhen JIANG Nan FAN Heng College of Pharmacy Hubei University of Chinese MedicineWuhan 430065China Department of Integrated Traditional Chinese and Western Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Hubei Provincial Hospital of Traditional Chinese Medicine Wuhan430061China Hubei Province Academy of Traditional Chinese Medicine Wuhan430074China

OBJECTIVE: To investigate how compound Sophorae decoction(CSD) works on rats' models of ulcerative colitis(UC) induced by 2,4,6-trinitrobenzenesulfonic acid solution(TNBS) by metabolomics studies of colon, liver, and kidney tissue ***: Rats with UC induced by TNBS enema were used as models in this study. Metabolic profiles of the three tissues were analyzed and pathway analysis of biomarkers was performed after CSD administration and further integration of metabolic ***: Thirteen biomarkers were screened from colon, liver, and kidney tissue extracts, and the levels of these substances were up-or down-regulated in the model group, but their levels were reversed after CSD administration. These biomarkers were mainly related to Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Glutathione metabolism, Arachidonic acid metabolism, Nicotinate and nicotinamide metabolism,Alanine, aspartate and glutamate ***: CSD could significantly ameliorate the symptoms of UC by regulating multiple metabolic pathways.

关键词： colitis ulcerative metabolomics trinitrobenzenesulfonic acid compound Sophorae decoction