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metabolic profiling of endogenous bile acids:a novel method to assess hepatoprotective effect of Tanreqing capsule on carbon-tetrachloride-induced liver injury in rats

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Chinese Journal of Natural Medicines 2018年第4期16卷 271-283页

作者： XU Ying ZHONG Ping-Ping TAO Yan-Yan Shanghai Kaibao Pharmaceutical Co. Ltd.Shanghai 201401China School of Pharmacy Shanghai University of Traditional Chinese MedicineShanghai 201203China Institute of Liver Diseases Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai201203China Shanghai Clinical Key Laboratory of Traditional Chinese Medicine Shanghai 201203China

Tanreqing(TRQ), a traditional Chinese medicine(TCM) formula, can alleviate liver injury and improve liver function. Its pharmacological mechanisms of actions are still unclear due to its complex components and multi-target natures. Metabolomic study is an effective approach to investigating drug pharmacological actions, new diagnostic markers, and potential mechanisms of actions. In the present study, a new strategy was used to evaluate the protective effect of TRQ capsule against carbon tetrachloride(CCl_4)-induced hepatotoxicity in rats, by analyzing metabolic profiling of endogenous bile acids(BAs) along with biochemical and histological analyses. BAs concentrations were determined by ultra-performance liquid chromatography coupled with quadrupole mass spectrometry(UPLC-MS). Principal component analysis and partial least squares discriminant analysis were then employed to analyze the UPLC-MS results and compare the hepatoprotective effect of TRQ capsule in different groups at the doses of 0.36, 1.44, and 2.88 g·kg^(-1) body weight, respectively. Moreover, our results suggested that taurocholic acid(TCA) and taurohyodesoxycholic acid(THDCA) were the most important biochemical markers, which were indicative of CCl_4-induced acute hepatic damage and hepatoprotective effect of TRQ capsule. Therefore, this new strategy would be an excellent alternative method for evaluating hepatoprotective effect and proposing potential mechanisms of action for other drugs as well.

关键词： metabolic profiling Bile acids Hepatoprotective effect Tanreqing capsule UPLC-MS

Gas chromatography mass spectrometry based metabolic profiling reveals biomarkers involved in rice-gall midge interactions

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Journal of Integrative Plant Biology 2014年第9期56卷 837-848页

作者： Ruchi Agarrwal Jagadish Sanmallappa Bentur Suresh Nair International Centre for Genetic Engineering and Biotechnology(ICGEB) Directorate of Rice Reearch Agri Biotech Foundation

The Asian rice gall midge（Orseolia oryzae WoodMason） is a serious pest of rice that causes huge loss in *** feeding inside the susceptible host,maggots secrete substances that facilitate the formation of a hollow tube-like structure called gall and prevent panicle *** present investigation was carried out to get an account of biochemical changes occurring in the rice plant upon gall midge *** profiling of host tissues from three rice varieties,namely,TN1,Kavya,and RP2068,exposed to gall midge biotype 1（GMB1）,was carried out using gas chromatography mass spectrometry（GC-MS）.TN1 and GMB1 represented compatible interaction,while Kavya and GMB1 as well as RP2068 and GMB1 represented incompatible *** current study identified several metabolites that could be grouped as resistance,susceptibility,infestation,and host features based on their relative *** may be regarded as biomarkers for insect-plant interaction in general and rice-gall midge interaction in particular.

关键词： Biomarkers gas chromatography mass spectrometry insect-plant interaction metabolic profiling metabolites rice-gall midge interaction

Shining the spotlight on NMR metabolic profiling and bioactivities of different solvent extracts of Piliostigma thonningii

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Food Bioscience 2022年第3期47卷 1337-1350页

作者： Kouadio Ibrahime Sinan Gokhan Zengin Antonio Fiorentino Chiara Vuotto Mohamad Fawzi Mahomoodally Sharmeen Jugreet Jasmina Glamočlija Marina Soković Ouattara Katinan Etienne Catarina Guerreiro Pereira Luísa Custódio Kannan R.R.Rengasamy Brigida D'Abrosca Department of Biology Science FacultySelcuk UniversityKonyaTurkey Dipartimento di Scienze e Tecnologie Ambientali Biologiche e Farmaceutiche –DiSTABiF Università degli Studi della Campania “Luigi Vanvitelli”via Vivaldi 43 I-81100CasertaItaly Department of Health Sciences Faculty of Medicine and Health SciencesUniversity of MauritiusRéduit80837Mauritius Laboratory of Mycology Department of Plant PhysiologyInstitute for Biological Research “Siniša Stanković”National Institute of Republic of SerbiaUniversity of BelgradeBelgradeSerbia Laboratoire de Botanique UFR BiosciencesUniversité Félix Houphouët-BoignyAbidjanCote d'Ivoire Centre of Marine Sciences University of AlgarveCampus de Gambelas8005-139FaroPortugal Centre for Transdisciplinary Research Department of PharmacologySaveetha Dental CollegeSaveetha Institute of Medical and Technical Sciences (SIMATS)Chennai600077India

Piliostigma thonningii (Fabaceae) is a versatile medicinal plant used as a traditional remedy,especially in African *** the present study,ethyl acetate,methanolic and water extracts of different parts of (fruits,leaves,stem barks) *** were evaluated for their bioactive contents,enzyme inhibitory,antioxidant and antimicrobial *** potentials were also displayed in various methods like phosphomolybdenum assay (0.79–5.94 mmol TE/g),radical scavenging assays (DPPH:20.39–471.28 mg TE/g;ABTS:42.43–654.14 mg TE/g);reducing assays (CUPRAC:102.98–1207.42 mg TE/g;FRAP:45.30–656.25 mg TE/g) and metal chelating activity (2.55–22.32 mg EDTAE/g).In particular,the methanolic and water extracts showed better antioxidant *** for a few,the extracts were also found to act as inhibitors of enzymes such as acetylcholinesterase (2.35–5.35 mg GALAE/g),butyrylcholinesterase (0.83–13.80 mg GALAE/g),tyrosinase (4.03–143.86 mg KAE/g),amylase (0.21–1.39 mmol ACAE/g) and glucosidase (3.52–7.13 mmol ACAE/g).Additionally,all the tested extracts possessed significant antibacterial (minimum inhibitory concentration (MIC) and minimum bactericidal concentration:0.11–0.61 mg/ml) and antifungal (MIC and minimum fungicidal concentration:0.04–0.61 mg/ml) ***,the data garnered from the present study provides a bright scope for the advanced investigation on this particular plant possessing pharmacologically active traits.

关键词： Piliostigma thonningii Bioactivities Enzyme inhibitors Antioxidants Antimicrobial NMR metabolic profiling

GC/MS-based differential metabolic profiling of human peptic ulcer disease to study Helicobacter pylori-induced metabolic perturbations

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BIOCELL 2021年第5期45卷 1299-1311页

作者： GAGANJOT GUPTA DEEPAK BANSAL ANSHULA SHARMA TAWSEEF AHMAD ATUL SACHDEV AJAZ AHMAD HAMED A.EL-SEREHY BALJINDER KAUR Department of Biotechnology Punjabi UniversityPatiala147002India Department of General Medicine Government Medical College and HospitalChandigarh160047India Department of Clinical Pharmacy College of PharmacyKing Saud UniversityRiyadh11451Saudi Arabia Department of Zoology College of ScienceKing Saud UniversityRiyadh11451Saudi Arabia

Helicobacter pylori infection has been significantly linked to Peptic Ulcer Disease and Gastric *** fingerprinting may offer a principal way of early diagnosis and to understand the molecular mechanism of ***-induced *** rationale of the study is to explore the underlying distinct metabolic mechanisms of ***-induced PUD and to identify potential biomarkers for disease diagnosis and associated risks using Gas chromatography/mass ***/MS-based analytical method was used to compare metabolic profiles of healthy controls(N=20)and peptic ulcer patients(N=45).Acquired metabolomic data were analyzed by constructing a diagnostic model using principal component analysis and a non-parametric two-tailed paired Wilcoxon analysis to identify disease-specific metabolic biomarkers.A total of 75 low-molecular-weight endogenous metabolites were detected during comparative metabolomic analysis of PUD *** gut tissues,among which 16 metabolites are being proposed to be diagnostic markers of Human *** related to amino acids,carbohydrates,fatty acids,organic acids,and sterol metabolism were significantly revealed during this differential metabolomic *** convincingly suggest that metabolic profiles can contribute immensely in early diagnosis of the disease and understanding molecular mechanisms of disease progression for predicting novel drug targets for prophylactic and anaphylactic measures.

关键词： Peptic ulcer disease Gastric cancer Helicobacter pylori metabolic profiling Perturbations Stomach ulcers

Cerebral metabolic profiling of Hypothermic Circulatory Arrest with and Without Antegrade Selective Cerebral Perfusion： Evidence from Nontargeted Tissue Metabolomics in a Rabbit Model

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Chinese Medical Journal 2016年第6期129卷 702-708页

作者： Li-Hua Zou Jin-Ping Liu Hao Zhang Shu-Bin Wu Bing-Yang Ji Department of Cardiopulmonary Bypass Fuwai Hospital and National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100037 China Department of Surgery and Center for Pediatric Cardiac Surgery Fuwai Hospital and National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100037 China

Background： Antegrade selective cerebral perfusion （ASCP） is regarded to perform cerebral protection during the thoracic aorta surgery as an adjunctive technique to deep hypothermic circulatory arrest （DHCA）. However, brain metabolism profile after ASCP has not been systematically investigated by metabolomics technology. Methods： To clarify the metabolomics profiling of ASCP, 12 New Zealand white rabbits were randomly assigned into 60 min DHCA with （DHCA＋ASCP [DA] group, n = 6） and without （ DHCA [D] group, n = 6） ASCP according to the random number table. ASCP was conducted by cannulation on the right subclavian artery and cross-clamping of the innominate artery. Rabbits were sacrificed 60 min after weaning off cardiopulmonary bypass. The metabolic features of the cerebral cortex were analyzed by a nontargeted metabolic profiling strategy based on gas chromatography-mass spectrometry. Variable importance projection values exceeding 1.0 were selected as potentially changed metabolites, and then Student＇s t-test was applied to test for statistical significance between the two groups. Results： metabolic profiling of brain was distinctive significantly between the two groups （Q2y = 0.88 for partial least squares-DA model）. In comparing to group D, 62 definable metabolites were varied significantly after ASCP, which were mainly related to amino acid metabolism, carbohydrate metabolism, and lipid metabolism. Kyoto Encyclopedia of Genes and Genomes analysis revealed that metabolic pathways after DHCA with ASCP were mainly involved in the activated glycolytic pathway, subdued anaerobic metabolism, and oxidative stress. In addition, L-kynurenine （P = 0.0019）, 5-methoxyindole-3-acetic acid （P = 0.0499）, and 5-hydroxyindole-3-acetic acid （P = 0.0495） in tryptophan metabolism pathways were decreased, and citrulline （P - 0.0158） in urea cycle was increased in group DA comparing to group D. Conclusions： The present study applied metabolomics analysis to identify the cerebral met

关键词： Antegrade Selective Cerebral Perfusion Cardiopulmonary Bypass metabolic profiling Metabolomics

Clonal variation in growth,PSII photochemical activity and polar metabolites in Pinus elliottii×***

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Journal of Forestry Research 2022年第1期33卷 63-74页

作者： Wenbing Guo Fencheng Zhao Yang Liu Zhen Li Huishan Wu Yiliang Li Fangyan Liao Guangdong Provincial Key Laboratory of Silviculture Protection and UtilizationGuangdong Academy of Forestry.Address:No.233 Guangshan First RoadTianhe DistrictGuangzhou 510520People’s Republic of China

Pinus elliottii×*** is a widely planted commercial tree hybrid in South *** investigate the potential of physiological parameters for clonal selection,cuttings of three clones(EH3,EH4 and EH5)and a control were grown in phosphorus treated *** variations to phosphorous,photosystem II activity and polar metabolite abundance in needles were noted among *** EH5 was the most P-responsive and had maximum height *** highest maximum quantum yield of PSII in the dark(F_(v)/F_(m)),maximum quantum yield of PSII in the light(F_(v)'/F_(m)')and the actual PSII efficiency(Φ_(PSII))values were also found in EH5.A total of 64 polar metabolites were identified,21 were regulated by phosphorous,while 28 accumulated differentially among the three *** were few overlapping responsive metabolites to phosphorous *** EH5,the abundance of maltotriitol,neohesperidin and raffinose were highest,whereas galactinol and shikimic acid were lower compared with EH3 and *** results reveal that physiological traits were genetically controlled and might be useful for selecting clones with high phosphorous utilization efficiency.

关键词： Carbohydrates Chlorophyll fuorescence parameters metabolic profiling Phosphorus fertilization Physiological traits

A Genome-Wide Scenario of Terpene Pathways in Self-pollinated Artemisia annua

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Molecular Plant 2015年第11期8卷 1580-1598页

作者： Dong-Ming Ma Zhilong Wang Liangjiang Wang Fatima Alejos-Gonzales1 Ming-An Sun De-Yu Xie Department of Plant and Microbial Biology North Carolina State University Raleigh NC 27695 USA Department of Genetics and Biochemistry Clemson University Clemson SC 29634 USA Virginia Bioinformatics Institute Virginia Polytechnic Institute and State University Blacksburg VA 24061 USA Present address: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization Hunan Agricultural University Changsha Hunan 410128 China

Scenarios of genes to metabolites in Artemisia annua remain uninvestigated. Here, we report the use of an integrated approach combining metabolomics, transcriptomics, and gene function analyses to charac- terize gene-to-terpene and terpene pathway scenarios in a self-pollinating variety of this species. Eightyeight metabolites including 22 sesquiterpenes （e.g., artemisinin）, 26 monoterpenes, two triterpenes, one diterpene and 38 other non-polar metabolites were identified from 14 tissues. These metabolites were differentially produced by leaves and flowers at lower to higher positions. Sequences from cDNA libraries of six tissues were assembled into 18 871 contigs and genome-wide gene expression profiles in tissues were strongly associated with developmental stages and spatial specificities. Sequence mining identified 47 genes that mapped to the artemisinin, non-amorphadiene sesquiterpene, monoterpene, triterpene, 2-C- methyl-D-erythritol 4-phosphate and mevalonate pathways. Pearson correlation analysis resulted in network integration that characterized significant correlations of gene-to-gene expression patterns and gene expression-to-metabolite levels in six tissues simultaneously. More importantly, manipulations of amorpha-4,11-diene synthase gene expression not only affected the activity of this pathway toward artemisinin, artemisinic acid, and arteannuin b but also altered non-amorphadiene sesquiterpene and genome-wide volatile profiles. Such gene-to-terpene landscapes associated with different tissues are fundamental to the metabolic engineering of artemisinin.

关键词： Artemisia annua artemisinin terpene metabolic profiling pyrosequencing

Comprehensive profiling and natural variation of flavonoids in rice

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Journal of Integrative Plant Biology 2014年第9期56卷 876-886页

作者： Xuekui Dong Wei Chen Wensheng Wang Hongyan Zhang Xianqing Liu Jie Luo National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural University Key Laboratory of Horticultural Plant Biology(Ministry of Education) Huazhong Agricultural University College of Life Science and Technology Huazhong Agricultural University

Flavonoids constitute a major group of plant phenolic *** extensively studied in Arabidopsis,profiling and naturally occurring variation of these compounds in rice（Oryza sativa）,the monocot model plant,are less *** a collection of rice germplasm,comprehensive profiling and natural variation of flavonoids were presented in this *** of a widely targeted metabolomics method facilitated the simultaneous identification and quantification of more than 90 flavonoids using liquid chromatography tandem mass spectrometry（LC-MS/MS）.Comparing flavonoid contents in various tissues during different developmental stages revealed tissue-specific accumulation of most *** investigation indicated that flavone mono-C-glycosides,malonylated flavonoid O-hexosides,and some flavonoid O-glycosides accumulated at significantly higher levels in indica than in japonica,while the opposite was observed for aromatic acylated flavone *** contrast to the highly differential accumulation between the two subspecies,relatively small variations within subspecies were detected for most ***,an association analysis between flavonoid accumulation and its biosynthetic gene sequence polymorphisms disclosed that natural variation of flavonoids was probably caused by sequence polymorphisms in the coding region of flavonoid biosynthetic *** work paves the way for future dissection of biosynthesis and regulation of flavonoid pathway in rice.

关键词： Flavonoids metabolic profiling natural variation Oryza sativa single-nucleotide polymorphisms

metabolic and physiological regulation of Chlorella sp.(Trebouxiophyceae, Chlorophyta) under nitrogen deprivation

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Journal of Oceanology and Limnology 2019年第1期37卷 186-198页

作者： YONG Wai-Kuan LIM Phaik-Eem VELLO Vejeysri SIM Kae-Shin ABDUL MAJID Nazia MUSTAFA Emienour Muzalina NIK SULAIMAN Nik Meriam LIEW Kan-Ern CHEN Brenna Jia-Tian PHANG Siew-Moi Institute of Ocean and Earth Sciences University of Malaya Institute of Graduate Studies University of Malaya Institute of Biological Sciences Faculty of Science University of Malaya School of Fisheries and Aquaculture Sciences University of Malaysia Department of Chemical Engineering Faculty of Engineering University of Malaya Airbus Group Malaysia Menara HLA Aerospace Malaysia Innovation Centre

A freshwater green microalgae Chlorella sp., UMACC344 was shown to produce high lipid content and has the potential to be used as feedstock for biofuel production. In this study, photosynthetic effciency, biochemical pro?les and non-targeted metabolic pro?ling were studied to compare between the nitrogen-replete and deplete conditions. Slowed growth, change in photosynthetic pigments and lowered photosynthetic effciency were observed in response to nitrogen deprivation. Biochemical pro?les of the cultures showed an increased level of carbohydrate, lipids and total fatty acids, while the total soluble protein content was lowered. A trend of fatty acid saturation was observed in the nitrogen-deplete culture with an increase in the level of saturated fatty acids especially C16:0 and C18:0, accompanied by a decrease in proportions of monounsaturated and polyunsaturated fatty acids. Fifty-nine metabolites, including amino acids, lipids, phytochemical compounds, vitamins and cofactors were signi?cantly dysregulated and annotated in this study. Pathway mapping analysis revealed a rewiring of metabolic pathways in the cells, particularly purine, carotenoid, nicotinate and nicotinamide, and amino acid metabolisms. Within the treatment period of nitrogen deprivation, the key processes involved were reshu ? ing of nitrogen from proteins and photosynthetic machinery, together with carbon repartitioning in carbohydrates and lipids.

关键词： metabolic profiling Chlorella sp. nitrogen stress lipid fatty acid