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Purification and characterization of a novel immunomodulatory protein from the medicinal mushroom Trametes versicolor

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Science China(Life Sciences) 2011年第4期54卷 379-385页

作者： LI Feng WEN HuaAn ZHANG YongJie AN Min LIU XingZhong Key Laboratory of Systematic Mycology and Lichenology Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China Graduate University of Chinese Academy of Sciences Beijing 100049 China School of Life Sciences Shanxi University Taiyuan 030006 China

Bioactive proteins represent an important group of functional agents in medicinal *** versicolor(L.) Lloyd is a mushroom frequently used in traditional Chinese medicine for its anti-tumor and immunomodulatory activities.A new immunomodulatory protein from ***,named TVC,was purified by ammonium sulfate precipitation,ion-exchange chromatography and gel filtration *** dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the purified protein revealed a single band with a molecular weight of 15.0 *** polyacrylamide gel analysis revealed a band at 30 kD,indicating that TVC exists in solution as a *** focusing showed that TVC was an acidic protein with an isoelectric point of *** was found to lack carbohydrate modifications(based on periodic acid/Schiff staining) and it does not agglutinate mouse red blood cells,suggesting that TVC is not a lectin-like *** activity assays dem-onstrated that TVC can enhance the proliferation of splenocytes,while it has no stimulatory effects on CD4+and CD8+T cells. TVC markedly increases the proliferation of human peripheral blood lymphocytes in a dose-dependent manner and enhances the production of both nitric oxide and tumor necrosis factor-alpha by lipopolysaccharide-induced murine *** results indicate that TVC is an immunostimulant that can boost immune *** of the N-terminal amino acid residues and mass spectrometry results with the protein database revealed no homologous proteins.

关键词： biological activity immunomodulatory protein medicinal mushroom purification Trametes versicolor

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A novel fibrinolytic enzyme from Cordyceps militaris, a Chinese traditional medicinal mushroom

A novel fibrinolytic enzyme from Cordyceps militaris, a Chin...

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全国家(柞)蚕资源高值化利用学术研讨会

作者： Li Cui 董明盛 Xiaohong Chen Mei Jiang Xin Lv Guijun Yan College of Food Science and Technology Nanjing Agricultural University 南京农业大学食品科技学院

A novel fibrinolytic enzyme from Cordyceps militaris was purified and partially characterized for the first time, which was designated C. Militaris fibrinolytic enzyme（CMase）. This extracellular enzyme from C. militaris was isolated by ammonium sulphate fraction, and purified to electrophoretic homogeneity using gel filtration chromatography. The apparent molecular mass of the purified enzyme was estimated to be 27.3 kDa by SDS-PAGE. The optimum pH and temperature for the enzyme activity were pH 6.0 and 25℃, respectively. In the presence of metal ions such as Mg2+and Fe2+ions the activity of the enzyme increased, whereas EDTA and Cu2+ ion inhibited the enzyme activity. Interestingly the N-terminal amino acid sequences of the enzyme is extremely similar to those of the trypsin proteinases from insects, and has no significant homology with those of the fibrinolytic enzyme from other medicinal mushroom. In conclusion, C. militaris produces a strong fibrinolytic enzyme CMase and may be considered as a new source for thrombolytic agents

关键词： Cordyceps militaris CMase Entomogenous fungus Fibrinolytic activity medicinal mushroom

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Cloning and characterization of a squalene synthase gene from a medicinal mushroom, Inonotus obliquus(Fr.) Pilat

Cloning and characterization of a squalene synthase gene fro...

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中国生物工程学会2014年学术年会暨全国生物技术大会

作者：曹小迎江苏师范大学江苏省药用植物生物技术重点实验室

Squalene synthase catalyzes the condensation of two molecules of farnesyl diphosphate to produce squalene, the first committed precusor for sterol, brassinosteroid, and triterpene biosynthesis. A squalene synthase gene, designated Io SQS, was isolated from Inonotus obliquus, a medicinal mushroom produces a plethora of bioactive triterpenes. The Io SQS cDNA was found to contain an open reading frame of 1,476 bp, encoding a protein of 491 amino acids with a calculated molecular mass of 55.85 kDa. The Io SQS genomic DNA sequence consisted of 1,813 bp and contained four exons and three introns. The restriction fragment polymorphisms revealed by Southern blot analysis suggested that IoSQS was a single copy gene. Promoter analysis indicated that the 5’ upstream region of IoSQS possessed various potential elements associated with physiological and environmental factors. The expression pattern of IoSQS in different stages and under MeJA treatment were correlative with the accumulation of total triterpenoids, and was consistent with the predicted results of Io SQS promoter region. The N-terminal 466 residues of the hydrophilic sequence was expressed as a His-tagged protein in E. coli, and the resultant bacterial crude extract was incubated with farnesyl diphosphate and NADPH, squalene was detected in vitro reaction mixture by HPLC analysis. These results suggest that IoSQS enzyme is involved in squalene production in I. obliquus.

关键词： Squalene synthase gene Inonotus obliquus medicinal mushroom Promoter Methyl jasmonate Total triterpenoids

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Identification of Common Edible and medicinal mushrooms by DNA Barcoding

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World Journal of Traditional Chinese Medicine 2016年第3期2卷 10-16页

作者： Yan-Yan Su Huan Tang Xi-Wen Li Wei Sun Zeng-Hua Chen Li Xiang Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences Huaibei Normal University Beijing Qianjunfang Academy of Sciences

Objective:To identify common edible and medicinal mushrooms using the internal transcribed spacer(ITS) ***:A total of eighty-five samples belonging to forty-three species were used in this *** regions were amplified and *** ITS regions were analyzed using BLAST and MEGA 5.10 ***:Forty species were identified correctly via BLAST method whereas forty-one species were distinguished via Neighbor joining(NJ)*** the species could be distinguished by BLAST combined with NJ tree and ITS sequence ***:Forty-three common species of edible and medicinal mushrooms were effectively identified using DNA barcoding technology based on ITS region.

关键词： Edible mushroom medicinal mushroom ITS DNA Barcoding

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Morphology, Phylogeny and Culture Characteristics of Ganoderma gibbosum Collected from Kunming, Yunnan Province, China

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Phyton-International Journal of Experimental Botany 2020年第3期89卷 743-764页

作者： Thatsanee Luangharn Samantha C.Karunarathna Peter E.Mortimer Kevin D.Hyde Jianchu Xu Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming650201China University of Chinese Academy of Sciences Beijing100049China East and Central Asia Regional Office World Agroforestry Centre(ICRAF)Kunming650201China Centre for Mountain Futures Kunming Institute of BotanyKunming650201China Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai57100Thailand

Ganoderma is a genus of medicinally and economically important mushrooms in the family *** species are popular medicinal mushrooms and their health benefits are *** is a cosmopolitan genus that is widely distributed in both tropical and temperate *** genus is characterized by its unique laccate or non-laccate species with double-walled ***,we report on eight collections of *** collected during surveys in Kunming,Yunnan Province,*** specimens are described and illustrated based on macro-and micro-morphological *** DNA of the eight *** strains were extracted using the Biospin Fungal Extraction Kit following manufacturer *** of the Internal Transcribed Spacer(nrITS)region was carried out using ITS5/ITS4 primers and LROR/LR5 for the nuclear ribosomal large subunit 28S rDNA gene(LSU).Phylogenetic analysis with closely related species to *** showed that all eight collections grouped with *** with 100%bootstrap *** similarity and morphological variations within the eight collections of *** are discussed.

关键词： Chinese medicine medicinal mushroom morphology phylogeny

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“Ganoderma lucidum sensu lato”-a sacred mushroom for immortality

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Studies in Fungi 2020年第1期5卷 508-516页

作者： Ayesha P Maham A Ariza A Husnain H Faculty of Pharmacy University of LahoreLahorePakistan Faculty of Pharmacy University of Central PunjabLahorePakistan

Ganoderma lucidum sensu lato,is an ancient Chinese mushroom commonly known as“Reishi”or“Linghzi”which has been used for nutritional and therapeutic *** is a basidiomycete widely distributed in temperate and tropical *** 2000 classes of Reishi,only 6 types are commonly presented to be effective for therapeutic *** 6 types were known as black,red,blue,yellow,white and purple,*** constituents of Reishi are Polysaccharides,Triterpenoids,Sterols and *** has been clinically used as immune system modulator,antiviral,cytotoxic agent,antibacterial,hepatoprotective,anti-inflammatory and also in the treatment of neurological *** wide clinical scope of Reishi has influenced to name it in English with the nickname of“the mushroom of immortality”.

关键词： Linghzi medicinal mushroom Reishi Therapeutic value

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Research progress of polysaccharides from medicinal Fungi

Research progress of polysaccharides from Medicinal Fungi

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中国菌物学会2018年学术年会

作者： Jingsong Zhang Hua Fan Yan Yang Qingjiu Tang Yanfang Liu Wei Jia Wenhan Wang Chuanhong Tang Di Wu Shuai Zhou Jie Feng Haining Zhuang National Research Center of Edible Fungi Biotechnology and Engineering

The medicinal Fungi had played an important role in traditional Chinese medicine and have been used to treat different diseases in China for a long time. In recent years, many studies have been undertaken worldwide,and have ascertained that components of the mushroom possessed various therapeutic functions including immunomodulating activity, antitumor, antidiabetes and hepatoprotective ability and so on. It was found that many bioactivities of medicinal Fungi are relative with the polysaccharide. The study was focused on chemical structure and bioactivity of polysaccharides from 7 different important medicinal fungi, Ganoderma lucidum,Ganoderma sinense, Hericium erinaceus, Pleurotus eryngii,Coprinus comatus,Phellinus igniarius,Tremella aurantialba,Flammulina velutiper. 22 homogeneous polysaccharides were purified from hot water and alkali extracts of the fruiting body or spores using a series of chromatographic steps. Their chemical structures were elucidated by sugar analysis, methylation analysis and NMR. These polysaccharides was classified toβ-glucan,α-glucan, Fucogalactan, Fucoglucogalactan, Galactoglucan, acidic polysaccharides. Conformation of a 1,3/1,6-glucan from G. lucidum was studied by HPSEC-MALLS-RI system and Laser light scattering method;it was found conformation of the glucan is triple helix in solution. The bioactivity of these polysaccharides were found to induce macrophage activation and B or T cell activation, stimulate the proliferation of lymphocytes,stimulate the secretion of cytokines and immunoglobulins of lymphocytes and inhibit the Sarcoma S180 tumor growth. A sulfated polysaccharide deritive of Ganoderma lucidum, GLPss58, was found to possess the anti-inflammatory properties by inhibited the L-selectin – s Tyr/sLeX binding of lymphocytes. Our research results indicated that the structure and bioactivity of polysaccharides from medicinal fungi was diversity, it is difficult to obtain the clear structure-functional relationship of polysacc

关键词： medicinal mushroom glucan bioactivity

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