Identification of Common Edible and Medicinal Mushrooms by DNA Barcoding

作     者：Yan-Yan Su Huan Tang Xi-Wen Li Wei Sun Zeng-Hua Chen Li Xiang 

作者机构：Institute of Chinese Materia MedicaChina Academy of Chinese Medical Sciences Huaibei Normal University Beijing Qianjunfang Academy of Sciences 

出 版 物：《World Journal of Traditional Chinese Medicine》 (世界中医药杂志（英文）)

年 卷 期：2016年第2卷第3期

页      面：10-16页

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 10[医学] 100602[医学-中西医结合临床] 

基　　金：supported by the National Natural Science Foundation(81503192) the Major Scientific and Technological Special Project for "Significant New Drug Creation"(No.81503192) 

主　　题：Edible mushroom Medicinal mushroom ITS DNA Barcoding 

摘      要：Objective:To identify common edible and medicinal mushrooms using the internal transcribed spacer(ITS) region.Method:A total of eighty-five samples belonging to forty-three species were used in this study.ITS regions were amplified and sequenced.All ITS regions were analyzed using BLAST and MEGA 5.10 methods.Results:Forty species were identified correctly via BLAST method whereas forty-one species were distinguished via Neighbor joining(NJ)tree.All the species could be distinguished by BLAST combined with NJ tree and ITS sequence characteristics.Conclusion:Forty-three common species of edible and medicinal mushrooms were effectively identified using DNA barcoding technology based on ITS region.