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Molecular and morphological characterization of stunt nematodes of wheat, maize, and rice in the savannahs of northern Nigeria

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Journal of Integrative Agriculture 2022年第2期21卷 586-595页

作者： Sulaiman ABDULSALAM PENG Huan LIU Shi-ming HUANG Wen-kun KONG Ling-an PENG De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural SciencesBeijing 100193P.R.China Division of Agricultural Colleges Department of Crop ProtectionAhmadu Bello UniversityZaria 810107Nigeria

Stunt nematodes(Tylenchorhynchus spp.)are obligate migratory root ecto-parasitic nematodes found in the fields of many cultivated crops.These nematodes,with phyto-sanitary potential,are frequently ignored or misdiagnosed as pests,and this may pose a threat to food security.The accuracy of ITS identification based on a morphological approach has been challenged recently,due to the overlapping of the morphological and morphometric characters of the species.Consequently,the objective of this study is to identify and characterize stunt nematodes present in 54 fields cultivated with cereal crops(wheat,maize and rice)in the savannahs of northern Nigeria,using integrative taxonomy and molecular approaches.The molecular and morphological studies identified and confirmed the presence of T.annulatus as the occurring specie in the savannahs of northern Nigeria.The phylogenetic analysis was carried out using the internal transcribed spacer(ITS)and 28S genes of ribosomal DNA further confirmed the presence of T.annulatus.The first molecular characterization and sequences of the ITS and 28S rDNA gene for T.annulatus from Nigeria were provided by this research.Also,according to our literature search,this is the first report on T.annulatus in wheat,maize and rice in the savannahs of northern Nigeria.Further study to test the pathogenicity of the parasitic nematode species found in this survey is recommended for the prioritization and development of efficient management strategies.

关键词： plant-parasitic nematodes food security Tylenchorhynchus annulatus ITS phylogeny

基于16S rDNA和ITS技术研究酿酒酵母细胞壁对育肥牛肠道微生物的影响

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微生物学报 2024年

作者：王燕陈志龙施安李丹李博侯鹏霞张恩平西北农林科技大学动物科技学院宁夏农林科学院固原分院宁夏农林科学院动物科学研究所

【目的】本研究旨在通过16S rDNA和ITS测序技术探究酿酒酵母细胞壁对育肥牛肠道微生物的影响。【方法】选择体重550 kg左右西门塔尔杂交育肥牛40头，随机分为4组，每组10头牛，对照组饲喂基础饲粮，试验1、2、3组每日每头分别在基础饲... 详细信息

【目的】本研究旨在通过16S rDNA和ITS测序技术探究酿酒酵母细胞壁对育肥牛肠道微生物的影响。【方法】选择体重550 kg左右西门塔尔杂交育肥牛40头，随机分为4组，每组10头牛，对照组饲喂基础饲粮，试验1、2、3组每日每头分别在基础饲粮中添加酿酒酵母细胞壁5、10、15 g。试验预试期10 d，正试期94 d，试验结束前7天于晨饲前采集肠道粪便。【结果】16S rDNA分析结果显示：(1) 试验3组Chao和ACE指数显著高于其他组(PITS分析结果显示：(1) 各组间α、β多样性无显著差异(P>0.05)。(2) 门水平上，子囊菌门(Ascomycota)占比50%以上，是主要优势菌门；青霉属(Penicillium)、Unidentified＿Ascomycota＿sp.、曲霉属(Aspergillus)、Orpinomyces和Eurotium为主要优势菌属。(3) 经过LEfSe分析，共检测到8个差异物种(LDA≥3，P<0.05)，分别有3、3、2个差异物种在对照组、试验2组和试验3组发挥重要作用。【结论】本研究条件下，饲粮添加10-15 g/d酿酒酵母细胞壁提高了育肥牛肠道细菌群落的丰富度，显著增加了有益菌属Provetella＿9、Tolypocladium和Torulaspora的相对丰度，有利于优化肠道微生态环境。

关键词：酿酒酵母细胞壁育肥牛菌群结构 16S rDNA ITS

Space Division Multiple Access for Cellular V2X Communications

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Computers, Materials & Continua 2022年第10期73卷 1195-1206页

作者： Doaa Sami Khafaga Mohammad Zubair Khan Muhammad Awais Javed Amel Ali Alhussan Wael Said Department of Computer Sciences College of Computer and Information SciencesPrincess Nourah bint Abdulrahman UniversityP.O.Box 84428Riyadh11671Saudi Arabia Department of Computer Science and Information Taibah University Madinah Saudi Arabia Department of Electrical and Computer Engineering COMSATS University Islamabad45550Pakistan Department of Computer Science Faculty of Computers and InformaticsZagazig UniversityZagazig44511Egypt

Vehicular communication is the backbone of future Intelligent Transportation Systems(ITS).It offers a network-based solution for vehicle safety,cooperative awareness,and traffic management applications.For safety applications,Basic Safety Messages(BSM)containing mobility information is shared by the vehicles in their neighborhood to continuously monitor other nearby vehicles and prepare a local traffic map.BSMs are shared using mode 4 of Cellular V2X(C-V2X)communications in which resources are allocated in an ad hoc manner.However,the strict packet transmission requirements of BSM and hidden node problem causes packet collisions in a vehicular network,thus reducing the reliability of safety applications.Moreover,as vehicles choose the transmission resources in a distributed manner in mode 4 of CV2X,the packet collision problem is further aggravated.This paper presents a novel solution in the form of a Space Division Multiple Access(SDMA)protocol that intelligently schedules BSM transmissions using vehicle position data to reduce concurrent transmissions from hidden node interferers.The proposed protocol works by dividing road segments into clusters and subclusters.Several sub-frames are allocated to a cluster and these sub-frames are reused after a certain distance.Within a cluster,sub-channels are allocated to sub-clusters.We implement the proposed SDMA protocol and evaluate ITS performance in a highway vehicular network.Simulation results show that the proposed SDMA protocol outperforms standard Sensing-Based Semi Persistent Scheduling(SB-SPS)in terms of safety range and packet delay.

关键词： ITS vehicular network cellular V2X SDMA

李玉乳菇(新种):欧洲槭乳菇在亚洲的近缘种

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菌物学报 2023年第1期42卷 252-262页

作者：曹书琴王向华秦位强吴芳刘铁志图力古尔李双龙陈作红刘冬梅中国科学院昆明植物研究所、东亚植物多样性与生物地理学重点实验室、云南省真菌多样性与绿色发展重点实验室云南昆明650201 吉首大学张家界校区湖南张家界427000 湖南省食用菌研究所湖南长沙410013 赤峰学院化学与生命科学学院内蒙古赤峰024000 吉林农业大学菌物研究所吉林长春130118 恩施土家族苗族自治州林业科学研究院湖北恩施445000 湖南师范大学生命科学学院湖南长沙410081 中国环境科学研究院北京100012

槭乳菇Lactarius acerrimus,乳菇中仅有的具有双孢担子的物种,曾经在中国被多次报道。我们在研究了该种的馆藏凭证标本后并未证实它在中国的分布,而是在中国发现了它的近缘种李玉乳菇L.liyuanus(新种)。李玉乳菇的典型特征为:菌盖黄褐... 详细信息

槭乳菇Lactarius acerrimus,乳菇中仅有的具有双孢担子的物种,曾经在中国被多次报道。我们在研究了该种的馆藏凭证标本后并未证实它在中国的分布,而是在中国发现了它的近缘种李玉乳菇L.liyuanus(新种)。李玉乳菇的典型特征为:菌盖黄褐色具环纹、边缘具短毛,菌柄具窝斑,乳汁白色、不变色、具辣味,孢子具尖而高的条脊。它的担子为4孢,孢子较小具高而尖的条脊,这两点与槭乳菇明显不同。文中提供了该种的条形码ITS序列、其他多基因序列和详细的形态描述,同时给出了对国内馆藏槭乳菇标本的重新鉴定结果。

关键词：壳斗科 ITS 真菌区系物种识别

Soil microbial communities as potential regulators of N2O sources in highly acidic soils

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Soil Ecology Letters 2023年第4期5卷 81-87页

作者： Junhui Yin Huaihai Chen Pengpeng Duan Kun Zhu Naihui Li Yan Ma Yumeng Xu Jingheng Guo Rui Liu Qing Chen Beijing Key Laboratory of Farmyard Soil Pollution Prevention-control and Remediation College of Resources and Environmental SciencesChina Agricultural UniversityBeijing 100193China State Key Laboratory of Biocontrol School of EcologySun Yat-sen UniversityGuangzhou 510006China Key Laboratory of Agro–Ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China College of Land Science and Technology China Agricultural UniversityBeijing 100193China Department of Horticulture Northeast Agricultural UniversityHarbin 150030China

●Soil pH was a key driver of N2O emission and sources in acidic soils.●N2O emission was significantly positively associated with the ratio of ITS to 16S.●N2O was significantly correlated with bacterial and fungal community composition.●Fungi contributed to N2O in highly acidic tea plantations and vegetable fields.Acidic soil is a main source of global nitrous oxide(N2O)emissions.However,the mechanism behind the high N2O emissions from acidic soils remains a knowledge gap.The objective of this microcosm incubation study was to pin-point the microbial mechanisms involved in N2O production processes in acidic soils.For that purpose,the isotopic signatures and microbial community structure and composition of four soil samples were examined.The results showed that highly acid soils(pH=3.51)emitted 89 times more N2O than alkaline soils(pH=7.95)under the same nitrogen(N)inputs.Fungal denitrification caused high N2O emissions in acidic soils.ITS to 16S abundance ratio was positively correlated with cumulative N2O emissions from the tested soils.The highly acid soils(pH<4.5)showed greater fungal nirK gene abundance and lower abundance of AOA-amoA,AOB-amoA,nirK,nosZ I and nosZ II genes.The unclassified Aspergillaceae fungi(63.65%)dominated the highly acidic soils and was the most strongly correlated genus with N2O emissions.These findings highlight that soil microbial community structures,denitrifying fungi in particular,shaped by low pH(pH<4.5)lead to high N2O emissions from acidic soils.

关键词： nitrous oxide ITS 16S ratio fungal denitrification soil pH vegetable field tea plantation

Anatomical and Molecular Identification of Ornamental Plant Ficus L.Species

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Phyton-International Journal of Experimental Botany 2023年第5期92卷 1329-1347页

作者： Abtisam Binnoubah Rim Hamdy Osama G.Ragab Ahmed M.El-Taher Ahmed Abou El-Yazied Fatmah A.Safhi Hala A.Elzilal Ashwaq T.Althobaiti Salha M.ALshamrani Diaa Abd El Moneim Ahmed El-Banhawy Department of Botany Faculty of ScienceUniversity of SabrathaSabratha999116Libya Department of Botany Faculty of ScienceCairo UniversityGiza12111Egypt Department of Botany and Microbiology Faculty of Science(Boys Branch)Al-Azhar UniversityCairo11884Egypt Department of Agricultural Botany Faculty of AgricultureAl-Azhar UniversityCairo11884Egypt Department of Horticulture Faculty of AgricultureAin Shams UniversityCairo11566Egypt Department of Biology College of SciencePrincess Nourah bint Abdulrahman UniversityRiyadh11671Saudi Arabia Department of Science and Technology College of RanyahTaif UniversityTaif21944Saudi Arabia Department of Biology College of ScienceTaif UniversityTaif21944Saudi Arabia Department of Biology College of ScienceUniversity of JeddahJeddah21959Saudi Arabia Department of Plant Production(Genetic Branch) Faculty of Environmental Agricultural SciencesArish UniversityEl-Arish45511Egypt Department of Botany and Microbiology Faculty of ScienceSuez Canal UniversityIsmailia41522Egypt

This present study includes twelve species that represent the Ficus genus,namely;aspera,carica,tinctoria subsp.gibbosa,hirta,hispida,neriifolia,palmata,pumila,racemosa,septica,sur,and sycomorus,belonging to the Moraceae family.The species samples were collected from various locations in Egypt.The study focused on the anatomical and molecular characteristics of mature foliage leaves.Since the identification and classification of taxa are highly dependent on the anatomical features of leaves,the anatomical characteristics were recorded in the form of a comparison between the examined plants in the data matrix.This study aims to contribute to the identification of the studied species based on the anatomical details of the matured leaves.Anatomical characterization includes the variations in upper and lower epidermal layers that are covered by a thin or thick cuticle;the number of palisade and spongy layers;crystals;secretory elements;lithocysts;the midrib zone has parenchyma associated with mechanical tissue,vascular system,and investigation of trichomes;on the other hand,in the current study,the phylogenetic analysis was conducted by using the ITS and 5.8 S sequences.From the analysis of all the available data,it could be stated that there is an overall agreement with the anatomical character dendrogram.

关键词： ITS trichomes leaf anatomy Ficus moraceae

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桃仁及苦杏仁DNA检测试剂盒的研制与评价

中国药学杂志 2022年第6期57卷 478-485页

作者：宋顺佳姜菲菲关奕泽赵远贾慧建邵学超艾金霞孙丽媛北华大学医学技术学院吉林吉林132013

目的本研究旨在研制一种快速检测试剂盒,鉴定桃仁及苦杏仁DNA成分,并对其进行性能评价。方法建立DNA提取方法,确保有效提取桃仁及苦杏仁基因组DNA,紫外分光光度法测其浓度及纯度。美国国立生物技术信息中心(NCBI)查询并比对桃仁及苦杏仁... 详细信息

目的本研究旨在研制一种快速检测试剂盒,鉴定桃仁及苦杏仁DNA成分,并对其进行性能评价。方法建立DNA提取方法,确保有效提取桃仁及苦杏仁基因组DNA,紫外分光光度法测其浓度及纯度。美国国立生物技术信息中心(NCBI)查询并比对桃仁及苦杏仁的ITS序列,针对特异性位点,NCBI Primer-Blast设计种属特异性引物。建立并优化聚合酶链式反应(PCR)检测体系,DH5α感受态细胞克隆特异性片段,测序验证体系准确性,制备阳性对照。组装DNA检测试剂盒,对其特异性、稳定性及重复性进行评价,并对市售桃仁和苦杏仁样品进行检测。结果所建立DNA提取方法,提取效率高,DNA质量浓度高于100 ng·μL^(-1),A_(260/280)处于1.80~2.10之间。该试剂盒可准确鉴定桃仁及苦杏仁DNA成分,检测灵敏度高,特异性强,最低检测限为1 ng·μL^(-1)。反复冻融20次后仍可有效检测,可于-20℃保存1年。20个市售样品中检出1个桃仁伪品及2个苦杏仁伪品,其余均为正品。结论桃仁及苦杏仁DNA检测试剂盒特异性强、灵敏性高、稳定性好,具有良好重复性,可快速鉴定桃仁及苦杏仁,为桃仁中药材市场质量安全管理提供有力支持。

关键词：桃仁苦杏仁 ITS 试剂盒聚合酶链式反应

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钩藤属植物分子鉴定的DNA条形码筛选

中草药 2022年第6期53卷 1828-1837页

作者：蔡一鸣代江鹏郑雨欣任英毅陈浩明冯婷婷高晓霞朱爽广东药科大学生命科学与生物制药学院广东省生物技术候选药物研究重点实验室广东广州510006 广东药科大学药学院广东广州510006

目的应用分子生物学鉴定技术,筛选出应用于鉴定钩藤属物种的最佳DNA条形码,建立快速、准确、便捷的钩藤属植物鉴定方法。方法从广东、广西等地收集了8个钩藤属物种的叶片、茎枝作为材料,共计44份样品。提取样品总DNA,分别对条形码ITS、m... 详细信息

目的应用分子生物学鉴定技术,筛选出应用于鉴定钩藤属物种的最佳DNA条形码,建立快速、准确、便捷的钩藤属植物鉴定方法。方法从广东、广西等地收集了8个钩藤属物种的叶片、茎枝作为材料,共计44份样品。提取样品总DNA,分别对条形码ITS、matK、psbA-trnH、rbcL、trnL-trnF序列进行扩增、测序、拼接、校对;利用MEGA 7.0分析比对序列特征;基于Taxon DNA计算种内、种间的遗传距离以分析barcoding gap及Best Match、Best Close Match评估DNA条形码的鉴别能力;使用MEGA7.0、Phylosuite等软件构建单条形码及组合条形码的最大似然法(maximum likelihood,ML)、最大简约法(maximum parsimony,MP)、贝叶斯推断法(bayesian inference,BI)系统发育树。结果条形码ITS、matK、psbA-trnH、rbcL、trnL-trn F序列均扩增成功且具有较高的测序成功率,其中psb A-trn H具有最多的变异位点,ITS次之,rbc L最少;Best Match、Best Close Match与Barcoding gap分析结果显示:5个单一条形码中,ITS鉴定钩藤属物种效果突出且具有明显的Barcoding gap,而组合条形码ITS+rbcL序列表现更为优异。基于所有单一条形码构建的系统发育树,ITS序列的物种鉴别成功率最高,能够准确鉴定8个钩藤属物种;基于组合条形码构建的系统发育树,ITS+rbcL序列具有最高的平均节点支持率,且该序列集包含的11个钩藤属物种均能单独聚为一支。结论应用ITS+rbc L的序列组合能够实现钩藤属不同物种的准确鉴定。

关键词：钩藤属 DNA条形码物种鉴定 ITS rbcL TaxonDNA 系统发育分析

冬季赤水河流域刚毛藻多样性调查及系统发育分析

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水生生物学报 2022年第9期46卷 1402-1411页

作者：赵志娟杨小霞钟俏君陈冠海彭相英刘本文朱欢刘国祥南宁师范大学北部湾环境演变与资源利用教育部重点实验室南宁530001 南宁师范大学广西地表过程与智能模拟重点实验室南宁530001 中国科学院水生生物研究所中国科学院藻类生物学重点实验室武汉430072

研究于2019年冬季对赤水河流域开展刚毛藻多样性调查,共设计采样点38个,覆盖赤水河上、中、下游。调查发现:21个采样位点分布有刚毛藻目藻类,其中19个位点有刚毛藻分布。基于核糖体小亚基(SSU rDNA)、核糖体大亚基(LSU rDNA)和内转录间... 详细信息

研究于2019年冬季对赤水河流域开展刚毛藻多样性调查,共设计采样点38个,覆盖赤水河上、中、下游。调查发现:21个采样位点分布有刚毛藻目藻类,其中19个位点有刚毛藻分布。基于核糖体小亚基(SSU rDNA)、核糖体大亚基(LSU rDNA)和内转录间隔区(ITS)对采集样品进行系统发育分析,结果显示:(1)赤水河流域刚毛藻多样性较高,且该流域上、中、下游均有刚毛藻分布;(2)目前淡水刚毛藻类群包含至少10个支系,赤水河流域采集到的刚毛藻覆盖其中6个支系(分别是clade 1、clade 2、clade 4、clade 7、clade 9和clade 10);(3)相比基于SSU+LSU双分子标记构建的系统进化树,基于SSU+ITS+LSU三分子标记构建的进化树各支系支持度更高,可以较有效地将淡水刚毛藻不同支系区分开来。研究较好地展示了冬季赤水河流域刚毛藻的广泛分布及其分子多样性,丰富了中国淡水丝状绿藻的分类研究,也为赤水河段的水生态环境保护提供了基础数据支持。

关键词：赤水河流域刚毛藻目多样性 SSU rDNA LSU rDNA ITS 系统发育分析