检索结果-南通市图书馆

Toxoplasma ROP16Ⅰ/Ⅲ ameliorated inflammatory bowel diseases via inducing M2 phenotype of macrophages

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2019年第45期25卷 6634-6652页

作者： Yong-Wei Xu Rui-Xin Xing Wen-Hui Zhang Lu Li Yi Wu Jing Hu Cong Wang Qing-Li Luo Ji-Long Shen Xi Chen Department of Gastroenterology the First Affiliated Hospital of Anhui Medical UniversityHefei 230022Anhui ProvinceChina Department of Pathogen Biology Provincial Laboratory of Pathogen Biology and Zoonoses AnhuiAnhui Medical UniversityHefei 230032Anhui ProvinceChina

BACKGROUND Inflammatory bowel disease(IBD)is characterized by chronic and non-specific inflammation of the intestinal mucosa and mainly includes ulcerative colitis and Crohn's *** To explore the beneficial effect of ToxoROP16I/III-induced M2 phynotype macrophages in homeostasis of IBDs through downregulation of M1 inflammatory *** RAW264.7 macrophages stimulated by lipopolysaccharide(LPS)(M1 cells)were co-cultured with Caco-2 cells as an inflammatory model of IBD in *** expression of ToxoROP16I/III was observed in RAW264.7 macrophages that were transfected with pEGFP-rop16I/*** phenotypes of M2 and M1 macrophage cells were assessed by quantitative real-time reverse transcriptase polymerase chain reaction and the expression of tumor necrosis factor(TNF)-α,interleukin(IL)-1β,IL-6,transforming growth factor(TGF)-β1,IL-10,inducible nitric oxide synthase(iNOS),and arginase-1(Arg-1)was *** expression of iNOS,Arg-1,signal transducer and activator of transcription 3(Stat3),p-Stat3,Stat6,p-Stat6,programmed death ligand-2(PD-L2),caspase-3,-8,and-9 was analyzed by Western blotting,and Griess assays were performed to detect nitric oxide(NO).TNF-α,IL-1β,IL-6,TGF-β1,and IL-10 expression in the supernatants was detected by enzyme-linked immunosorbent assay,and Caco-2 cell apoptosis was co-culture *** M1 cells exhibited significantly increased production of iNOS,NO,TNF-α,IL-1β,and IL-6,while ToxoROP16I/III induced macrophage bias to M2 cells in vitro,showing increased expression of Arg-1,IL-10 and TGF-β1 and elevated production of p-Stat3 and *** mixed M1 and M2 cell culture induced by ToxoROP16I/III exhibited decreased production of NO and iNOS and upregulated expression of Arg-1 and ***,Caco-2 cells became apoptotic,and apoptosis-associated proteins such as caspase-3,-8 and-9 were dampened during co-culture of M1 and M2 *** cytometry analysis showed that co-culture of M1 cells with Caco-2 cells facilitat

关键词： Toxoplasma ROP16I/III Caco-2 Inflammatory bowel disease Immunity Classically activated macrophages Alternatively activated macrophages

Use of siRNA molecular beacons to detect and attenuate mycobacterial infection in macrophages

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Experimental Medicine 2015年第3期5卷 164-181页

作者： Remo George Renata Cavalcante Celso Carvalho Jr Elyana Marques Jonathan B Waugh M Tino Unlap Center for Teaching and Learning and the Department of Clinical and Diagnostic Sciences University of Alabama at Birmingham

Tuberculosis is one of the leading infectious diseases plaguing mankind and is mediated by the facultative pathogen, Mycobacterium tuberculosis(MTB). Once the pathogen enters the body, it subverts the host immune defenses and thrives for extended periods of time within the host macrophages in the lung granulomas, a condition called latent tuberculosis(LTB). Persons with LTB are prone to reactivation of the disease when the body's immunity is compromised. Currently there are no reliable and effective diagnosis and treatment options for LTB, which necessitates new research in this area. The mycobacterial proteins and genes mediating the adaptive responses inside the macrophage is largely yet to be determined. Recently, it has been shown that the mce operon genes are critical for host cell invasion by the mycobacterium and for establishing a persistent infection in both in vitro and in mouse models of tuberculosis. The Yrb E and Mce proteins which are encoded by the MTB mce operons display high degrees of homology to the permeases and the surface binding protein of the ABC transports, respectively. Similarities in structure and cell surface location impute a role in cell invasion at cholesterol rich regions and immunomodulation. The mce4 operon is also thought to encode a cholesterol transport system that enables the mycobacterium to derive both energy and carbon from the host membrane lipids and possibly generating virulence mediating metabolites, thus enabling the bacteria in its long term survival within the granuloma. Various deletion mutation studies involving individual or whole mce operon genes have shown to be conferring varying degrees of attenuation of infectivity or at times hypervirulence to the host MTB, with the deletion of mce4 A operon gene conferring the greatest degree of attenuation of virulence. Antisense technology using synthetic si RNAs has been used in knocking down genes in bacteria and over the years this has evolvedinto a powerful tool for el

关键词： Mammalian cell entry Molecular beacons siRNA Mycobacterium tuberculosis macrophages

Tumor-associated macrophages, exosomes and tumor metastasis: a mini-review

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

TMR Cancer 2020年第3期3卷 119-126页

作者： Xiao-Dong Wang First Affiliated Hospital of Zhejiang University School of Medicine Hangzhou 310003China

Tumor invasion and metastasis is the primary cause of death in patients with malignant ***-associated macrophages are macrophages that infiltrate the tumor tissue,and are the most frequent immune cells in the tumor *** macrophages could secrete a variety of cytokines and can recognize,identify and clear the tumor *** the tumor develops,it plays a vital role in the growth,invasion and metastasis of the ***,the underline mechanisms of how the tumor-associated macrophages promoting tumor metastasis is still not fully *** studies demonstrated that macrophage-related exosomes might play an essential role in tumor *** may provide potential targets for the prevention and treatment of macrophage-related tumor metastasis.

关键词： Exosomes Cancer metastasis macrophages Treatment targets

Impact Factor:1.90 Total flavonoids from Saussurea involucrata attenuate inflammation in lipopolysaccharide-stimulated RAW264.7 macrophages via modulating p65,c-Jun,and IRF3 signaling pathways

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2021年第6期11卷 273-284页

作者： Li-Shan Yan Li Wang Brian Chi-Yan Cheng Yu Ding Jing Kong Qing Gao Wang Xiu-Qiong Fu Shuo-Feng Zhang Gan Luo Yi Zhang School of Chinese Materia Medica Beijing University of Chinese MedicineBeijingChina College of Professional and Continuing Education Hong Kong Polytechnic UniversityHong KongChina The First Affiliated Hospital Guangxi University of Chinese MedicineGuangxiChina Centre for Cancer and Inflammation Research School of Chinese MedicineHong Kong Baptist UniversityHong KongChina

Objective:To investigate the anti-inflammatory effects of the total flavonoids from Saussurea involucrata on lipopolysaccharides(LPS)-stimulated murine RAW264.7 macrophages and explore its underlying mechanism of ***:Total flavonoids from Saussurea involucrata were extracted using chromatographic column *** viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide *** production of nitric oxide was detected by Griess assay and the release of cytokines(IL-10 and TNF-α)and chemokines(MCP-1,MIP-1α,and CCL5/RANTES)was determined by ELISA to evaluate the anti-inflammatory activity of total flavonoids from Saussurea ***,nuclear translocation of p65,c-Jun,and IRF3 was detected by immunofluorescence microscopy and Western blotting analysis was performed to determine the expression of related ***:Total flavonoids extracted from Saussurea involucrata were 751.5 mg/g and the content of rutin was 506.5 mg/*** production of inflammatory mediators including nitric oxide,cytokines,and chemokines was effectively inhibited by total flavonoids from Saussurea ***,total flavonoids also suppressed the nuclear translocation of p65,c-Jun,and IRF3 in LPS-stimulated RAW264.7 *** LPS-induced expression of iNOS and COX-2 was remarkably reduced by treatment with total flavonoids from Saussurea ***,total flavonoids decreased the expression levels of p-IKKα/β,p-TBK1,p-p38,p-ERK,p-JNK,p-p65,p-c-Jun,and p-IRF3 in LPS-exposed RAW264.7 ***:Total flavonoids from Saussurea involucrata potentially inhibit the secretion of pro-inflammatory mediators,which may be related to inhibition of p65,c-Jun,and IRF3 signaling pathways in LPS-stimulated RAW264.7 cells.

关键词： Saussurea involucrata Cytokines Proinflammatory macrophages Flavonoids

The impact of telmisartan on angiotensin converting enzyme 2 mRNA expression in monocyte-derived macrophages of diabetic hypertensive patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

China Medical Abstracts(Internal Medicine) 2013年第1期30卷 21-21页

作者：李永勤 Dept Cardiol 2nd HospXi'an Jiaotong Univ

Objective To investigate the effects of telmisartan on the expression of angiotensin converting enzyme 2(ACE2) mRNA in monocyte-derived macrophages of hypertensive patients accompanied with diabetes. Methods 62 essent... 详细信息

关键词： hypertensive converting macrophages monocyte accompanied hemoglobin metabolism diseased decreasing creased

Role of tumor associated macrophages in regulating pancreatic cancer progression

在线全文

学校读者我要写书评

暂无评论

World Journal of Immunology 2016年第1期6卷 9-18页

作者： Raul Caso George Miller NYU School of Medicine NYU Langone Medical Center Departments of Surgery and Cell Biology NYU Langone Medical Center

Pancreatic cancer has an overall 5-year survival rate of less than 5%.Unfortunately,patient survival has not substantially improved in the last couple of decades despite advances in treatment modalities that have been successful in other cancer *** poor response of pancreatic cancer to therapy is a majo obstacle faced by *** attention is bein paid to how tumor cells and non-tumor cells influenc each other in the pancreatic tumor microenvironment Tumor-associated macrophages(TAMs)are a highligh in this field because of their vast presence in th tumor *** promote angiogenesis metastasis,and suppress the anti-tumor immun *** we review the current understandin of the role of TAMs in regulating the progression o pancreatic cancer.

关键词： Pancreatic cancer Tumor-associated macrophages Tumor microenvironment macrophages

Role of macrophages in the progression of acute pancreatitis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Pharmacology and Therapeutics 2010年第5期1卷 107-111页

作者： Sabrina Gea-Sorlí Daniel Closa Department of Experimental Pathology IIBB-CSIC-IDIBAPS-CIBEREHDBarcelona 08036Spain

In addition to pancreatic cells,other inflammatory cell populations contribute to the generation of inflammatory mediators during acute *** particular,macrophages could be activated by mediators released during pancreatitis by a damaged *** has been reported that peritoneal macrophages,alveolar macrophages and Kupffer cells become activated in different stages of severe acute ***,macrophages display remarkable plasticity and can change their physiology in response to environmental *** on their microenvironmental stimulation,macrophages could follow different activation pathways resulting in marked phenotypic *** ability has made these cells interesting therapeutical targets and several approaches have been assayed to modulate the progression of inflammatory response secondary to acute ***,despite the recent advances in the modulation of macrophage function in vivo,the therapeutical applications of these strategies require a better understanding of the regulation of gene expression in these cells.

关键词： Pancreatitis macrophages Inflammation Systemic inflammatory response syndrome

Immunotherapy of melanoma metastasis by adoptive transfer of M-CSF or/and IFN-γ gene modified macrophages pulsed with tumor extracts

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国实验血液学杂志 1997年第3期5卷 312-312页

作者： Hong Lei, Yizhi Yu, Xuetao CaoDepartment of Immunology, The Second Military MedicalUniversity, Shanghai 200433

Induction of tumor-specific cellular immune responseis very important in the cancer therapy. In this study,we used tumor antigen obtained by thaw of melanomacells to pulse M-CSF or/and IFN-γ gene-modificdmacrophages before in viro infusion. Tumor membraneantigens could be phagocytosed by macrophages inculture. Antigen processing and mcxlulation of thepresentation can be achieved before macrophageinjection. The tumor antigens will be processedintracellularly by macrophages and thereafter

关键词： melanoma macrophages Immunotherapy extracts metastasis infusion thereafter presentation Antigen Induction

Effect of high glucose on the expression of CD36 and lipid accumulation in THP-1 macrophages

在线全文

学校读者我要写书评

暂无评论

China Medical Abstracts(Internal Medicine) 2014年第3期31卷 163-163页

作者：谭玉林 Instit Cardiovasc Res & Key Lab South China Univ

Objective To investigate the effect of high glucose on regulating the expression of CD36 and lipid accumulation in THP-1 *** THP-1 macrophages were incubated with different concentrations of D-glucose(5.6,11,20,30 and 35 mmol/L),50 mg/L oxidized low density lipoprotein(ox-LDL),50 mg/L oxLDL+20 mmol/L D-glucose for 24 *** cholesterol content in THP-1 macrophages was determined by high performance liquid chromatography,the lipid accumulation was detected by oil red O ***36 mRNA

关键词： macrophages lipid oxLDL regulating cholesterol oxidized incubated stain regulate treating