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Induction of Epstein-Barr Virus lytic replication by Recombinant Adenoviruses Expressing the Zebra Gene with EBV Specific Promoters

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Acta Biochimica et Biophysica Sinica 2005年第4期37卷 215-220页

作者： Lu CHEN Juan YIN Yi CHEN Jiang ZHONG Department of Microbiology and Microbial Engineering,School of Life Sciences,Fudan University,Shanghai 200433,China Department of Microbiology and Microbial Engineering School of Life Sciences Fudan University Shanghai 200433 China

The latent Epstein-Barr virus (EBV) is found in the cells of many *** example,EBVis detectable in almost all cases,and in almost all tumor cells,of non-keratinizing nasopharyngeal *** the latent virus,which will result in its lytic replication and the death of tumor cells,is a potentialapproach for the treatment of EBV-associated *** this study,three recombinant adenoviruses wereconstructed to express the Zebra gene,an EBV gene responsible for switching from the latent state to ***-specific promoters were used in order to limit Zebra expression in EBV-positive cells,andreduce the potential side *** EBV promoters used were Cp,Zp and a dual promoter combining *** Zebra protein was detected in HEK293 cells as well as the EBV-positive D98-HR1cells infected with recombinant *** EBV lyric replication early antigen,EA-D,was also detected ininfected D98-HR1,implying the initiation of lytic *** the cell viability assay,Zebra-expressingadenoviruses had little effect on EBV-negative HeLa cells,while significantly reducing the cell viability andproliferation of D98-HR1 *** results indicate that EBV virus promoters can be used in adenovirusvectors to express the Zebra gene and induce EBV lytic replication in D98-HR1 cells.

关键词： Epstein-Barr virus recombinant adenovirus lytic replication cancer

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CCAAT/enhancer binding proteins play a role in oriLyt-dependent genome replication during MHV-68 de novo infection

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Protein & Cell 2011年第6期2卷 463-469页

作者： Jing Qi Danyang Gong Hongyu Deng CAS Key Laboratory of Infection and Immunity Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Graduate School of the Chinese Academy of Sciences Beijing 100080China

Murine gammaherpesvirus 68(MHV-68),a member of the gammaherpesvirus family,replicates robustly in permissive cell lines and is able to infect laboratory ***-68 has emerged as a model for studying the basic aspects of viral replication and host–virus interactions of its human *** genome replication is mediated through a cis-element in the viral genome called the origin of lytic replication(oriLyt).A family of transcription factors,CCAAT/enhancer binding proteins(C/EBPs),assists in oriLyt-mediated DNA replication during gammaherpesvirus *** this study,we examined the role of C/EBPs in gammaherpesvirus DNA replication during de novo infection,using MHV-68 as a *** found that C/EBP α and β bind to the CCAAT boxes in the MHV-68 oriLyt core region both in vitro and in vivo,as demonstrated by electrophoretic mobility shift assay and chromatin immunoprecipitation assay.A dominant negative form of C/EBPs significantly impaired the lytic replication efficiency of MHV-68 on both the plasmid and genome levels in a replication assay,indicating that functional C/EBPs are required for maximal MHV-68 genome DNA ***,our data demonstrate that C/EBPs interact with the oriLyt core region and play an important role in MHV-68 lytic DNA replication during de novo infection.

关键词： C/EBPs murine gammaherpesvirus 68 oriLyt lytic replication

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