CCAAT/enhancer binding proteins play a role in oriLyt-dependent genome replication during MHV-68 de novo infection

作     者：Jing Qi Danyang Gong Hongyu Deng 

作者机构：CAS Key Laboratory of Infection and ImmunityInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Graduate School of the Chinese Academy of SciencesBeijing 100080China 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2011年第2卷第6期

页      面：463-469页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(Grant No.30930007) 

主　　题：C/EBPs murine gammaherpesvirus 68 oriLyt lytic replication 

摘      要：Murine gammaherpesvirus 68(MHV-68),a member of the gammaherpesvirus family,replicates robustly in permissive cell lines and is able to infect laboratory ***-68 has emerged as a model for studying the basic aspects of viral replication and host–virus interactions of its human *** genome replication is mediated through a cis-element in the viral genome called the origin of lytic replication(oriLyt).A family of transcription factors,CCAAT/enhancer binding proteins(C/EBPs),assists in oriLyt-mediated DNA replication during gammaherpesvirus *** this study,we examined the role of C/EBPs in gammaherpesvirus DNA replication during de novo infection,using MHV-68 as a *** found that C/EBP α and β bind to the CCAAT boxes in the MHV-68 oriLyt core region both in vitro and in vivo,as demonstrated by electrophoretic mobility shift assay and chromatin immunoprecipitation assay.A dominant negative form of C/EBPs significantly impaired the lytic replication efficiency of MHV-68 on both the plasmid and genome levels in a replication assay,indicating that functional C/EBPs are required for maximal MHV-68 genome DNA ***,our data demonstrate that C/EBPs interact with the oriLyt core region and play an important role in MHV-68 lytic DNA replication during de novo infection.