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lutein delays photoreceptor degeneration in a mouse model of retinitis pigmentosa

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Neural Regeneration Research 2022年第7期17卷 1596-1603页

作者： Hui-Jun Zhang Xiao-Bin Liu Xiong-Min Chen Qi-Hang Kong Yu-Sang Liu Kwok-Fai So Jian-Su Chen Ying Xu Xue-Song Mi Shi-Bo Tang Department of Ophthalmology the First Affiliated Hospital of Jinan UniversityGuangzhouGuangdong ProvinceChina Department of Ophthalmology Guangzhou Panyu Central HospitalGuangzhouGuangdong ProvinceChina Guangdong-Hong Kong-Macao Institute of CNS Regeneration Jinan UniversityGuangzhouGuangdong ProvinceChina Laboratory of Retinal Cell Biology Aier Eye InstituteChangshaHunan ProvinceChina Aier Academician Station ChangshaHunan ProvinceChina Key Laboratory of CNS Regeneration(Jinan University) Ministry of EducationGuangzhouGuangdong ProvinceChina CoInnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina

Retinitis pigmentosa is a retinal disease characterized by photoreceptor *** is currently no effective treatment for retinitis *** a mixture of lutein and other antioxidant agents has shown promising effects in protecting the retina from degeneration,the role of lutein alone remains *** this study,we administered intragastric lutein to Pde6brd10 model mice,which display degeneration of retinal photoreceptors,on postnatal days 17(P17)to P25,when rod apoptosis reaches *** at the optimal protective dose of 200 mg/kg promoted the survival of photoreceptors compared with vehicle *** increased rhodopsin expression in rod cells and opsin expression in cone cells,in line with an increased survival rate of ***,lutein improved visual behavior,visual acuity,and retinal electroretinogram responses in Pde6brd10 ***,lutein reduced the expression of glial fibrillary acidic protein in Müller glial *** results of this study confirm the ability of lutein to postpone photoreceptor degeneration by reducing reactive gliosis of Müller cells in the retina and exerting anti-inflammatory *** study was approved by the Laboratory Animal Ethics Committee of Jinan University(approval ***-20181217-02)on December 17,2018.

关键词： anti-inflammation glial fibrillary acidic protein lutein microglia Pde6brd10(rd10)mouse photoreceptor reactive gliosis retinal degeneration retinal disease retinitis pigmentosa

lutein and lutein esters in marigold flowers by high performance chromatography

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Journal of Central South University of Technology 2005年第3期12卷 306-308页

作者：蒋新宇陈龙胜周春山 School of Chemistry and Chemical Engineering Central South UniversityChangsha 410083China

lutein and lutein esters in marigold flowers was quantitatively determined by high performance chromatography （HPLC） with ODS-C 18 column.A mixture of CH3CNCD*2CH3OHCD*2CH3COOCH2CH3 with volume ratio of 55∶1∶44 was used as mobile phase at a flow rate of 1.0 mL/min and detection was carried out at 460nm. The column temperature was about 20℃. The contents of lutein and lutein esters were determined by analytical curve of lutein since lutein and lutein esters have the same spectral characteristics. Determination results of hexane extracts and saponified samples of lutein show that the saponification transforms the esters into free lutein. The increase of the content of dipalmitate and palmitate stearate reveals that the reaction includes transesterifications.

关键词： HPLC lutein lutein ester marigold flower

Sources,dynamics in vivo,and application of astaxanthin and lutein in laying hens:A review

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Animal Nutrition 2023年第2期13卷 324-333页

作者： Hanyi Shi Xuejuan Deng Xiaoyu Ji Ning Liu Huiyi Cai Department of Animal Science Henan University of Science and TechnologyLuoyang471023HenanChina Department of Poultry Science University of GeorgiaAthens30602GAUSA National Engineering Research Center of Biological Feed Beijing100081China

Astaxanthin(Ax)and lutein are important fat-soluble pigments and essential nutrients for human and animal *** pluvialis microalga and Phaffia rhodozyma yeast are ideal species for commercial Ax *** flowers are a main source of commercial *** of dietary Ax and lutein in the gastrointestinal tract are similar to lipids,but their activities are tremendously challenged by many physiological and dietary factors;few data are available about these in *** Ax and lutein have insignificant effects on egg production and egg physical properties,but have pronounced effects on yolk color,nutrition,and *** two pigments can also enhance antioxidative capacity and immune function of laying hens.A few studies have shown that Ax and lutein can improve fertilization and hatchability of laying *** the pigmentation and health benefits of Ax and lutein from hen feed to human food,the commercial availability,chicken yolk improvement,and immune function of Ax and lutein are the focuses of this *** potential roles of carotenoids in the cytokine storm and gut microbiota are also briefly *** bioavailability,metabolism,and deposition of Ax and lutein in laying hens are suggested for future research.

关键词： Application Astaxanthin Egg yolk Food chain Laying hen lutein

Chromosome-scale genome assembly of marigold (Tagetes erecta L.):An ornamental plant and feedstock for industrial lutein production

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Horticultural Plant Journal 2023年第6期9卷 1119-1130页

作者： Haibo Xin Fangfang Ji Jie Wu Shiya Zhang Caijie Yi Shiwei Zhao Richen Cong Liangjun Zhao Hua Zhang Zhao Zhang Beijing Key Laboratory of Greening Plants Breeding Beijing Academy of Forestry and Landscape ArchitectureBeijing 100102China Beijing Key Laboratory of Development and Quality Control of Ornamental Crops Department of Ornamental HorticultureChina Agricultural UniversityBeijing 100193China

Marigold(Tagetes erecta L., African marigold) is a widely grown ornamental plant and a main source of the carotenoid lutein for the industrial production of pharmaceuticals, food coloring, and feed additives. To gain a deeper understanding of the genetic mechanism of lutein in marigold, a chromosome-scale assembly of the marigold(T. erecta V-01) genome was completed based on Illumina, PacBio, and Hi-C reads. The707.21-Mb assembled genome consisted of 35 834 annotated protein-coding genes, with 97.7% genomic integrity. We anchored 87.8% of the contigs(covering 621.20 Mb) to 12 pseudochromosomes, bringing the scaffold N50 length to 54.15 Mb. Phylogenetic analysis showed that marigold was closely related to the Asteraceae species bitter vine(Mikania micrantha) and sunflower(Helianthus annuus), all three of which originated in the Americas. Marigold diverged from the sunflower clade 23.57 million years ago(MYA) and from M. micrantha 19.59 *** has undergone three whole-genome duplication events, as well as a recent whole-genome duplication event(WGD-2) common to *** and M. micrantha. Marigold gene families were significantly less expanded than those of M. micrantha or H. annuus, and the marigold genome contained significantly fewer interspersed repeats, which might account for its smaller genome. In addition, a range of candidate genes involved in the lutein biosynthetic pathway were identified. The high-quality reference genome obtained in this study provided a valuable genomic resource for studying the evolution of the Asteraceae family and for improving marigold breeding strategies.

关键词： T.erecta L. Genome Gene family Carotenoid lutein

Effect of sodium alginate-based hydrogel loaded with lutein on gut microbiota and inflammatory response in DSS-induced colitis mice

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Food Science and Human Wellness 2023年第6期12卷 2428-2439页

作者： Pengxiang Xu Shuwei Luo Jiangfeng Song Zhuqing Dai Dajing Li Cai’e Wu Institute of Agro-product Processing Jiangsu Academy of Agricultural SciencesNanjing 210014China College of Light Industry and Food Engineering Nanjing Forestry UniversityNanjing 210037China

In order to effectively deliver lutein to the inflamed colon and better exert its pharmacological activity,this paper constructed a sodium alginate hydrogel-based delivery system loaded with lutein nanoparticles,evaluated the regulation on the expression and secretion of related inflammatory factors in mice with colitis,and its impact on intestinal microbial *** results showed that comparing lutein crystal and its nanoparticle,lutein hydrogel alleviated dextran sodium sulfate(DSS)-induced colitis in mice more effectively by adjusting fecal heme content,colon tissue damage,and inflammatory factor ***,lutein hydrogel increased the expression of intestinal tight junction proteins zonula occluden-1(ZO-1),claudin-1 and occludin to maintain the integrity of the intestinal-barrier,inhibited the nuclear factor-κB(NF-κB)pathway and reduced expression and secretion of inflammatory factors including tumour necrosis factor-α(TNF-α),inducible nitric oxide synthase(iNOS),NOD-like receptors 3(NLRP3)and interleukin(IL)-1β.In addition,the intestinal microbial environment of mice with colitis was improved by down-regulating the relative abundance of Desulfovibrionaceae and up-regulating the relative abundance of Erysipelotrichaceae and *** a slow-release carrier to load lutein nanoparticles,sodium alginate-based hydrogel has potential application prospect.

关键词： lutein Hydrogel Colitis Intestinal microbiota Inflammation regulation

Metabolomic and transcriptomic analysis reveals the molecular mechanism by which blue light promotes lutein synthesis in strawberry

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Journal of Integrative Agriculture 2023年第6期22卷 1695-1703页

作者： CHEN Xiao-dong CAI Wei-jian XIA Jin YUAN Hua-zhao WANG Qing-lian PANG Fu-hua ZHAO Mi-zhen Institute of Pomology Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementNanjing 210000P.R.China

Carotenoids are an important component of the human diet, and fruit is a primary source of carotenoids. The synthesis and regulation of carotenoids in fruit are important contributors to the formation of fruit quality. In China, strawberry is one of the main seasonal fruits grown in the winter. Previous studies have shown that light has a significant effect on the metabolism of anthocyanins, sugars, and polyphenols in strawberry. However, the understanding of the role of light in regulating the metabolism of carotenoids in strawberry remains limited. This study investigated the effects of blue, red, yellow-green, and white light on carotenoid metabolism in *** light treatment promoted the synthesis of multiple carotenoids, including lutein, compared with the other three treatment groups. The RNA sequencing data revealed that blue light treatment promoted the expression of lycopene ε-cyclase(LCYE), and the transient overexpression of LCYE in strawberry fruit promoted lutein accumulation in strawberry. Overall, the results suggest that blue light can promote the synthesis of lutein in strawberry by inducing the expression of LCYE.

关键词： carotenoid LED light strawberry lutein

Modulation of mitochondrial respiration underpins neuronal differentiation enhanced by lutein

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Neural Regeneration Research 2019年第1期14卷 87-99页

作者： Kui Xie Sherry Ngo Jing Rong Allan Sheppard Liggins Institute University of Auckland

lutein is a dietary carotenoid of particular nutritional interest as it is preferentially taken up by neural tissues. Often linked with beneficial effects on vision, a broader role for lutein in neuronal differentiation has emerged recently, although the underlying mechanisms for these effects are not yet dear. The purpose of this study was to investigate the effect of lutein on neuronal differentiation and explore the associated underpinning mechanisms. We found that lutein treatment enhanced the differentiation of SH-SYSY cells, specifically increasing neuronal arborization and expression of the neuronal process filament protein microtubule-associated protein 2. This effect was mediated by the intracellular phosphoinositide-3-kinase （PI3K） signaling pathway. While PI3K activity is a known trigger of neuronal differentiation, more recently it has also been shown to modulate the metabolic state of cells. Our analysis of bioenergetics found that lutein treatment increased glucose consumption, rates of glycolysis and enhanced respiratory activity of mitochondrial complexes. Concomitantly, the generation of reactive oxygen species was increased （con- sistent with previous reports that reactive oxygen species promote neuronal differentiation）, as well as the production of the key metabolic intermediate acetyl-CoA, an essential determinant of epigenetic status in the cell. We suggest that lutein-stimulated neuronal differentiation is mediated by PI3K-dependent modulation of mitochondrial respiration and signaling, and that the consequential metabolic shifts initiate epigenetically dependent transcriptomic reprogramming in support of this morphogenesis. These obser- vations support the potential importance of micronutrients supplementation to neurogenesis, both during normal development and in regenerative repair.

关键词： lutein micronutrient neuronal differentiation metabolism PI3K-AKT pathway glycolysis mitochondria gene expression

Vitreoscilla hemoglobin gene(vgb)improves lutein production in Chlorella vulgaris

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Chinese Journal of Oceanology and Limnology 2014年第2期32卷 390-396页

作者：马瑞娟林祥志 Engineering Research Center of Marine Biological Resource Comprehensive Utilization Third Institute of OceanographyState Oceanic Administration

Vitreoscilla hemoglobin is an oxygen-binding protein that promotes oxygen delivery and reduces oxygen consumption under low oxygen conditions to increase the effi ciency of cell respiration and *** this study,we introduced a Vitreoscilla hemoglobin gene(vgb)into Chlorella vulgaris by Agrobacterium tumefaciens-mediated transformation(ATMT).PCR analysis confi rmed that the vgb gene was successfully integrated into the Chlorella vulgaris *** of biomass obtained in shake fl asks revealed transformant biomass concentrations as high as 3.28 g/L,which was 38.81% higher than that of the wild-type *** content of transformants also increased *** experiments recovered a maximum lutein yield of 2.91 mg/L from the transformants,which was 36.77% higher than that of the wild-type *** above results suggest that integrated expression of the vgb gene may improve cell growth and lutein yield in Chlorella vulgaris,with applications to lutein production from Chlorella during fermentation.

关键词： Chlorella vulgaris Vitreoscilla hemoglobin gene (vgb) genetic engineering lutein

Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells

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International Journal of Ophthalmology(English edition) 2023年第1期16卷 40-46页

作者： Jeonghun Baek Chun Wai Mai Wei Meng Lim Lai Chun Wong School of Medicine International Medical UniversityKuala Lumpur 57000Malaysia Department of Pharmaceutical Chemistry School of PharmacyInternational Medical UniversityKuala Lumpur 57000Malaysia Department of Pharmaceutical Technology School of PharmacyInternational Medical UniversityKuala Lumpur 57000Malaysia

AIM:To compare the effect of vegetable oils on the uptake of lutein and zeaxanthin by adult retinal pigment epithelial(ARPE)-19 cells in ***:ARPE-19 cells were cultured in Dulbecco’s Modified Eagle Medium-F-12 supplemented with 10%foetal bovine serum and 1%penicillin–streptomycin in a humidified 5%CO_(2) incubator maintained at 37℃.Cells were treated with 247μmol/L lutein,49μmol/L zeaxanthin and 1%(v/v)of either coconut oil,corn oil,peanut oil,olive oil,sunflower oil,soybean oil,castor oil,or linseed oil for *** and zeaxanthin concentration in the cells were quantified by high performance liquid ***:Among the oils tested,the highest lutein and zeaxanthin uptake was observed with coconut oil while the lowest was observed with linseed ***:ARPE-19 uptake of lutein and zeaxanthin are found to be dependent on the type of oils.

关键词： lutein zeaxanthin adult retinal pigment epithelial-19 cells vegetable oil