Vitreoscilla hemoglobin gene(vgb)improves lutein production in Chlorella vulgaris

作     者：马瑞娟 林祥志 

作者机构：Engineering Research Center of Marine Biological Resource Comprehensive UtilizationThird Institute of OceanographyState Oceanic Administration 

出 版 物：《Chinese Journal of Oceanology and Limnology》 (中国海洋湖沼学报（英文版）)

年 卷 期：2014年第32卷第2期

页      面：390-396页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：Supported by the Ocean Public Welfare Scientific Research Special Appropriation Project(No.201005020) 

主　　题：Chlorella vulgaris Vitreoscilla hemoglobin gene (vgb) genetic engineering lutein 

摘      要：Vitreoscilla hemoglobin is an oxygen-binding protein that promotes oxygen delivery and reduces oxygen consumption under low oxygen conditions to increase the effi ciency of cell respiration and *** this study,we introduced a Vitreoscilla hemoglobin gene(vgb)into Chlorella vulgaris by Agrobacterium tumefaciens-mediated transformation(ATMT).PCR analysis confi rmed that the vgb gene was successfully integrated into the Chlorella vulgaris *** of biomass obtained in shake fl asks revealed transformant biomass concentrations as high as 3.28 g/L,which was 38.81% higher than that of the wild-type *** content of transformants also increased *** experiments recovered a maximum lutein yield of 2.91 mg/L from the transformants,which was 36.77% higher than that of the wild-type *** above results suggest that integrated expression of the vgb gene may improve cell growth and lutein yield in Chlorella vulgaris,with applications to lutein production from Chlorella during fermentation.