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Impact of low-energy ion beam Implantation on the Expression of Ty1-copia-like Retrotransposons in Wheat(Triticum aestivum)

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Plasma Science and Technology 2007年第5期9卷 626-633页

作者：押辉远焦浈谷运红王卫东秦广雍霍裕平 Henan Provincial Key Laboratory of ion beam Bioengineering Zhengzhou UniversityZhengzhou 450052China College of Life Sciences Luoyang Normal UniversityLuoyang 471022China Henan Provincial Key Laboratory of ion beam Bioengineering Zhengzhou UniversityZhengzhou 450052China

Retrotransposon-like elements are major constituents of most eukaryotic genomes. For example, they account for roughly 90% of the wheat （Triticum aestivum） genome, Previous study on a wheat strain treated by low-energy N^＋ ions indicated the variations in AFLP （Amplified Fragment Length Polymorphism ） markers, One such variation was caused by the re-activation of Tyl-copia-like retrotransposons, implying that the mutagenic effects of lowenergy ions might work through elevated activation of retrotransposons, In this paper an expression profile of Tyl-copia-like retrotransposons in wheat treated by low-energy N^＋ ions is reported, The reverse transcriptase （RT） domains of these retrotransposons were amplified by reverse-transcriptional polymerase chain reaction （RT-PCR） and sequentially cloned, 42 and 65 clones were obtained from the treated （CL） and control materials （CK）, respectively, Sequence analysis of each clone was performed by software. Phylogeny and classification were calculated responding to the sequences of the RT domains. All the results show that there is much difference in the RT domain between the control sample and the treated sample, Especially, the RT domains from the treated group encode significantly more functional ORF （open reading frames） than those from the control sample, This observation suggests that the treated sample has higher activation of retrotransposons, possibly as a consequence of low-energy ion beam irradiation, It also suggests that retrotransposons in the two groups impact the host gene expression in two different ways and carry out different functions in wheat cells.

关键词： low-energy ion beam mutation mechanism Tyl-copia-like Retrotransposons,phylogeny gene expression

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Analysis of a Partial Male-Sterile Mutant of Arabidopsis thaliana Isolated from a low-energy Argon ion beam Mutagenized Pool

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Plasma Science and Technology 2008年第2期10卷 265-269页

作者：徐敏卞坡吴跃进余增亮 Key Laboratory of ion beam Bio-engineering Institute of Plasma Physics Chinese Academy of Sciences Hefei 230031 China

A screen for Arabidopsis fertility mutants, mutagenized by low-energy argon ion beam, yielded two partial male-sterile mutants tc243-1 and tc243-2 which have similar phenotypes. tc243-2 was investigated in detail. The segregation ratio of the mutant phenotypes in the M2 pools suggested that mutation behaved as single Mendelian recessive mutations, tc243 showed a series of mutant phenotypes, among which partial male-sterile was its striking mutant characteristic. Phenotype analysis indicates that there are four factors leading to male sterility, a. Floral organs normally develop inside the closed bud, but the anther filaments do not elongate sufficiently to position the locules above the stigma at anthesis, b. The anther locules do not dehisce at the time of flower opening （although limited dehiscence occurs later）, c. Pollens of mutant plants develop into several types of pollens at the trinucleated stage. as determined by staining with DAPI （4＇,6-diamidino-2-phenylindole）. which shows a variable size. shape and number of nucleus. d. The viability of pollens is lower than that of the wild type on the germination test in vivo and vitro.

关键词： Arabidopsis thaliana Ar^+ low-energy ion beam Male-Sterile Mutant

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Changes of gene expression of E. coli induced by low-energy nitrogen ions

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Progress in Natural Science:Materials International 2004年第5期 65-68页

作者： DU Yanhua and QIU Guanying(College of Life Sciences, Wuhan University, Wuhan 430072, China)

Changes of gene expression in E. coli DH5α following 25 keV nitrogen ions irradiation are investigated. The results indicate: (1) The expression of total protein increases obviously after N+ ion irradiation; (2) 23 differential expression segments are separated from E. coli cells by using mRNA differential dispky reverse transcription-PCR (DDRT-PCR) and blot hybridization techniques, 12 of them are activated (damage-induced expression), 4 suppressed, 6 increased and only one decreased. These experimental data suggest that the changes of gene expression of E. coli induced by low-energy ion beam are remarkable and in a diverse form.

关键词： low-energy ion beam gene expression DDRT-PCR.

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