Changes of gene expression of E. coli induced by low-energy nitrogen ions

作     者：DU Yanhua and QIU Guanying(College of Life Sciences, Wuhan University, Wuhan 430072, China) 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：2004年第5期

页      面：65-68页

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Supported by National Natural Science Foundation of China (Grant Nos. 19890300  19775038) 

主　　题：low-energy ion beam gene expression DDRT-PCR. 

摘      要：Changes of gene expression in E. coli DH5α following 25 keV nitrogen ions irradiation are investigated. The results indicate: (1) The expression of total protein increases obviously after N+ ion irradiation; (2) 23 differential expression segments are separated from E. coli cells by using mRNA differential dispky reverse transcription-PCR (DDRT-PCR) and blot hybridization techniques, 12 of them are activated (damage-induced expression), 4 suppressed, 6 increased and only one decreased. These experimental data suggest that the changes of gene expression of E. coli induced by low-energy ion beam are remarkable and in a diverse form.