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Changes induced by osmotic stress in the morphology, biochemistry, physiology, anatomy and stomatal parameters of almond species(Prunus L. spp.) grown in vitro

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Journal of Forestry Research 2014年第3期25卷 523-534页

作者： Shakiba Rajabpoor Soghra Kiani Karim Sorkheh Farahnaz Tavakoli Department of Biology Payam e Noor University Department of Agriculture Payam e Noor University Department of Agronomy and Plant Breeding Faculty of Agriculture Shahid Chamran University of Ahwaz Department of Plant Breeding and Biotechnology Faculty of Agriculture Shahrekord University

We investigated the influence of different levels of osmotic stress on growth and development in selected wild almond species （eight Prunus spp.） grown in vitro. The study, while endorsing the efficacy of in vitro screening of auxiliary buds of wild almond for osmotic stress tolerance, showed species variability in its response to osmotic stress. Osmotic stress reduced growth and development of all the species. How-ever, the putative tolerant Prunus spp. showed better performance than the putative susceptible genotypes. On average there was an 80% de-crease in shoot dry weight at -1.2 MPa. Reduction in shoot weight was more common in osmotic stress-susceptible species in the section labeled‘Euamygdalus’. The tolerant Prunus species produced smaller changes in biochemical responses than the sensitive cultivars for malondialdehyde content, catalase activity, relative permeability of protoplast membranes, and net photosynthetic rate. The tolerant species maintained cell integrity better than drought sensitive species. Wild almond species in the section labeled ‘Spartioides’ （Prunus arabica （Olivier） Neikle, Prunus glauca＆amp;nbsp;（Browicz） A.E. Murray, Prunus scoparia Spach） and ‘Lycioides’ （Pru-nus lycioides Spach, Prunus reuteri Bossi. et Bushe） were best adapted to osmotic stress. Increase in chlorophyll concentration and leaf thickness under high osmotic stress can be considered as preliminary selection parameters for osmotic stress tolerance in Prunus spp. The study con-firmed the efficacy of the in vitro method for screening of large number of genotypes for osmotic stress tolerance in wild almond species.

关键词： Prunus spp. proline osmotic stress leaf character malon-dialdehyde protoplast membrane permeability

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Population Structure Analysis and Genome-Wide Association Study of Tea(Camellia sinensis(L.)Kuntze)Germplasm in Qiannan,China,Based on SLAF-Seq Technology

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Phyton-International Journal of Experimental Botany 2022年第4期91卷 791-809页

作者： Fen Zhang Weili Tian Lu Cen Litang Lv Xiaofang Zeng Yulu Chen Yichen Zhao Yan Li The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education) Institute of Agro-Bioengineering and College of Life SciencesGuizhou UniversityGuiyang550025China College of Tea Sciences Guizhou UniversityGuiyang550025China

Duyun Maojian tea is a famous tea in *** this study,the specific-locus amplified fragment(SLAF)sequencing method was used to analyze the population structure and conduct a genome-wide association study(GWAS)of 2 leaf traits of 123 tea plants in Qiannan,China.A total of 462,019 SLAF tags and 11,362,041 single-nucleotide polymorphism(SNP)loci were *** results of phylogenetic tree analysis,cluster analysis,and principal component analysis showed that 123 tea germplasms were clustered into three groups,and the heterozygosity rates for Groups I,II,and II were 0.206,0.224,and 0.34,***,tea germplasms in a production area are clustered in a group,indicating that tea germplasms in different production areas have certain genetic *** traditional Duyun Maojian tea core production areas,TS and DC-SJ,are clustered into Group I and Group II respectively,while the ZY production area is relatively independent in Group ***,based on GWAS analysis,11 candidate genes related to leaf apex and 7 candidate genes related to leaf shape were *** study clarified the genetic relationship among eight Duyun Maojian tea production areas and obtained candidate genes related to leaf apex and leaf shape *** results showed that population structure and candidate genes are an effective basis for the breeding of Duyun Maojian tea germplasm.

关键词： Duyun maojian tea plant SLAF-seq SNP genetic structure leaf character GWAS

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