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Quantitative Evaluation of the larval density of Sea Potato Acaudina *** Mitochondrial Molecular Marker

Journal of Ocean University of China 2021年第3期20卷 681-687页

作者： LI Xiang WANG Guilin LI Yunfan LIU Wei LU Chao GUO Yuchen HUANG Wen WANG Jinhui DONG Bo Laboratory for Morphogenesis&Evolution College of Marine Life SciencesOcean University of ChinaQingdao 266003China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Ningde Marine Environment Monitoring Center Ningde 352100China Key Laboratory of Marine Ecological Monitoring and Restoration Technology Shanghai 200137China East China Sea Branch Ministry of Natural ResourcesShanghai 200137China

The sea potato Acaudina *** a species of sea cucumber,belonging to the class Holothuroidea under Phylum *** becomes blooming recently in the East Sea of China,causing serious ecological ***,until now there are no molecular data for its larval identification and population genetic *** this study,we firstly screened a mtDNA fragment and demonstrated that it was the species-specific molecular marker for the identification of Acaudina *** then developed a quantitative polymerase chain reaction(PCR)method to evaluate the larval density of Acaudina *** on this molecular *** this method,we examined 116 plankton samples collected in four seasons from 13 stations along the coastal region in Fu-jian province,*** results showed that the high larval density was presented at stations 1,2,and 3,which were near a quay in the *** larval density increased from April and reached the highest value in June and July,suggesting temperature might be the main environmental factor on the effects of its population distribution and *** work provides an important molecular tool for species identification and risk evaluation of a potentially invasive species.

关键词： Acaudina spp. sea potato larval density population distribution molecular marker

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