Quantitative Evaluation of the Larval Density of Sea Potato Acaudina *** Mitochondrial Molecular Marker

作     者：LI Xiang WANG Guilin LI Yunfan LIU Wei LU Chao GUO Yuchen HUANG Wen WANG Jinhui DONG Bo LI Xiang;WANG Guilin;LI Yunfan;LIU Wei;LU Chao;GUO Yuchen;HUANG Wen;WANG Jinhui;DONG Bo

作者机构：Laboratory for Morphogenesis&EvolutionCollege of Marine Life SciencesOcean University of ChinaQingdao 266003China Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Ningde Marine Environment Monitoring CenterNingde 352100China Key Laboratory of Marine Ecological Monitoring and Restoration TechnologyShanghai 200137China East China Sea BranchMinistry of Natural ResourcesShanghai 200137China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2021年第20卷第3期

页      面：681-687页

核心收录：

学科分类：0710[理学-生物学] 0908[农学-水产] 07[理学] 0707[理学-海洋科学] 0815[工学-水利工程] 0824[工学-船舶与海洋工程] 0713[理学-生态学] 

基　　金：This work was supported by the National Key Research and Development Program of China(No.2018YFD0900705) the Key Laboratory of Integrated Marine Monitor-ing and Applied Technologies for Harmful Algal Blooms Funds(No.MATHAB201706) the Fundamental Re-search Funds for the Central Universities(No.201822016) 

主　　题：Acaudina spp. sea potato larval density population distribution molecular marker 

摘      要：The sea potato Acaudina *** a species of sea cucumber,belonging to the class Holothuroidea under Phylum *** becomes blooming recently in the East Sea of China,causing serious ecological ***,until now there are no molecular data for its larval identification and population genetic *** this study,we firstly screened a mtDNA fragment and demonstrated that it was the species-specific molecular marker for the identification of Acaudina *** then developed a quantitative polymerase chain reaction(PCR)method to evaluate the larval density of Acaudina *** on this molecular *** this method,we examined 116 plankton samples collected in four seasons from 13 stations along the coastal region in Fu-jian province,*** results showed that the high larval density was presented at stations 1,2,and 3,which were near a quay in the *** larval density increased from April and reached the highest value in June and July,suggesting temperature might be the main environmental factor on the effects of its population distribution and *** work provides an important molecular tool for species identification and risk evaluation of a potentially invasive species.