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Wide-field optical sizing of single nanoparticles with 10 nm accuracy

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Science China(Physics,Mechanics & Astronomy) 2021年第9期64卷 56-66页

作者： Yang Liu Yan Kuai Qiwen Zhan Joseph R.Lakowicz Douguo Zhang Advanced Laser Technology Laboratory of Anhui Province Department of Optics and Optical EngineeringUniversity of Science and Technology of ChinaHefei 230026China School of Optical-Electrical and Computer Engineering University of Shanghai for Science and TechnologyShanghai 200093China Center for Fluorescence Spectroscopy Department of Biochemistry and Molecular BiologyUniversity of Maryland School of MedicineBaltimore 21201USA

There is an increasing demand for new technologies to rapidly measure individual nanoparticles in situ for applications,including early-stage diagnosis of human diseases and environmental ***,we demonstrate a label-free wide-field optical microscopy capable of sizing dispersed non-luminescent dielectric nanoparticles(with diameters down to 22 nm)with 10 nm *** technique utilizes enhanced nanoparticle-perturbed scattering by surface plasmons created on a gold *** the meantime,an azimuthal rotation illumination module is installed on this microscope and a differential image processing technique is carried *** relationship between the scattering intensity and the particle size was experimentally measured with good consistency with the theoretical *** capability of precise measurement of the size of dispersed nanoparticles within a larger field of view in a label-free,non-invasive and quantitative manner may find broad applications involving single nanoparticle chemistry and physics.

关键词： plasmonic microscopy label-free imaging single nanoparticle optical sizing imaging and sensing

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High frame rate multi-resonance imaging refractometry with distributed feedback dye laser sensor

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Light(Science & Applications) 2015年第1期4卷 410-414页

作者： Christoph Vannahme Martin Dufva Anders Kristensen Technical University of Denmark Department of Micro-and NanotechnologyDTU NanotechDK-2800 Kgs.LyngbyDenmark

High frame rate and highly sensitive imaging of refractive index changes on a surface is very promising for studying the dynamics of dissolution,mixing and biological processes without the need for ***,a highly sensitive distributed feedback(DFB)dye laser sensor for high frame rate imaging refractometry without moving parts is *** dye lasers are low-cost and highly sensitive refractive index *** unique multi-wavelength DFB laser structure presented here comprises several areas with different grating *** two dimensions of space is enabled by analyzinglaser light from all areasin parallelwith an imaging *** this multi-resonance imaging refractometry method,the spatial position in one direction is identified from the horizontal,i.e.,spectral position of the multiple laser lines which is obtained from the spectrometer charged coupled device(CCD)*** orthogonal spatial position is obtained from the vertical spatial position on the spectrometer CCD array as in established spatially resolved ***,the imaging technique is demonstrated by monitoring the motion of small sucrose molecules upon dissolution of solid sucrose in *** omission of moving parts improves the robustness of the imaging system and allows a very high frame rate of up to 12 Hz.

关键词： distributed feedback laser label-free imaging laser sensor refractive index sensor

Synchronization-free all-solid-state laser system for stimulated Raman scattering microscopy

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Light(Science & Applications) 2016年第1期5卷 481-486页

作者： Tobias Steinle Vikas Kumar Moritz Floess Andy Steinmann Marco Marangoni Claudia Koch Christina Wege Giulio Cerullo Harald Giessen th Physics Institute and Research Center SCoPE University of StuttgartD-70569StuttgartGermany IFN-CNR Dipartimento di FisicaPolitecnico di MilanoPiazza Leonardo da Vinci 32I-20133MilanItaly Institute of Biomaterials and Biomolecular Systems Dpt.of Molecular Biology and Plant VirologyUniversity of StuttgartPfaffenwaldring 57D-70569StuttgartGermany

We introduce an extremely simple and highly stable system for stimulated Raman scattering(SRS)*** 8-W,450-fs Yb:KGW bulk oscillator with 41 MHz repetition rate pumps an optical parametric amplifier,which is seeded by a cw tunable external cavity diode *** output radiation is frequency doubled in a long PPLN crystal and generates 1.5-ps long narrowband pump pulses that are tunable between 760 and 820 nm with 450 mW average *** of the oscillator output is sent through an etalon and creates Stokes pulses with 100 mW average power and 1.7 ps *** demonstrate SRS microscopy at a 30-μs pixel dwell time with high chemical contrast,signal-to-noise ratio in excess of 45 and no need for balanced detection,thanks to the favorable noise properties of the bulk solid-state *** seeding intrinsically ensures low spectral *** discuss its application to chemical contrast microscopy of freshly prepared plant tissue sections at different vibrational bands.

关键词： label-free imaging stimulated Raman scattering optical parametric amplifier spectral compression

Multiplane quantitative phase imaging using a wavelength-multiplexed diffractive optical processor

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Advanced Photonics 2024年第5期6卷 55-73页

作者： Che-Yung Shen Jingxi Li Yuhang Li Tianyi Gan Langxing Bai Mona Jarrahi Aydogan Ozcan University of California Los Angeles Electrical and Computer Engineering Department University of California Los Angeles Bioengineering Department University of California Los Angeles California Nano Systems Institute University of California Los Angeles Department of Computer Science

Quantitative phase imaging(QPI) is a label-free technique that provides optical path length information for transparent specimens, finding utility in biology, materials science, and engineering. Here,we present QPI of a three-dimensional(3D) stack of phase-only objects using a wavelength-multiplexed diffractive optical processor. Utilizing multiple spatially engineered diffractive layers trained through deep learning, this diffractive processor can transform the phase distributions of multiple two-dimensional objects at various axial positions into intensity patterns, each encoded at a unique wavelength *** wavelength-multiplexed patterns are projected onto a single field of view at the output plane of the diffractive processor, enabling the capture of quantitative phase distributions of input objects located at different axial planes using an intensity-only image sensor. Based on numerical simulations, we show that our diffractive processor could simultaneously achieve all-optical QPI across several distinct axial planes at the input by scanning the illumination wavelength. A proof-of-concept experiment with a 3D-fabricated diffractive processor further validates our approach, showcasing successful imaging of two distinct phase objects at different axial positions by scanning the illumination wavelength in the terahertz *** network-based multiplane QPI designs can open up new avenues for compact on-chip phase imaging and sensing devices.

关键词： quantitative phase imaging three-dimensional imaging label-free imaging diffractive neural networks computational imaging

Multiscale label-free volumetric holographic histopathology of thick-tissue slides with subcellular resolution

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Advanced Photonics 2021年第2期3卷 98-105页

作者： Herve Hugonnet Yeon Wook Kim Moosung Lee Seungwoo Shin Ralph H.Hruban Seung-Mo Hong YongKeun Park Korea Advanced Institute of Science and Technology Department of PhysicsDaejeonRepublic of Korea KAIST Institute for Health Science and Technology KAISTDaejeonRepublic of Korea Asan Institute for Life Science Asan Medical CenterSeoulRepublic of Korea Johns Hopkins Medical Institutions Sol Goldman Pancreatic Cancer Research CenterDepartment of PathologyBaltimoreMarylandUnited States University of Ulsan College of Medicine Asan Medical CenterDepartment of PathologySeoulRepublic of Korea Tomocube Inc. DaejeonRepublic of Korea

Histopathology relies upon the staining and sectioning of biological tissues,which can be laborious and may cause artifacts and distort *** develop label-free volumetric imaging of thick-tissue slides,exploiting refractive index distributions as intrinsic imaging *** present method systematically exploits label-free quantitative phase imaging techniques,volumetric reconstruction of intrinsic refractive index distributions in tissues,and numerical algorithms for the seamless stitching of multiple three-dimensional tomograms and for reducing scattering-induced image *** demonstrated label-free volumetric imaging of thick tissues with the field of view of 2 mm×1.75 mm×0.2 mm with a spatial resolution of 170 nm×170 nm×1400 *** number of optical modes,calculated as the reconstructed volume divided by the size of the point spread function,was∼20 giga *** have also demonstrated that different tumor types and a variety of precursor lesions and pathologies can be visualized with the present method.

关键词： optical diffraction tomography histology volumetric imaging label-free imaging wide field of view

Robust and rapidly tunable light source for SRS/CARS microscopy with low-intensity noise

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Advanced Photonics 2019年第5期1卷 2-8页

作者： Heiko Linnenbank Tobias Steinle Florian Morz Moritz Flöss Han Cui Andrew Glidle Harald Giessen SI Stuttgart Instruments GmbH ÖhringenGermany University of Stuttgart 4th Physics Institute and Research Center SCoPEStuttgartGermany University of Glasgow College of Science and EngineeringDivision of Biomedical EngineeringGlasgowUnited Kingdom

We present a fully automated laser system with low-intensity noise for coherent Raman scattering *** robust two-color system is pumped by a solid-state oscillator,which provides Stokes pulses fixed at 1043 *** tunable pump pulses of 750 to 950 nm are generated by a frequency-doubled fiberfeedback femtosecond optical parametric *** resulting pulse duration of 1.2 ps provides a viable compromise between optimal coherent Raman scattering signal and the necessary spectral *** a spectral range of 1015 to 3695 cm−1 with spectral resolution of<13 cm−1 can be addressed.

关键词： label-free imaging stimulated Raman scattering optical parametric amplifier spectral compression.

High spatial and temporal resolution synthetic aperture phase microscopy

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Advanced Photonics 2020年第6期2卷 78-85页

作者： Cheng Zheng Di Jin Yanping He Hongtao Lin Juejun Hu Zahid Yaqoo Peter T.C.So Renjie Zhou The Chinese University of Hong Kong Department of Biomedical EngineeringHong KongChina Massachusetts Institute of Technology Department of Mechanical EngineeringCambridgeMassachusettsUnited States Massachusetts Institute of Technology Computer Science and Artificial Intelligence LaboratoryCambridgeMassachusettsUnited States Zhejiang University College of Information Science and Electronic EngineeringHangzhouChina Massachusetts Institute of Technology Department of Materials Science and EngineeringCambridgeMassachusettsUnited States Massachusetts Institute of Technology Laser Biomedical Research CenterCambridgeMassachusettsUnited States Massachusetts Institute of Technology Department of Biological EngineeringCambridgeMassachusettsUnited States The Chinese University of Hong Kong Shun Hing Institute of Advanced EngineeringHong KongChina

A new optical microscopy technique,termed high spatial and temporal resolution synthetic aperture phase microscopy(HISTR-SAPM),is proposed to improve the lateral resolution of wide-field coherent *** plane wave illumination,the resolution is increased by twofold to around 260 nm,while achieving millisecond-level temporal *** HISTR-SAPM,digital micromirror devices are used to actively change the sample illumination beam angle at high speed with high *** off-axis interferometer is used to measure the sample scattered complex fields,which are then processed to reconstruct high-resolution phase *** HISTR-SAPM,we are able to map the height profiles of subwavelength photonic structures and resolve the period structures that have 198 nm linewidth and 132 nm gap(i.e.,a full pitch of 330 nm).As the reconstruction averages out laser speckle noise while maintaining high temporal resolution,HISTR-SAPM further enables imaging and quantification of nanoscale dynamics of live cells,such as red blood cell membrane fluctuations and subcellular structure dynamics within nucleated *** envision that HISTR-SAPM will broadly benefit research in material science and biology.

关键词： quantitative phase microscopy label-free imaging material inspection cell dynamics observation