High spatial and temporal resolution synthetic aperture phase microscopy

作     者：Cheng Zheng Di Jin Yanping He Hongtao Lin Juejun Hu Zahid Yaqoo Peter T.C.So Renjie Zhou 

作者机构：The Chinese University of Hong KongDepartment of Biomedical EngineeringHong KongChina Massachusetts Institute of TechnologyDepartment of Mechanical EngineeringCambridgeMassachusettsUnited States Massachusetts Institute of TechnologyComputer Science and Artificial Intelligence LaboratoryCambridgeMassachusettsUnited States Zhejiang UniversityCollege of Information Science and Electronic EngineeringHangzhouChina Massachusetts Institute of TechnologyDepartment of Materials Science and EngineeringCambridgeMassachusettsUnited States Massachusetts Institute of TechnologyLaser Biomedical Research CenterCambridgeMassachusettsUnited States Massachusetts Institute of TechnologyDepartment of Biological EngineeringCambridgeMassachusettsUnited States The Chinese University of Hong KongShun Hing Institute of Advanced EngineeringHong KongChina 

出 版 物：《Advanced Photonics》 (先进光子学（英文）)

年 卷 期：2020年第2卷第6期

页      面：78-85页

核心收录：

学科分类：08[工学] 0803[工学-光学工程] 

基　　金：We acknowledge financial support from Hong Kong Innovation and Technology Fund(Nos.ITS/394/17 and ITS/098/18FP) Shun Hing Institute of Advanced Engineering(No.BME-p3-18) Croucher Innovation Awards 2019,and the U.S.National Institutes of Health(No.5P41EB015871-33) 

主　　题：quantitative phase microscopy label-free imaging material inspection cell dynamics observation 

摘      要：A new optical microscopy technique,termed high spatial and temporal resolution synthetic aperture phase microscopy(HISTR-SAPM),is proposed to improve the lateral resolution of wide-field coherent *** plane wave illumination,the resolution is increased by twofold to around 260 nm,while achieving millisecond-level temporal *** HISTR-SAPM,digital micromirror devices are used to actively change the sample illumination beam angle at high speed with high *** off-axis interferometer is used to measure the sample scattered complex fields,which are then processed to reconstruct high-resolution phase *** HISTR-SAPM,we are able to map the height profiles of subwavelength photonic structures and resolve the period structures that have 198 nm linewidth and 132 nm gap(i.e.,a full pitch of 330 nm).As the reconstruction averages out laser speckle noise while maintaining high temporal resolution,HISTR-SAPM further enables imaging and quantification of nanoscale dynamics of live cells,such as red blood cell membrane fluctuations and subcellular structure dynamics within nucleated *** envision that HISTR-SAPM will broadly benefit research in material science and biology.