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Simulation study on derivative phase extraction of typical blood cells under interference microscopy

Chinese Optics Letters 2014年第A1期12卷 54-57页

作者：徐媛媛王亚伟季颖金卫凤 School of Mechanical Engineering Jiangsu University Zhenjiang 212013 China Faculty of Science Jiangsu University Zhenjiang 212013 China

Quantitative phase imaging （QPI） of biological cells is an important developing technique. In this paper, a derivative method of phase extraction is put out under several typical blood cells＇ models, which is completed by numerically simulating the process of QPI. Furthermore, the first-order derivative of the phase is introduced to analyze the cell morphology, and a monocyte model is discussed as an example to demonstrate the method. It shows that the first-order derivative of the phase can be used as a tool for the identification of blood cells.

关键词： 100.5070 Phase retrieval 180.3170 interference microscopy 170.1530 Cell analysis

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Electromagnetic modeling of interference,confocal,and focus variation microscopy

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Advanced Photonics Nexus 2024年第1期3卷 104-116页

作者： Tobias Pahl Felix Rosenthal Johannes Breidenbach Corvin Danzglock Sebastian Hagemeier Xin Xu Marco Künne Peter Lehmann University of Kassel Faculty of Electrical Engineering and Computer ScienceMeasurement Technology GroupKasselGermany

We present a unified electromagnetic modeling of coherence scanning interferometry,confocal microscopy,and focus variation microscopy as the most common techniques for surface topography inspection with micro-and nanometer *** model aims at analyzing the instrument response and predicting systematic *** the main focus lies on the modeling of the microscopes,the light–surface interaction is considered,based on the Kirchhoff approximation extended to vectorial imaging ***,it can be replaced by rigorous methods without changing the microscope *** demonstrate that all of the measuring instruments mentioned above can be modeled using the same theory with some adaption to the respective *** validation,simulated results are confirmed by comparison with measurement results.

关键词： interference microscopy coherence scanning interferometry confocal microscopy focus variation microscopy electromagnetic modeling surface topography measurement

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Realization of palladium-based optomechanical cantilever hydrogen sensor

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Microsystems & Nanoengineering 2017年第1期3卷 367-372页

作者： Steven J.McKeown Xiaozhen Wang Xin Yu Lynford L.Goddard Photonic Systems Laboratory Department of Electrical and Computer EngineeringMicro and Nanotechnology LabUniversity of Illinois at Urbana-Champaign208 North Wright StreetMNTL 2254UrbanaIL 61801USA

Hydrogen has attracted attention as an alternative fuel source and as an energy storage ***,the flammability of hydrogen at low concentrations makes it a safety ***,gas concentration measurements are a vital safety *** we present the experimental realization of a palladium thin film cantilever optomechanical hydrogen gas *** measured the instantaneous shape of the cantilever to nanometer-level accuracy using diffraction phase ***,we were able to quantify changes in the curvature of the cantilever as a function of hydrogen concentration and observed that the sensor’s minimum detection limit was well below the 250 *** of our test *** the change in curvature versus the hydrogen curve for calibration,we accurately determined the hydrogen concentrations for a random sequence of *** addition,we calculated the change in film stress as a function of hydrogen concentration and observed a greater sensitivity at lower concentrations.

关键词： hydrogen detection imaging and sensing interference microscopy optomechanics optical sensors surface dynamics

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FEM-based modeling of microsphereenhanced interferometry

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Light(Advanced Manufacturing) 2022年第4期3卷 73-85页

作者： Tobias Pahl Lucie Hüser Sebastian Hagemeier Peter Lehmann Measurement Technology Group Faculty of Electrical Engineering and Computer ScienceUniversity of KasselWilhelmshöher Allee 7134121 KasselGermany

To improve the lateral resolution in microscopic imaging,microspheres are placed close to the object’s surface in order to support the imaging process by optical near-field *** microsphere-assisted measurements are part of various recent studies,no generally accepted explanation for the effect of microspheres *** nanojets,enhancement of the numerical aperture,whispering-gallery modes and evanescent waves are usually named reasons in context with microspheres,though none of these effects is proven to be decisive for the resolution *** present a simulation model of the complete microscopic imaging process of microsphere-enhanced interference microscopy including a rigorous treatment of the light scattering process at the surface of the *** model consideres objective lenses of high numerical aperture providing 3D conical illumination and *** enhanced resolution and magnification by the microsphere is analyzed with respect to the numerical aperture of the objective ***,we give a criterion for the achievable resolution and demonstrate that a local enhancement of the numerical aperture is the most likely reason for the resolution enhancement.

关键词： Electromagnetic modeling Microsphere-assisted microscopy interference microscopy Coherence scanning interferometry Simulation Finite element method

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