检索结果-南通市图书馆

Tilapia Head Glycolipid Alleviates indomethacin-Induced Gastric Ulcer via Regulating Oxidative Stress and Inflammation Through COX/PGE2 Signaling Pathway in Adult Rats

引用

Journal of Ocean University of China 2024年第3期23卷 743-754页

作者： WANG Bohui WU Haixing SHAO Mingyang JIANG Mengqi SU Ruiheng GAO Xia XIA Guanghua SHI Haohao SHEN Xuanri Hainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea College of Food Science and TechnologyHainan UniversityHaikou 570228China Collaborative Innovation Center of Marine Food Deep Processing Dalian Polytechnic UniversityDalian 116000China College of Food and Engineering Hainan Tropical Ocean UniversitySanya 572022China

The aim of this experiment was to investigate the ameliorative effect and molecular mechanism of tilapia head glycolipid(TH-GL)on indomethacin(IDM)-induced gastric ulcer in male Sprague Dawley(SD)*** gastric ulcer model was established by oral administration of 30mgkg^(-1) IDM after 7 days of TH-GL or omeprazole(OME)administration in *** the macroscopic gastric injury symptoms,gastric mucosa protective factor cyclooxygenase 1(COX-1),cyclooxygenase 2(COX-2),prostaglandin E_(2)(PGE_(2)),the levels of oxidative stress,and inflammatory cytokine expression levels in the rats were *** experimental results showed that multiple ulcers appeared on the gastric surface of the rats in the model *** to the model group,TH-GL significantly alleviated gastric ulcers and reduced the gastric damage index in *** addition,TH-GL significantly promoted the expression of constitutive enzyme COX-1 while inhibited the expression of inducible enzyme COX-2,and make PGE2 maintain at normal ***-GL also inhibited oxidative stress and inflammatory responses,increased superoxide dismutase(SOD)activity and glutathione(GSH)content,decreased the level of malondialdehyde(MDA)and the content of pro-inflammatory *** conclusion,these results suggested that TH-GL could maintain the expression levels of COX-1 and PGE2 while inhibit the expression of COX-2 in the gastric of rat and then prevent IDM-induced gastric ulcer,which may be related to the regulation of oxidative stress and inflammatory ***,TH-GL might be a new option for the prevention of gastric diseases induced by IDM.

关键词： tilapia head glycolipid indomethacin gastric ulcer oxidative stress inflammation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Prophylaxis of posterior capsule opacification through autophagy activation with indomethacin-eluting intraocular lens

引用

生物活性材料(英文) 2023年第5期23卷 539-550页

作者： Xiaobo Zhang Jing Wang Jingwei Xu Wen Xu Yin Zhang Chenqi Luo Shuang Ni Haijie Han Xingchao Shentu Juan Ye Jian Ji Ke Yao Eye Center The Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou310009PR China Zhejiang Provincial Key Lab of Ophthalmology The Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou310009PR China MOE Key Laboratory of Macromolecule Synthesis and Functionalization of Ministry of Education Department of Polymer Science and EngineeringZhejiang UniversityHangzhou310027PR China

Posterior capsule opacification(PCO)is the most common long-term postoperative complication of cataract surgery,leading to secondary vision *** intraocular lens(IOL)structure and appropriate pharmacological intervention,which provides physical barriers and biological inhibition,respectively,can block the migration,proliferation,and epithelial-mesenchymal transition(EMT)of lens epithelial cells(LECs)for PCO ***,a novel indomethacin-eluting IOL(INDOM-IOL)with an optimized sharper edge and a sustained drug release behavior was developed for PCO ***(INDOM),an ophthalmic non-steroidal anti-inflammatory drug(NSAID)used for postoperative ocular inflammation,was demonstrated to not only be able to suppress cell migration and down-regulate the expression of cyclooxygenase-2(COX-2)and EMT markers,including alpha-smooth muscle actin(α-SMA)and cyclin D1,but also promote the autophagy activation in ***,autophagy was also verified to be a potential therapeutic target for the down-regulation of EMT in *** novel IOL,serving as a drug delivery platform,could carry an adjustable dose of hydrophobic indomethacin with sustained drug release ability for more than 28 *** the rabbit PCO model,the indomethacin-eluting IOL showed excellent anti-inflammatory and anti-PCO *** summary,indomethacin is an effective pharmacological intervention in PCO prophylaxis,and the novel IOL we developed prevented PCO in vivo under its sustained indomethacin release property,which provided a promising approach for PCO prophylaxis in clinical application.

关键词： Posterior capsule opacification indomethacin Autophagy Drug-eluting IOLs Epithelial-mesenchymal transition

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pulmonary endothelium-targeted nanoassembly of indomethacin and superoxide dismutase relieves lung inflammation

引用

Acta Pharmaceutica Sinica B 2023年第11期13卷 4607-4620页

作者： Yi Yang Makhloufi Zoulikha Qingqing Xiao Feifei Huang Qi Jiang Xiaotong Li Zhenfeng Wu Wei He Shanghai Skin Disease Hospital Tongji University School of MedicineShanghai 200443China School of Pharmacy China Pharmaceutical UniversityNanjing 2111198China Department of Traditional Chinese Medicine Processing and Preparation Nanjing University of Chinese MedicineNanjing 210023China Key Laboratory of Modern Preparation of TCM Ministry of EducationJiangxi University of Chinese MedicineNanchang 330004China

Lung inflammation is an essential inducer of various diseases and is closely related to pulmonary-endothelium ***,we propose a pulmonary endothelium-targeted codelivery system of anti-inflammatory indomethacin(IND)and antioxidant superoxide dismutase(SOD)by assembling the biopharmaceutical SOD onto the“vector”of rod-like pure IND crystals,followed by coating with anti-ICAM-1 antibody(Ab)for targeting endothelial *** codelivery system has a 237 nm diameter in length and extremely high drug loading of 39%IND and 2.3%*** and biodistribution studies demonstrate the extended blood circulation and the strong pulmonary accumulation of the system after intravenous injection in the lipopolysaccharide(LPS)-induced inflammatory murine ***,the system allows a robust capacity to target pulmonary endothelium mostly due to the rod-shape and Ab coating *** vitro,the preparation shows the synergistic antiinflammatory and antioxidant effects in LPS-activated endothelial *** vivo,the preparation exhibits superior pharmacodynamic efficacy revealed by significantly downregulating the inflammatory/oxidative stress markers,such as TNF-a,IL-6,COX-2,and reactive oxygen species(ROS),in the *** conclusion,the codelivery system based on rod-like pure crystals could well target the pulmonary endothelium and effectively alleviate lung *** study offers a promising approach to combat pulmonary endothelium-associated diseases.

关键词： Codelivery Nanocrystals Superoxide dismutase indomethacin Pulmonary endothelium Inflammation Acute lung injury

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The combination of ciprofloxacin and indomethacin suppresses the level of inflammatory cytokines secreted by macrophages in vitro

引用

Chinese Journal of Traumatology 2022年第6期25卷 379-388页

作者： Ke Liu Jing Yu Yu Xia Lei-Ting Zhang Sui-Yan Li Jun Yan School of Life Science and Engineering Southwest Jiaotong UniversityChengdu 610031China State Key Laboratory of Trauma Burns and Combined InjuryResearch Institute of SurgeryDaping HospitalArmy Medical University(Third Military Medical University)Chongqing 400042China

Purpose:The combined use of antibiotics and anti-inflammatory medicine to manage bacterial endotoxin-induced inflammation following injuries or diseases is *** cytokine level produced by macrophages plays an important role in this treatment *** and indomethacin,two typical representatives of antibiotics and anti-inflammatory medicine,are cost-effective and has been reported to show satisfactory *** current study aims to investigate the effect of ciprofloxacin along with indomethacin on the secretion of inflammatory cytokines by macrophagesin ***:Primary murine peritoneal macrophages and RAW 264.7 cells were administrated with lipopolysaccharide(LPS)for 24 *** related optimal dose and time point of ciprofloxacin or indomethacin in response to macrophage inflammatory response inflammation were determined via macrophage secretion induced by ***,the effects of ciprofloxacin and indomethacin on the secretory functions and viability of various macrophages were determined by enzyme-linked immunosorbent assay and flow cytometry analysis,especially for the levels of interleukin(IL)-1β,IL-6,IL-10,and tumor necrosis factor(TNF)-α.The optimal dose and time course of ciprofloxacin affecting macrophage inflammatory response were determined by testing the maximum inhibitory effect of the drugs on pro-inflammatory factors at each concentration or time ***:According to the levels of cytokines secreted by various macrophages(1.2×10^(6) cells/well)after administration of 1μg/mL LPS,the optimal dose and usage timing for ciprofloxacin alone were 80μg/mL and 24 h,respectively,and the optimal dose for indomethacin alone was 10μg/*** with the LPS-stimulated group,the combination of ciprofloxacin and indomethacin reduced the levels of IL-1β(p<0.05),IL-6(p<0.05),IL-10(p<0.01),and TNF-α(p<0.01).Furthermore,there was greater stability in the reduction of inflammatory factor levels in the combination group compared with those

关键词： Ciprofloxacin indomethacin Inflammatory cytokines In vitro Macrophages

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

indomethacin but not a selective cyclooxygenase-2 inhibitor inhibits esophageal adenocarcinogenesis in rats

引用

World Journal of Gastroenterology 2012年第35期18卷 4866-4874页

作者： Paula Esquivias Antonio Morandeira Alfredo Escartín Carmelo Cebrián Sonia Santander Francisco Esteva María Asunción García-González Javier Ortego Angel Lanas Elena Piazuelo CIBER Digestive and Hepatic Deseases Service of Surgery University Hospital "Lozano Blesa" Service of Pathology University Hospital "Lozano Blesa" Department of Medicine University of Zaragoza Service of Clinical Biochemistry University Hospital "Lozano Blesa" Aragon Health Science Institute Service of Gastroenterology University Hospital "Lozano Blesa"

AIM： To evaluate the effects of indomethacin [dual cyclooxygenase （COX）-I/COX-2 inhibitor] and 3-（3,4-difluorophenyl）-4-（4-（methylsulfonyl） phenyl）- 2-（5H）-furanone （MF-tricyclic）（COX-2 selective inhibi- tor） in a rat experimental model of Barrett＇s esophagus and esophageal ***： A total of 112 surviving post-surgery rats were randomly divided into three groups： the control group （n = 48）, which did not receive any treatment; the indomethacin group （n = 32）, which were given 2 mg/kg per day of the COX-I/COX-2 inhibitor; and the MF-tricyclic group （n = 32）, which received 10 mg/kg per day of the selective COX-2 inhibitor. Randomly se- lected rats were killed either 8 wk or 16 wk after sur- gery. The timing of the deaths was in accordance with a previous study performed in our group. Only rats that were killed at the times designated by the protocol were included in the study. We then assessed the histology and prostaglandin E2 （PGE2） expression levels in the rat esophagi. An additional group of eight animals that did not undergo esophagojejunostomy were included in order to obtain normal esophageal tissue as a control. RESULTS： Compared to a control group with no treat- ment （vehicle-treated rats）, indomethacin treatment was associated with decreases in ulcerated esophageal mucosa （16% vs 35% and 14% vs 17%, 2 mo and 4 mo after surgery, respectively; P = 0.021）, length of intestinal metaplasia in continuity with anastomosis （2 4- 1.17 mm vs 2.29 ＋ 0.75 mm and 1.25 4- 0.42 mm vs 3.5 4- 1.54 mm, 2 mo and 4 mo after surgery, respec- tively; P = 0.007）, presence of intestinal metaplasia beyond anastomosis （20% vs 71.4% and 0% vs 60%, 2 mo and 4 mo after surgery, respectively; P = 0.009）, severity of dysplasia （0% vs 71.4% and 20% vs 85.7% high-grade dysplasia, 2 mo and 4 mo after surgery, re- spectively; P = 0.002）, and adenocarcinoma incidence （0% vs 57.1% and 0% vs 60%, 2 mo and 4 mo after surgery, respectively; P 〈 0.0001）. Treatment with the select

关键词： Intestinal metaplasia Esophageal adeno-carcinoma indomethacin MF-tricyclic

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

indomethacin suppresses growth of colon cancer via inhibition of angiogenesis in vivo

引用

World Journal of Gastroenterology 2005年第3期11卷 340-343页

作者： Hong-MeiWang Gui-YingZhang DepartmentofGastroenterology XiangyaHospitalofCentralSouthUniversityChangsha410078HunanProvinceChina

AIM: It has been reported that regular consumption of nonsteroidal anti-inflammatory drugs like indomethacin decreases the incidence and mortality rate of a number of gastrointestinal cancers. We aimed to explore the efficacy and possible mechanisms of indomethacin on tumor growth and tumor angiogenesis of human colon cancer xenografts in nude mice. METHODS: MTT (thiazolyl blue) assay was used to assess the effect of indomethacin on cultured human colorectal cancer cell line HCT116. HCT116 cells were inoculated subcutaneously into BALB/c-nu/nu mice. After oral administration of indomethacin, 3 mg/kg·d for 4 wk, animals were sacrificed by cervical dislocation. Immunohistochemical staining was employed to determine the microvessel density (MVD) and vascular endothelial growth factor (VEGF) expression in tumor tissues. RESULTS: indomethacin, a non-selective COX inhibitor, significantly decreased the viability of HCT116 cells in a dose-dependent manner (Pindomethacin. The tumor volume was significantly decreased in the treated group (458.89±32.07 mm3) compared to the control group (828.21±31.59 mm3) (Pindomethacin can significantly decrease the viability of cultured HCT116 cells and retard human colorectal HCT116 cell tumor growth via inhibiting tumor angiogenesis, which might be through reduction of VEGF expressio

关键词： Colon Cancer indomethacin Angiogenesis Microvessel density VEGF

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

indomethacin INHIBITS hCG AND GnRH AGONIST-INDUCED SECRETION OF PLASMINOGEN ACTIVATOR BY GRANULOSA AND THECA INTERSTITIAL CELLS OF HYPOPHYSECTOMIZED RATS

引用

Science China Chemistry 1988年第7期31卷 807-817页

作者：刘以训,CAJANDER STEPHEN Laboratory of Reproductive Biology Institute of Zoology Academia Sinica Beijing Department of Reproductive Medicine M-025 University of California San Diego La Jolla CA 92093 USA

Considerable data have shown that plasminogen activator (PA) may play an important role in the mechanism of ovulation. A recent report argued that PA is not a primary proteoIytic enzyme for follicular rupture because a dose of indomethacin that blocked ovulation did not inhibit ovarian PA content. To further clarify the specific role of PA in the ovulation, we have carefully examined the effect of indomethacin on the secretion of PA in granulosa and theca-interstitial (TI) cells following hCG or GnRH agonist administration in PMSG-primed hypophysectomized immature rats. We have also studies in vitro action of this compound on PA secretion in granulosa cells. The results indicate that indomethacin can only suppress hCG- and GnRH agonist-induced PA secretion, but not suppress the ovarian content of these enzymes.

关键词： indomethacin hCG GnRH agonist plasminogen activator ovulation.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Biotransformation of indomethacin by the fungus Cunninghamella blakesleeana

引用

Acta Pharmacologica Sinica 2006年第8期27卷 1097-1102页

作者： Peng ZHANG~2 Li-hong LIN~4 Hai-hua HUANG~3 Hai-yan XU~2 Da-fang ZHONG~(2,3,5) ~2Laboratory of Drug Metabolism and Pharmacokinetics,Shenyang Pharmaceutical University,Shenyang 110016.China ~3Shanghai Institute of Materia Medica.Chinese Academy of Sciences,Shanghai 201203,China ~4Department of Microbiology,Shenyang Pharmaceutical University,Shenyang 110016,China Laboratory of Drug Metabolism and Pharmacokinetics Shenyang Pharmaceutical University Shenyang 110016 China Shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai 201203 China Department of Microbiology Shenyang Pharmaceutical University Shenyang 110016 China

Aim:To investigate the biotransformation of indomethacin,the first of the newernonsteroidal anti-inflammatory drugs,by filamentous fungus and to compare thesimilarities between microbial transformation and mammalian metabolism ***:Five strains of Cunninghamella（C elegans AS 3.156,C elegans AS 3.2028,C blakesleeana AS 3.153,C blakesleeana AS 3.910 andC echinulata AS 3.2004）were screened for their ability to catalyze the biotransfor-mation of *** was partially metabolized by five strains ofCunninghamella,and C blakesleeana AS 3.910 was selected for *** metabolites produced by C blakesleeana AS 3.910 wereisolated using semi-preparative HPLC,and their structures were identified by acombination analysis of LC/MS~n and NMR *** three metabolites wereseparated and quantitatively assayed by liquid chromatography-ion trap ***:After 120 h of incubation with C blakesleeana AS 3.910,approximately 87.4% of indomethacin was metabolized to three metabolites:O-desmethylindomethacin（DMI,M1,67.2%）,N-deschlorobenzoylindomethacin（DBI,M2,13.3%）and O-desmethyl-N-deschlorobenzoylindomethacin（DMBI,M3,6.9%）.Three phase I metabolites of indomethacin produced by C blakesleeanaAS 3.910 were identical to those obtained in ***:C blakesleeanacould be a useful tool for generating the mammalian phase I metabolites ofindomethacin.

关键词： indomethacin microbial transformation Cunninghamella blakesleeana liquid chromatography mass spectrometry

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Irsogladine maleate suppresses indomethacin-induced elevation of proinflammatory cytokines and gastric injury in rats

引用

World Journal of Gastroenterology 2008年第30期14卷 4784-4790页

作者： Koyuki Tajima Kiyoto Kageyama Takashi Kyoi Department of Pharmacology Discovery Research LaboratoriesNippon Shinyaku Co. Ltd.

AIM： To investigate the mucosal protective effect and the mechanisms of action of the anti-ulcer drug irsogladine maleate in gastric injury induced by indomethacin in rats. METHODS： Gastric mucosal injury was induced in male Hos：Donryu rats by oral administration of indomethacin at a dose of 48 mg/kg. One hour before indomethacin treatment, animals were orally pretreated with irsogladine maleate at doses of 1 mg/kg, 3 mg/kg or 10 mg/kg. Four hours after indomethacin administration, the animals were sacrificed and their stomachs were rapidly removed and processed for the evaluation of gastric mucosal damage and the determination of the concentrations of tumor necrosis factor-α （TNF-α）, interleukin-1β （IL-1β）, IL-8 and myeloperoxidase （MPO） in mucosal tissues. RESULTS： Linear hemorrhagic mucosal lesions were observed primarily in the glandular stomach 4 h alter oral administration of indomethacin. Pretreatment with irsogladine maleate markedly reduced the number and severity of these lesions in a dose-dependent manner. The mucosal concentrations of proinflammatory cytokines （TNF-α, IL-1β, and IL-8） and MPO, which indicates the degree of mucosal infiltration by neutrophils, increased concomitantly with the occurrence of gastric injury in the indomethacintreated rats. Pretreatment with irsogladine maleate significantly decreased the levels of these inflammatory factors in gastric tissue elicited by ***： The mucosal protective effects afforded by irsogladine maleate on gastric injury induced by indomethacin are mediated by inhibition of mucosal proinflammatory cytokine production and neutrophil infiltration, leading to suppression of mucosal inflammation and subsequent tissue destruction.

关键词： Irsogladine Gastric injury indomethacin Cytokine Myeloperoxidase

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protective Effect of Holothurian Intestine Against indomethacin Induced Gastric Mucosal Damage in Rats

引用

Journal of Ocean University of China 2017年第3期16卷 547-554页

作者： LI Xiaoyu QIAO Xuejing ZHANG Cuiping GAO Hua NIU Qinghui WU Tong ZHANG Qi TIAN Zibin Department of Gastroenterology the Affiliated Hospital of Qingdao University Department of Pharmacy College of Medicine of Qingdao University

Our study aimed to investigate the protective effects of Holothurian intestines(HI) on NSAIDs-induced gastric mucosal damage and the possible mechanism. At first, 60 male Wistar rats were induced of gastric lesions with indomethacin(IDM, 30 mg kg^(-1)). The rats were pretreated for 15 consecutive days with saline, sucralfate, or HI(0.4g kg^(-1) d-1, 0.8 g kg^(-1) d^(-1) and 1.6 g kg^(-1) d^(-1)) prior to IDM treatment, followed by evaluations of macroscopic damage and microscopic features; and investigation of the levels of inflammatory cytokines, oxidative stress parameters, gastric mucosal prostaglandin E2(PGE2) and total hexosamine in tissues. The expression of COX-1 and COX-2 m RNA in the gastric tissue were determined by quantitative polymerase chain reaction(q PCR). Pathological gastric ulcer indexes, levels of pro-inflammatory cytokines(IL-1β, IL-17, TNF-α) and lipid peroxidation were significantly decreased in HI-treated groups, whereas the levels of protective factors(TGF-β, GSH, SOD activity and PGE2) were significantly elevated especially in the group with HI 1.61 g kg^(-1) d^(-1)(P < 0.05). Furthermore, the expression of COX-2 mRNA decreased significantly in HI groups(P < 0.05). The study investigates that holothurian intestines may act as a kind of marine medicine which have protective effect on IDM-induced gastric ulcer, which could be a dietary preventive agent for the prevention of gastric damage.

关键词： holothurian intestine NSAID indomethacin gastric ulcer sucralfate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：