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Production of functional sperm from in vitro-cultured premeiotic spermatogonia in a marine fish

Zoological Research 2022年第4期43卷 537-551页

作者： Hong Zhang Wan-Wan Zhang Cheng-Yu Mo Meng-Dan Dong Kun-Tong Jia Wei Liu Mei-Sheng Yi School of Marine Sciences Sun Yat-Sen UniversityGuangzhouGuangdong 510275China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) ZhuhaiGuangdong 519000China Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering GuangzhouGuangdong 510000China

In vitro production of functional gametes can revolutionize reproduction by reducing generation intervals and accelerating genetic breeding in aquaculture,especially in fish with relatively long ***,functional sperm production from in vitro-cultured spermatogonia remains a challenge in most aquaculture *** this study,we isolated and characterized premeiotic spermatogonia from marine four-eyed sleepers(Bostrychus sinensis),which are prone to ovotesticular or sterile testicular development,and induced the differentiation of the spermatogonia into flagellated sperm in a three-dimensional(3D)culture *** insemination indicated that the in vitro-derived sperm were capable of fertilizing mature oocytes to develop into normal ***,melatonin significantly promoted spermatogonia proliferation and differentiation through the ERK1/2 signaling pathway,and thus increased the efficiency in functional sperm *** 3D culture system and resulting functional sperm hold great promise for improving the genetic breeding of aquaculture fish.

关键词： in vitro spermatogenesis 3D culture model Spermatogonia Four-eyed sleeper Melatonin Genetic breeding

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精原干细胞：进展和前景

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Asian Journal of Andrology 2015年第5期17卷 771-775,I0007-I0008页

作者： Mitsuru Komeya Takehiko Ogawa Department of Urology Graduate School of Medicine Yokohama City University Yokohama 236-0004 Japan Laboratory of Proteomics Institute of Molecular Medicine and Life Science Yokohama City University Association of Medical Science Yokohama 236-0004 Japan

Twenty years ago, the transplantation of spermatogonial stem cells （SSCs） from a mouse to other recipient mice was shown to be feasible, which clearly demonstrated the functional identity of SSCs. Since then, several important new findings and other technical developments have followed, which included a new hypothesis on their cell kinetics and spermatogonial hierarchy in the testis, a culture method allowing their self-renewal and proliferation, a testis tissue organ culture method, which induced their complete differentiation up to sperm, and the in vitro induction of germ cells from embryonic stem cells and induced pluripotent stem cells. These advancements reinforced or advanced our understanding of this unique cell. Nonetheless, there are many unresolved questions in the study of spermatogonial stem cells and a long road remains until these cells can be used clinically in reproductive medicine.

关键词： in vitro spermatogenesis male infertility organ culture

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