Production of functional sperm from in vitro-cultured premeiotic spermatogonia in a marine fish

作     者：Hong Zhang Wan-Wan Zhang Cheng-Yu Mo Meng-Dan Dong Kun-Tong Jia Wei Liu Mei-Sheng Yi Hong Zhang;Wan-Wan Zhang;Cheng-Yu Mo;Meng-Dan Dong;Kun-Tong Jia;Wei Liu;Mei-Sheng Yi

作者机构：School of Marine SciencesSun Yat-Sen UniversityGuangzhouGuangdong 510275China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)ZhuhaiGuangdong 519000China Guangdong Provincial Key Laboratory of Marine Resources and Coastal EngineeringGuangzhouGuangdong 510000China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2022年第43卷第4期

页      面：537-551页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：supported by the National Key R&D Program of China(2018YFD0901205) National Natural Science Foundation of China(31771587,31970535) Guangdong Basic and Applied Basic Research Foundation(2020A1515010358) 

主　　题：In vitro spermatogenesis 3D culture model Spermatogonia Four-eyed sleeper Melatonin Genetic breeding 

摘      要：In vitro production of functional gametes can revolutionize reproduction by reducing generation intervals and accelerating genetic breeding in aquaculture,especially in fish with relatively long ***,functional sperm production from in vitro-cultured spermatogonia remains a challenge in most aquaculture *** this study,we isolated and characterized premeiotic spermatogonia from marine four-eyed sleepers(Bostrychus sinensis),which are prone to ovotesticular or sterile testicular development,and induced the differentiation of the spermatogonia into flagellated sperm in a three-dimensional(3D)culture *** insemination indicated that the in vitro-derived sperm were capable of fertilizing mature oocytes to develop into normal ***,melatonin significantly promoted spermatogonia proliferation and differentiation through the ERK1/2 signaling pathway,and thus increased the efficiency in functional sperm *** 3D culture system and resulting functional sperm hold great promise for improving the genetic breeding of aquaculture fish.