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Details determining the success in establishing a mouse orthotopic liver transplantation model

World Journal of Gastroenterology 2020年第27期26卷 3889-3898页

作者： Ting Li Zheng Hu Lei Wang Guo-Yue Lv Department of Hepatobiliary and Pancreatic surgery The First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education The First Hospital of Jilin UniversityChangchun 130061Jilin ProvinceChina National-Local Joint Engineering Laboratory of Animal Models for Human Diseases Changchun 130061Jilin ProvinceChina Department of Gastrointestinal surgery The First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina

Liver transplantation(LT)is currently the only effective treatment option for endstage liver *** importance of animal models in transplantation is widely recognized among *** of the well-characterized mouse genome and the greater diversity and availability of both genetically modified animals and research reagents,mouse orthotopic LT(MOLT)has become an ideal model for the investigation of liver biology,tissue injury,regulation of alloimmunity and tolerance induction,and the pathogenesis of specific liver ***,due to its complicated and technically demanding procedure,the model has merely been used by only a few research groups in the world for *** a new learner,training lasting at least a couple of months or even years is *** of the investigators have emphasized the importance of elaborate techniques and dedicated instruments in establishing a MOLT model,but some details are often *** nontechnical details are also significant,especially for researchers who have little experience in mouse ***,we review and summarize the crucial technical and nontechnical details in establishing the model of MOLT based on scientific articles and our experience in six aspects:animal selection,anesthesia,perioperative management,organ procurement,back-table preparation,and implantation *** aim to enable research groups to shorten the learning curve and implement the mouse LT procedure with high technical success.

关键词： Mouse orthotopic liver transplantation Animal selection Anesthesia Perioperative management Organ procurement Back-table preparation implantation surgery

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Recent Advances in IOL surgery

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眼科学报 1993年第1期9卷 19-20页

作者： P.Ranga Reddy Venkateshwara Rao Puligala Sarojini Devi Eye Hospital Hyderabad Andnra Pradesn IndiaSarojini Devi Eye Hospital Hyderabad Andnra Pradesn IndiaSarojini Devi Eye Hospital Hyderabad Andnra Pradesn India

Tremendous progress is made in recent time in the field of Ophthalmology especially after advent of IOL implantation surgery. Recent advance in anaesthesia/lOL, designs/IOL, quotings/cantering, techniques/advances in ... 详细信息

关键词： IOL implantation surgery lens

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