Details determining the success in establishing a mouse orthotopic liver transplantation model
作者机构：Department of Hepatobiliary and Pancreatic SurgeryThe First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina Key Laboratory of Organ Regeneration and Transplantation of Ministry of EducationThe First Hospital of Jilin UniversityChangchun 130061Jilin ProvinceChina National-Local Joint Engineering Laboratory of Animal Models for Human DiseasesChangchun 130061Jilin ProvinceChina Department of Gastrointestinal SurgeryThe First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2020年第26卷第27期
页 面：3889-3898页
核心收录：
学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学]
基 金：the Science and Technology Department of Jilin Province,No.20180622004JC the Finance Department of Jilin Province,No.2017F004 The First Hospital of Jilin University,No.LCPYJJ2017007
主 题：Mouse orthotopic liver transplantation Animal selection Anesthesia Perioperative management Organ procurement Back-table preparation Implantation surgery
摘 要：Liver transplantation(LT)is currently the only effective treatment option for endstage liver *** importance of animal models in transplantation is widely recognized among *** of the well-characterized mouse genome and the greater diversity and availability of both genetically modified animals and research reagents,mouse orthotopic LT(MOLT)has become an ideal model for the investigation of liver biology,tissue injury,regulation of alloimmunity and tolerance induction,and the pathogenesis of specific liver ***,due to its complicated and technically demanding procedure,the model has merely been used by only a few research groups in the world for *** a new learner,training lasting at least a couple of months or even years is *** of the investigators have emphasized the importance of elaborate techniques and dedicated instruments in establishing a MOLT model,but some details are often *** nontechnical details are also significant,especially for researchers who have little experience in mouse ***,we review and summarize the crucial technical and nontechnical details in establishing the model of MOLT based on scientific articles and our experience in six aspects:animal selection,anesthesia,perioperative management,organ procurement,back-table preparation,and implantation *** aim to enable research groups to shorten the learning curve and implement the mouse LT procedure with high technical success.
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