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Structure elucidation,immunomodulatory activity,antitumor activity and its molecular mechanism of a novel polysaccharide from Boletus reticulatus Schaeff

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Food Science and Human Wellness 2023年第2期12卷 647-661页

作者： Siyuan Su Xiang Ding Yiling Hou Binbin Liu Zhouhe Du Junfeng Liu Key Laboratory of Southwest China Wildlife Resources Conservation College of Life SciencesChina West Normal UniversityNanchong 637009China Sericultural Research Institute Sichuan Academy of Agricultural SciencesNanchong 637000China College of Environmental Science and Engineering China West Normal UniversityNanchong 637009China

A new water-soluble heteropolysaccharide with a molecular weight of 15 k Da was isolated from the fruiting bodies of Boletus reticulatus *** characterization results revealed that *** Schaeff polysaccharide(BRS-X)had a backbone of 1,6-linkedα-D-galactose and 1,2,6-linkedα-D-galactose which branches were mainly composed of a terminal 4-linkedβ-D-glucose and the ratio of D-galactose and D-glucose was 5:*** assays indicated that BRS-X displayed a strong proliferative activity in T cells and B cells and promoted the secretion of immunoglobulin G(Ig G),Ig E,Ig D and Ig *** addition,BRS-X could facilitate the proliferation and phagocytosis of RAW264.7 cells and could significantly inhibit the growth of tumors in S180-bearing *** results of transcriptome sequencing analysis illustrated that total 46 genes enriched in MAPK and total 34 genes enriched in PI3 K/Akt signaling pathways in BRS-X *** protein VEGF and VEGFR expression were significantly reduced under the treatment with *** findings provide a scientific basis for the edible and medicinal value of BRS-X.

关键词： Boletus reticulatus Schaeff Polysaccharides Structure identification immunomodulatory activity Antitumor activity

immunomodulatory activity and antioxidant effects of water soluble polysaccharides extracted from the stroma of Cordyceps kyushuensis

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Immunomodulatory activity and antioxidant effects of water s...

Isolation, chemical characterization, and immunomodulatory activity of naturally acetylated hemicelluloses from bamboo shavings

中国菌物学会2015年学术年会

作者： Jinjuan Su Guoying Zhang Zhang Ning Jianya Ling State Key Laboratory of Microbial Technology Shandong University Shandong University of Traditional Chinese Medicine School of Food Science and Technology Jiangnan University

Four polysaccharide fractions were extracted and purified from the cultured stroma of Cordyceps kyushuensis using DEAE-cellulose 23 and Sephadex G-150 column chromatography. Preliminary structural characterizations were conducted by fourier transform infrared（FTIR） and physicochemical property. The antioxidant activities were evaluated by DPPH radical method and protective effect of DNA damage. Good immunomodulatory activities of the polysaccharides were also determined in vivo and in vitro. The results showed that the polysaccharides could promote splenocyte proliferation with or without concanavalin A（ConA） and lipopolysaccharide（LPS）, strengthen peritoneal macrophages to devour neutral red and increase the production of interleukin-2（IL-2）, and tumor necrosis factor-alpha（TNF-α） in serum, which provided the theoretical basis for the further application of Cordyceps kyushuensis.

关键词： Cordyceps kyushuensis Fungal-derived polysaccharides immunomodulatory activity Antioxidant activity Lymphocyte Macrophage

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第2期18卷 138-151页

作者： Ju-qing HUANG Rui-ting QI Mei-rong PANG Cong LIU Guang-yu LI Ying ZHANG Department of Food Science and Nutrition School of Biosystems Engineering and Food Science Zhejiang University Hangzhou 310058 China School of Public Health Zhejiang University Hangzhou 310058 China

Bamboo shavings, the outer or intermediate layer of bamboo stems, are the bulk of by-products produced in bamboo processing. In this study we investigated the isolation, chemical characterization, and immunostimulatory activity in vitro of the hemicelluloses from bamboo shavings. Shavings were first pretreated by steam explosion. The optimal pretreatment was found to be steam explosion at 2.2 MPa for 1 min. Following this pretreatment, the yield of hemicelluloses reached（2.05±0.22）%（based on the dry dewaxed raw materials）, which was 5.7-fold higher than that of untreated samples. Bamboo-shavings hemicellulose（BSH） was then prepared by hot water extraction and ethanol precipitation from the steam-exploded shavings. Purification of BSH by anion-exchange chromatography of diethylaminoethanol（DEAE）-sepharose Fast Flow resulted in a neutral fraction（BSH-1, purity of 95.3%, yield of 1.06%） and an acidic fraction（BSH-2, purity of 92.5%, yield of 0.79%）. The weight-average molecular weights（Mw） of BSH-1 and BSH-2 were 12 800 and 11 300 g/mol, respectively. Chemical and structural analyses by Fourier transform infrared spectroscopy（FT-IR）, 1D（^1H and ^13C） and 2D（heteronuclear single quantum correlation（HSQC）） nuclear magnetic resonance（NMR） spectra revealed that BSH-1 was O-acetylated-arabinoxylan and BSH-2 was O-acetylated-（4-Omethylglucurono）-arabinoxylan. BSH-1 had a higher content of acetyl groups than BSH-2. For the immunomodulatory activity in vitro, BSH and BSH-2 significantly stimulated mouse splenocyte proliferation while BSH-1 had no effect; BSH, BSH-1, and BSH-2 markedly enhanced the phagocytosis activity and nitric oxide production of the murine macrophage RAW264.7 in a dose-dependent manner. Our results suggest that the water-extractable hemicelluloses from steam-exploded bamboo shavings are naturally acetylated and have immunostimulatory activity.

关键词： Bamboo shavings Steam explosion pretreatment Naturally acetylated hemicelluloses Isolation Chemical characterization immunomodulatory activity

Dihydroartemisinin beneficially regulates splenic immune cell heterogeneity through the SOD3-JNK-AP-1 axis

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Science China(Life Sciences) 2022年第8期65卷 1636-1654页

作者： Yiwei Zhang Qilong Li Ning Jiang Ziwei Su Quan Yuan Lei Lv Xiaoyu Sang Ran Chen Ying Feng Qijun Chen Key Laboratory of Livestock Infectious Diseases in Northeast China Ministry of EducationKey Laboratory of ZoonosisCollege of Animal Science and Veterinary MedicineShenyang Agricultural UniversityShenyang 110866China Research Unit for Pathogenic Mechanisms of Zoonotic Parasites Chinese Academy of Medical SciencesShenyang 110866China

The immunomodulatory potential of dihydroartemisinin(DHA) has recently been highlighted;however, the potential mechanism remains to be clarified. Single-cell RNA sequencing was explored in combination with cellular and biochemical approaches to elucidate the immunomodulatory mechanisms of DHA. In this study, we found that DHA induced both spleen enlargement and rearrangement of splenic immune cell subsets in mice. It was revealed that DHA promoted the reversible expansion of effective regulatory T cells and interferon-γ^(+)cytotoxic CD8^(+)T cells in the spleen via induction of superoxide dismutase 3(SOD3) expression and increased phosphorylation of c-Jun N-terminal kinases(JNK) and its downstream activator protein 1(AP-1) transcription factors. Further, SOD3 knockout mice were resistant to the regulatory effect of DHA. Thus, DHA,through the activation of the SOD3-JNK-AP-1 axis, beneficially regulated immune cell heterogeneity and splenic immune cell homeostasis to treat autoimmune diseases.

关键词： dihydroartemisinin immunomodulatory activity single-cell RNA sequencing AP-1 transcription factor SOD3

Single-cell transcriptome analysis reveals the regulatory effects of artesunate on splenic immune cells in polymicrobial sepsis

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Journal of Pharmaceutical Analysis 2023年第7期13卷 817-829页

作者： Jiayun Chen Xueling He Yunmeng Bai Jing Liu Yin Kwan Wong Lulin Xie Qian Zhang Piao Luo Peng Gao Liwei Gu Qiuyan Guo Guangqing Cheng Chen Wang Jigang Wang State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Artemisinin Research Centerand Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing100700China Department of Nephrology Shenzhen Key Laboratory of Kidney Diseasesand Shenzhen Clinical Research Centre for GeriatricsShenzhen People's HospitalThe First Affiliated HospitalSouthern University of Science and TechnologyShenzhenGuangdong518020China Department of Gastroenterology Shenzhen HospitalSouthern Medical UniversityShenzhenGuangdong518020China

Sepsis is characterized by a severe and life-threatening host immune response to polymicrobial infection accompanied by organ *** on the therapeutic effect and mechanism of immunomodulatory drugs on the sepsis-induced hyperinflammatory or immunosuppression states of various immune cells remain *** study aimed to investigate the protective effects and underlying mechanism of artesunate(ART)on the splenic microenvironment of cecal ligation and puncture-induced sepsis model mice using single-cell RNA sequencing(scRNA-seq)and experimental *** scRNA-seq analysis revealed that ART inhibited the activation of pro-inflammatory macrophages recruited during *** could restore neutrophils’chemotaxis and immune function in the septic *** inhibited the activation of T regulatory cells but promoted the cytotoxic function of natural killer cells during *** also promoted the differentiation and activity of splenic B cells in mice with *** results indicated that ART could alleviate the inflammatory and/or immunosuppressive states of various immune cells involved in sepsis to balance the immune homeostasis within the ***,this study provided a comprehensive investigation of the regulatory effect of ART on the splenic microenvironment in sepsis,thus contributing to the application of ART as adjunctive therapy for the clinical treatment of sepsis.

关键词： Artesunate Sepsis Single-cell RNA sequencing immunomodulatory activity

In vitro cytotoxic and toxicological activities of ethanolic extract of Kaempferia galanga Linn. and its active component,ethyl-p-methoxycinnamate, against cholangiocarcinoma

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Journal of Integrative Medicine 2020年第4期18卷 326-333页

作者： Porwornwisit Tritripmongkol Tullayakorn Plengsuriyakarn Mayuri Tarasuk Kesara Na-Bangchang Graduate Program in Bioclinical Sciences Chulabhorn International College of MedicineThammasat UniversityPathum Thani 12120Thailand Center of Excellence in Molecular Biology and Pharmacology of Malaria and Cholangiocarcinoma Chulabhorn International College of MedicineThammasat UniversityPathum Thani 12120Thailand Drug Discovery and Development Center Office of Advanced Science and TechnologyThammasat UniversityPathum Thani 12120Thailand

Objective:To evaluate the cytotoxic,apoptotic,mutagenic and immunomodulatory activities of Kaempferia galanga Linn.(KG)extract and ethyl-p-methoxycinnamate(EPMC)in ***:The present study investigated the cytotoxic[using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphe nyl-2-H-tetrazolium bromide test],apoptotic(using a mitochondrial membrane potential assay),mutagenic(using a micronucleus test)and immunomodulatory(using flow cytometry)activities of the ethanolic extract of KG and its bioactive component,EPMC,against two cholangiocarcinoma(CCA)cell lines,CL-6 and HuCCT1,and one normal human cell line,OUMS-36 T-1 ***:Both KG extract and EPMC exhibited moderate cytotoxic activity against both CCA *** cytotoxic activity was supported by their concentration-dependent induction of ***-6 was most sensitive(3–4 fold)and selective to 5-fluorouracil(5-FU),compared with KG extract and EPMC[median half inhibiting concentration(IC50)and selectivity index(SI)were 23.01 lg/mL and 17.32;78.41 lg/mL and 4.44;100.76 lg/mL and 2.20,respectively for 5-FU *** extract ***].HuCCT1 was relatively more sensitive and selective to 5-FU and EPMC than KG extract[median IC50 and SI were 66.03 lg/mL and6.04;60.90 lg/mL and 3.65;156.60 lg/mL and 2.23,respectively for 5-FU *** *** extract].EPMC produced relatively potent cytotoxic activity against polymorphonuclear cells(IC50=92.20 lg/mL).KG extract and EPMC exhibited concentration-dependent mutagenic activity,as well as inhibition of tumor necrosis factor-a and ***:Considering cytotoxic,apoptotic,immunomodulatory and mutagenic activities,further development of KG as a drug candidate is likely to focus on the oral pharmaceutical formulation of a standardized KG extract rather than isolated compounds.

关键词： Kaempferia galanga Linn. Cytotoxicity Cholangiocarcinoma Mutagenicity Apoptosis immunomodulatory activity

Effect of aqueous extract of Polygonum minus leaf on the immunity and survival of African catfish(Clarias gariepinus)

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Journal of Coastal Life Medicine 2014年第3期 209-213页

作者： Ravichandran Veerasamy Lim Shu Min Mohanraj Rita Pauline Shalini Sivadasan Christapher Varghese Harish Rajak Kasi Marimuthu Faculty of Pharmacy AIMST UniversitySemeling-08100KedahMalaysia Faculty of Pharmacy Guru Ghasidas UniversityBilaspurIndia Department of Biotechnology Faculty of Applied ScienceAIMST UniversitySemelingKedahMalaysia

Objective:To establish immunomodulatory potential of aqueous extract of Polygonum minus(***)leaves,the haematological effects and lysozyme activity of aqueous extract of *** leaf on Clarias gariepinus was ***:The fish were grouped and administered with the *** aqueous leaf extract *** and serum samples were collected from each group and examined for various blood *** turbidimetric assay for lysozyme activity using *** and the survival rate of fish against *** was carried ***:There was no significant impact(P>0.05)on white blood cell count,red blood cell count,mean corpuscular volume,mean corpuscular hemoglobin,mean corpuscular hemoglobin concentration and lysozyme activity was significantly(P<0.05)higher than *** serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase levels were significantly lower than control(P<0.05).The highest dose group showed longest ***:From this study,it is considered that the aqueous extract of *** can be used as an immunostimulant in African catfish,but continuous administration may require maintaining the protection.

关键词： Haematological effect African catfish Polygonum minus Aqueous extract Clarias gariepinus Lysozyme activity immunomodulatory activity

Extraction,characterization,antioxidant and immunomodulatory properties of the polysaccharides extracted from Cordycepskyushuensis

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Extraction,characterization,antioxidant and immunomodulatory...

中国菌物学会2016年学术年会

作者： Jinjuan Su Guoying Zhang Caiyi Li Jianya Ling State Key Laboratory of Microbial Technology Shandong University Shandong University of Traditional Chinese Medicine

Four polysaccharide fractions were extracted and purified from the cultured stroma of Cordycepskyushuensis using DEAE-cellulose23 and Sephadex G-150 column *** structuralcharacterizations were conducted by fourier transform infrared（FTIR）,NMR and physicochemical *** antioxidant activities were evaluated by DPPH radical method and protective effect of DNA *** immunomodulatory activities of thepolysaccharideswere also determined in vivoand in *** results showed that the polysaccharides could promotesplenocyteproliferation with or withoutconcanavalin A（Con A）and lipopolysaccharide（LPS）,strengthen peritoneal macrophages to devour neutral red and increase theproduction of interleukin-2（IL-2）,and tumor necrosis factor-alpha（TNF-α）in serum,which provided the theoretical basis for the further application of Cordycepskyushuensis.

关键词： Cordycepskyushuensis Fungal-derived polysaccharides immunomodulatory activity Antioxidant activity Lymphocyte Macrophage

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海胆黄多糖的免疫调节活性研究(英文)

海胆黄多糖的免疫调节活性研究(英文)