Immunomodulatory activity and antioxidant effects of water soluble polysaccharides extracted from the stroma of Cordyceps kyushuensis
作者单位：State Key Laboratory of Microbial TechnologyShandong University Shandong University of Traditional Chinese Medicine School of Food Science and TechnologyJiangnan University
会议名称：《中国菌物学会2015年学术年会》
会议日期：2015年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
关 键 词：Cordyceps kyushuensis Fungal-derived polysaccharides Immunomodulatory activity Antioxidant activity Lymphocyte Macrophage
摘 要：Four polysaccharide fractions were extracted and purified from the cultured stroma of Cordyceps kyushuensis using DEAE-cellulose 23 and Sephadex G-150 column chromatography. Preliminary structural characterizations were conducted by fourier transform infrared（FTIR） and physicochemical property. The antioxidant activities were evaluated by DPPH radical method and protective effect of DNA damage. Good immunomodulatory activities of the polysaccharides were also determined in vivo and in vitro. The results showed that the polysaccharides could promote splenocyte proliferation with or without concanavalin A（ConA） and lipopolysaccharide（LPS）, strengthen peritoneal macrophages to devour neutral red and increase the production of interleukin-2（IL-2）, and tumor necrosis factor-alpha（TNF-α） in serum, which provided the theoretical basis for the further application of Cordyceps kyushuensis.