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An Immunochromatographic Strip Test for Rapid Detection of Antibodies against Porcine Circovirus Type 2

Animal Husbandry and Feed Science 2011年第5期3卷 26-29页

作者： ZHANG Wen-tong WEI Feng WANG Jin-liang XIAO Yue-qiang SHEN Zhi-qiang Shandong Research&Development Center of Veterinary Propolis Vaccines Binzhou 256600China Shandong Lvdu Biological Technology Co. Ltd.Binzhou 256600China Shandong Binzhou Animal Science&Veterinary Medicine Academy Binzhou 256600China

Porcine cimovirus type 2 (PCV2) infection causes huge economic losses, but no serological method is available for batch detection of field samples. The aim of the study was to develop a method for large-batch detection of PCV2 infection. Colloidal gold-labeled staphylococcal protein A (SPA) was sprayed on glass fibers to prepare a conjugate pad. The recombinant ORF2 protein of PCV2 was blotted on the test line of the nitrocellulose (NC) membrane, and pig IgG was streaked on the control line of the NC membrane. Then, the immunochromatographic strip was used for detection of antibodies against PCV2. The results show that the strip test was simple and the results could be determined within 10 min with naked eyes. The test strip was highly specific for pig serum against PCV2 and no cross-reaction with pig serum against other pathogens was observed. The test strip had close similarity with ELISA. Storage at RT for 6 months did not affect the specificity and sensitivity obviously. A total of 324 clinical pig sera were detected by both ELISA and the developed test strip, and the coincidence was 98.77%. Therefore, the developed immunochromatographic strip is specific, sensitive, stable, fast and simple, and it is suitable for on-site detection of antibodies against PCV2.

关键词： Porcine circovirus type 2 immunochromatography Test strip

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Study on Rapid Detection of Tetracyclines,Fluoroquinolones and Sulfonamides in Milk

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Agricultural Biotechnology 2019年第1期8卷 241-245页

作者： Yuping WAN Xiaosheng WU Tingting CUI Dongshan CAO Zhaoqin WANG Linchen WANG Chun-yan YANG Fangyang HE Beijing Kwinbon Biotechnology Co. Ltd. Beijing Engineering Research Center of Food Safety Immunodetection Beijing Wanger Biotechnology Co. Ltd.

Aiming at the market demand for rapid detection of tetracyclines,fluoroquinolones and sulfonamides in milk,a golloidal gold immunochromatography test strip for simultaneous detection of tetracyclines,fluoroquinolones and sulfonamides in milk was prepared based on the principle of competitive inhibition immunochromatography. The performance indicators of the test strip were verified. The results showed that the test strip can simultaneously detect 4 tetracyclines,13 fluoroquinolones and 13 sulfonamides,and the detection limits all can meet the national residue limits; the tests strip exhibited false positive rate≤5% and false negative rate = 0; and no cross-reaction with other drugs was commonly found in milk,indicating good specificity. The method is simple,rapid,and has low cost and easy popularization. It provides a means for realizing on-site rapid detection and is of important practical significance to guarantee of safety of milk and dairy products in China.

关键词： Milk Tetracyclines Fluoroquinolones Sulfonamides Colloidal gold immunochromatography assay

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