An Immunochromatographic Strip Test for Rapid Detection of Antibodies against Porcine Circovirus Type 2
An Immunochromatographic Strip Test for Rapid Detection of Antibodies against Porcine Circovirus Type 2作者机构：Shandong Research&Development Center of Veterinary Propolis VaccinesBinzhou 256600China Shandong Lvdu Biological Technology Co.Ltd.Binzhou 256600China Shandong Binzhou Animal Science&Veterinary Medicine AcademyBinzhou 256600China
出 版 物：《Animal Husbandry and Feed Science》 (动物与饲料科学(英文版))
年 卷 期：2011年第3卷第5期
页 面：26-29页
学科分类：090602[农学-预防兽医学] 0905[农学-畜牧学] 09[农学] 0906[农学-兽医学]
主 题：Porcine circovirus type 2 Immunochromatography Test strip
摘 要：Porcine cimovirus type 2 (PCV2) infection causes huge economic losses, but no serological method is available for batch detection of field samples. The aim of the study was to develop a method for large-batch detection of PCV2 infection. Colloidal gold-labeled staphylococcal protein A (SPA) was sprayed on glass fibers to prepare a conjugate pad. The recombinant ORF2 protein of PCV2 was blotted on the test line of the nitrocellulose (NC) membrane, and pig IgG was streaked on the control line of the NC membrane. Then, the immunochromatographic strip was used for detection of antibodies against PCV2. The results show that the strip test was simple and the results could be determined within 10 min with naked eyes. The test strip was highly specific for pig serum against PCV2 and no cross-reaction with pig serum against other pathogens was observed. The test strip had close similarity with ELISA. Storage at RT for 6 months did not affect the specificity and sensitivity obviously. A total of 324 clinical pig sera were detected by both ELISA and the developed test strip, and the coincidence was 98.77%. Therefore, the developed immunochromatographic strip is specific, sensitive, stable, fast and simple, and it is suitable for on-site detection of antibodies against PCV2.
维普期刊数据库