检索结果-南通市图书馆

A New Method for Ultra-sensitive P24 Antigen Assay Based on Near-infrared Fluorescent Microsphere immunochromatography

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2020年第3期33卷 174-182页

作者： WANG Qi HOU Mei Ling LIU Li Peng MA Jing ZHANG Xiao Guang ZHOU Zhi Xiang CAO Yu Xi Beijing University of Technology College of Life Science and BioengineeringBeijing 100124China Beijing Runbio Biotechnology Development Co. LtdBeijing 100106China National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China

Objective To develop a rapid,highly sensitive quantitative method for detecting P24 antigen based on near-infrared fluorescent microsphere immunochromatography.Methods First,we prepared a lateral flow assay test strip,and labeled the detection antibody using a fluorescent microsphere.Second,we optimized the antibody labeling conditions.Third,we optimized the detection conditions.Fourth,we created a working curve.Fifth,we conducted a methodological assessment of the established fluorescent microsphere immunochromatography method.Sixty-six clinical samples were tested,and we compared the established fluorescent microsphere immunochromatography with the quantitative ELISA method.Results According to the working curve,the detection limit of the method is 3.4 pg/mL,and the detection range is 3.4 pg/mL to 10 ng/mL.The average intra-assay recovery was 99.6%,and the Coefficient of Variation(CV)was 5.4%–8.6%;the average inter-assay recovery was 97.3%,and the CV was 8.5%–11%.The detection rate of fluorescent microsphere immunochromatography was higher than ELISA method,and had a good correlation with ELISA.Conclusion The P24 antigen quantitative detection method based on near-infrared fluorescent microsphere immunochromatography has the advantages of rapid detection,high sensitivity,and wide detection range;thus,it is suitable for early clinical diagnosis and continuous monitoring of AIDS.

关键词： Fluorescent microsphere immunochromatography HIV P24 antigen POCT

Study on Colloidal Gold immunochromatography Assay for Rapid Detection of Spectinomycin

在线全文

学校读者我要写书评

暂无评论

Agricultural Biotechnology 2019年第1期8卷 188-189,193页

作者： Zhaoqin WANG Yuping WAN Xiaosheng WU Yu ZHANG Fangfang JIA Guangyao HAN Zhengxue PENG Fangyang HE Beijing Kwinbon Biotechnology Company Beijing Engineering Research Centre of Food Safety Immunodetection Beijing Wanger Biotechnology Company

[Objectives] This study was conducted to establish a rapid detection method for spectinomycin in pork,chicken,fish,shrimp flesh and water.[Methods]A test strip for rapid detection of spectinomycin in milk was developed by colloidal gold immunochromatography assay. [Results]The test strip had a detection limit of 50 μg/kg to milk with a detection time of 15 min,and the false positive rate and false negative rate were both 0. [Conclusions]The method is accurate,simple,reliable and convenient,and is suitable for rapid on-site spectinomycin detection.

关键词： Spectinomycin Gold immunochromatography assay rapid test strip Rapid detection

Machine Learning Approach to Enhance the Performance of MNP?Labeled Lateral Flow Immunoassay

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano-Micro Letters 2019年第1期11卷 132-146页

作者： Wenqiang Yan Kan Wang Hao Xu Xuyang Huo Qinghui Jin Daxiang Cui Department of Instrument Science and Engineering School of Electronic Information and Electrical Engineering Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment InstrumentKey Laboratory of Thin Film and Microfabrication (Ministry of Education) Shanghai Jiao Tong University School of Naval Architecture Ocean and Civil Engineering Shanghai Jiao Tong University Department of Biomedical Engineering JiLin Medical University State Key Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information Technology Chinese Academy of Sciences Faculty of Electrical Engineering and Computer Science Ningbo University

The use of magnetic nanoparticle(MNP)-labeled immunochromatography test strips(ICTSs) is very important for point-ofcare testing(POCT). However, common diagnostic methods cannot accurately analyze the weak magnetic signal from ICTSs, limiting the applications of POCT. In this study, an ultrasensitive multiplex biosensor was designed to overcome the limitations of capturing and normalization of the weak magnetic signal from MNPs on ICTSs. A machine learning model for sandwich assays was constructed and used to classify weakly positive and negative samples, which significantly enhanced the specificity and sensitivity. The potential clinical application was evaluated by detecting 50 human chorionic gonadotropin(HCG) samples and 59 myocardial infarction serum samples. The quantitative range for HCG was 1–1000 mIU mL^(-1) and the ideal detection limit was 0.014 mIU mL^(-1), which was well below the clinical threshold. Quantitative detection results of multiplex cardiac markers showed good linear correlations with standard values. The proposed multiplex assay can be readily adapted for identifying other biomolecules and also be used in other applications such as environmental monitoring, food analysis, and national security.

关键词： Point-of-care testing immunochromatography test strips Magnetic nanoparticles Machine learning Support vector machine

IMMUNOCHEMICAL IDENTIFICATION OF SPECTRINS ON THE PLASMA MEMBRANE OF LEAF CELLS OF Vicia faba

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1991年第10期36卷 862-866页

作者：王育东阎隆飞 Laboratory of Biochemistry College of Biological Sciences Beijing Agricultural University Beijing 100094 PRC

It was well established recently that a membrane skeleton was present on the plasma membrane of erythrocytes and nonerythrocytes of animals. The membrane skeleton plays an important role in the maintenance of cell shape, regulation of cell motility and stabilizing the lipid bilayer. In the previous paper we compared the membrane proteins of the plasma membrane of leaf cells of Vicia faba with the membrane skeleton pro-

关键词： spectrin membrane skeleton immunochromatography immunocytological localization.

Stool antigen tests for the management of Helicobacter pylori infection

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2013年第45期19卷 8188-8191页

作者： Tadashi Shimoyama Department of Gastroenterology Hirosaki University Graduate School of Medicine Hirosaki 036-8562Japan

Stool antigen tests(SATs)are noninvasive diagnostic modules for Helicobacter pylori(H.pylori)infection.Two types of SATs exist for the diagnosis of H.pylori infection,one based on enzyme immunoassay(EIA)and another on immunochromatography(ICA).SATs do not require expensive chemical agents or specified equipment;hence,they are less expensive compared with the urea breath test.Both European and Japanese guidelines have shown that EIA-based SATs using monoclonal antibodies are useful for primary diagnosis as well as for the assessment of eradication therapy.ICA-based tests do not require particular equipment and are therefore useful in developing countries.SATs are also useful for the diagnosis of H.pylori infection in children and post gastric surgery patients.SATs performed via EIA can assess H.pylori infection in a large number of subjects,almost as well as serology.Thus,SATs would be useful or detecting current infection in such a survey to identify and eradicate H.pylori infection.The accuracy of SATs is lower when the stool samples are unformed or watery,because H.pylorispecific antigens in the stool samples are diluted.Temperature and the interval between stool sample collection and measurement also affect the results of SATs.The choice of test kit depends on the sensitivity and specificity in each region and the circumstances of each patient.

关键词： Helicobacter pylori Stool antigen test Diagnosis Enzyme immunoassay immunochromatography

The Incidence of Rotavirus and Adenovirus Infections among Children with Diarrhea in Sulaimani Province, Iraq

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Biosciences and Medicines 2016年第1期4卷 124-131页

作者： Dilshad O. Jaff Tariq A. G. Aziz Natalie R. Smith Gillings Global Gateway^TM Gillings School of Global Public Health University of North Carolina Chapel Hill USA Deptartment of Microbiology College of Medicine Sulaimani University Sulaymaniyah Iraq Department of Biostatistics Gillings School of Global Public Health University of North Carolina Chapel Hill USA

Human rotavirus and adenovirus infections are major causes of acute outbreaks and sporadic cases of gastroenteritis, occurring primarily among children less than 5 years of age. Little is known about the epidemiology of rotavirus and enteric adenovirus infections in Sulaimani and Iraq. The aim of this study was to determine the incidence and clinical significance of rotavirus and enteric adenovirus gastroenteritis and also to determine possible risk factors for rotavirus and adenovirus gastroenteritis using new simple rapid screening test (VIKIA ROTA AND ADENO). This is a qualitative test based on the immunochromatography technique. In the study, one hundred children less than 5 years of age with acute gastroenteritis admitted to Sulaimani Paediatric Hospital were studied. Rotavirus was identified in 22% of the children, adenovirus was identified in 3% of the children, and mixed rotavirus and adenovirus was identified in 2% of the children. All positive cases were younger than 2 years of age. The findings show that rotavirus is most commonly detected. However, there were no significant associations between rotavirus and adenovirus and gender, type of feeding, geographical distribution, the source of drinking water, and the past history of admission to hospital.

关键词： Rotavirus Adenovirus Gastroenteritis immunochromatography Children

Hepatitis C Status in Karachi, Pakistan, a Five-Year Survey at Civil Hospital, Karachi, Pakistan

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Clinical Medicine 2015年第11期6卷 797-804页

作者： Ghulam Fatima Shehla Shaheen Anam Razzak Suresh Kumar M. Saeed Quraishy Shahana Urooj Kazmi Immunology and Infectious Diseases Research Laboratory University of Karachi Karachi Pakistan Civil Hospital Karachi Karachi Pakistan Department of Pharmacology Ziauddin Medical College Ziauddin University Karachi Pakistan Department of Clinical Microbiology Dada Bhoy Institute of Higher Education Karachi Pakistan

Hepatitis C virus is associated with Hepatitis C which can be acute or chronic. Acute hepatitis C is usually short term illness that can lead to chronic infection, while chronic hepatitis C is a chronic liver disease leading to cirrhosis, hepatocellular carcinoma and even death. The mode of transmission is mainly parenteral and vertical. Hepatitis C is the leading cause of morbidity and mortality and a serious public health problem, worldwide as well as in Pakistan. There is no vaccine available for hepatitis C so the only way to prevent hepatitis C is by changing behaviors which can spread the disease. Pakistan is also facing a huge burden of this disease. In Pakistan, the frequency of hepatitis C virus infection ranges from 8% - 15% in the general population with variations in different parts of the country. The general method of detecting infection with HCV is to observe the presence of antibodies to the virus. The aim of this study was to determine frequency of hepatitis C among general population and to see the increasing frequency in our part. Selection of Patients: All patients visiting outpatient clinics, Hepatitis clinic and indoor patients at Civil Hospital Karachi, Pakistan from January 1, 2010 to December 31, 2014 were screened for anti HCV. Methods: Blood samples were collected from the patients in Red Topped vaccutainers and allowed to clot, then centrifuged and serum was screened for anti HCV by immunochromatography (ICT) method. Results: A total of 156,990 patients were screened for anti-HCV, 23,511 (14.98%) patients were reported positive. During the year 2010, total 11,058 patients were screened for anti HCV, 1791 (16%) were positive, while in the year 2011 total 26,921 were screened and 3908 (14.5%) were positive, during the year 2012, total 23,321 were screened, 3036 (14%) were positive, in the year 2013 we screened total 40,483 patients 6070 (15%) were positive, while in 2014, total 55,484 patients were screened and 8584 were positive (15.5%). The most affected age group is between 21 - 40 ye

关键词： HCV Anti-HCV immunochromatography

Preliminary Study on Immuno-gold Chromatography Assay for Rapid Detection of Vibrio parahaemolyticus

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Husbandry and Feed Science 2012年第5期4卷 216-220,235页

作者： KONG Fan-de LIU Yang XU Shu-fei PENG Xiao-li WU De-feng LIN Li Xiamen Entry-Exit Inspection and Quarantine Bureau Animal Science College of Fujian Agriculture and Forestry University

[ Objective] Vibrio parahaemolyticus was chosen as the material to make rabbit anti- V. parahaemolyticus polyclonal antibody, the immuno-gold chromatography assay （IGCA） was used to develop immunogold labeling test strips for detecting V. parahaemolyticus. [Methods] Rabbit anti-V, parahaemolyticus IgG-2 was used to wrap nitrocellulose membrane detection line, goat anti-rabbit IgG was used to wrap control line, and im- muno-gold was used to label rabbit anti-V, parahaemolyticus IgG-1 to establish the immuno-gold chromatography assay （IGCA） for detection of V. parahaemolyticus. [Results] Test strip detection lines of positive results along with the control line were all red, test results could be got in only five to fifteen minutes, the minimum detectable amount of this method to V. parahaemolyticus was 3.60 x 104 cfu/ml, and cross reactions wouldnt occur when detecting common intestinal bacteria like Vibrio alginolyticus, vibrio cholerae, Vibrio damsela, Vibrio metschnikovii, Citrobacter freundii and salmonella etc. The test results were undifferentiated when the test strips were stored at 4 ~C for six months, stored at room temperature for three months or stored at 37 ~C for one month. [ Condusionl The established test strip assay was simple and rapid during operation, with high sensitivity, strong specificity and good stability, the test results were easy to be observed and judged, and this assay was very suitable for grassroots breeding department application.

关键词： Vibrio parahaemolyticus immuno-gold immunochromatography

An Immunochromatographic Strip Test for Rapid Detection of Antibodies against Porcine Circovirus Type 2

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Husbandry and Feed Science 2011年第5期3卷 26-29页

作者： ZHANG Wen-tong WEI Feng WANG Jin-liang XIAO Yue-qiang SHEN Zhi-qiang Shandong Research&Development Center of Veterinary Propolis Vaccines Binzhou 256600China Shandong Lvdu Biological Technology Co. Ltd.Binzhou 256600China Shandong Binzhou Animal Science&Veterinary Medicine Academy Binzhou 256600China

Porcine cimovirus type 2 (PCV2) infection causes huge economic losses, but no serological method is available for batch detection of field samples. The aim of the study was to develop a method for large-batch detection of PCV2 infection. Colloidal gold-labeled staphylococcal protein A (SPA) was sprayed on glass fibers to prepare a conjugate pad. The recombinant ORF2 protein of PCV2 was blotted on the test line of the nitrocellulose (NC) membrane, and pig IgG was streaked on the control line of the NC membrane. Then, the immunochromatographic strip was used for detection of antibodies against PCV2. The results show that the strip test was simple and the results could be determined within 10 min with naked eyes. The test strip was highly specific for pig serum against PCV2 and no cross-reaction with pig serum against other pathogens was observed. The test strip had close similarity with ELISA. Storage at RT for 6 months did not affect the specificity and sensitivity obviously. A total of 324 clinical pig sera were detected by both ELISA and the developed test strip, and the coincidence was 98.77%. Therefore, the developed immunochromatographic strip is specific, sensitive, stable, fast and simple, and it is suitable for on-site detection of antibodies against PCV2.

关键词： Porcine circovirus type 2 immunochromatography Test strip