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Journal of Wuhan University of Technology(Materials Science) 2015年第5期30卷 1074-1084页

作者：李贵才 WANG Caiping 杨苹 ZHOU Jie ZHU Pingchuan Jiangsu Key Laboratory of Neuroregeneration Nantong University Key Laboratory for Advanced Technologies of Materials Ministry of Education Southwest Jiaotong University Guangxi Key Laboratory for Subtropical Bio-Resource Conservation and Utilization Guangxi University

The adsorption behavior of heparin and fibronectin was studied by quartz crystal microbalance with dissipation(QCM-D), and the interaction between heparin and fibronectin was evaluated using immunochemistry and isothermal titration calorimetry(ITC) measurement. The results showed that there was competitive adsorption between heparin and fibronectin, and the preadsorption of fibronectin could prevent subsequent heparin adsorption to some extent, and the adsorbed Hep/Fn complex on the surface was in a rigid form. The bioactivity of heparin and fibronectin could be affected by the bulk concentration of each, and both heparin and fibronectin in Hep/Fn complex formed under p H 4 condition displayed larger bioactivity than that formed under p H 7 condition. Moreover, the fibronectin showed more exposed cell-binding sites at the p H value lower than physiological condition. The results of ITC further suggested that the interaction between heparin and fibronectin under p H 4 was stronger than under p H 7, and the complex was also more stable. The study brings forth the detailed interaction between heparin and fibronectin, which will be helpful for better understanding the interaction mechanism of the two biomolecules. The results may be potentially useful for the development of new generation of cardiovascular biomaterials.

关键词： Heparin fibronectin QCM-D immunochemistry isothermal titration calorimetry

Cost-effective screening using a two-antibody panel for detecting mismatch repair deficiency in sporadic colorectal cancer

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World Journal of Clinical Cases 2021年第24期9卷 6999-7008页

作者： Jong Beom Kim Young Il Kim Yong Sik Yoon Jihun Kim Seo Young Park Jong Lyul Lee Chan Wook Kim In Ja Park Seok-Byung Lim Chang Sik Yu Jin Cheon Kim Division of Colon and Rectal Surgery University of Ulsan College of MedicineAsan Medical CenterSeoul 05505South Korea Department of Pathology University of Ulsan College of MedicineAsan Medical CenterSeoul 05505South Korea Department of Clinical Epidemiology and Biostatistics University of Ulsan College of MedicineAsan Medical CenterSeoul 05505South Korea

BACKGROUND The microsatellite instability(MSI)test and immunohistochemistry(IHC)are widely used to screen DNA mismatch repair(MMR)deficiency in sporadic colorectal cancer(CRC).For IHC,a two-antibody panel of MLH1 and MSH2 or four-antibody panel of MLH1,MSH2,PMS2,and MSH6 are used.In general,MSI is known as a more accurate screening test than IHC.AIM To compare two-and four-antibody panels of IHC in terms of accuracy and cost benefit on the basis of MSI testing for detecting MMR deficiency.METHODS We retrospectively analyzed patients with CRC who underwent curative surgery between 2015 and 2017 at a tertiary referral center.Both IHC with four antibodies and MSI tests were routinely performed.The sensitivity and specificity of a fourand two types of two-antibody panels(PMS2/MSH6 and MLH1/MSH2)were compared on the basis of MSI testing for detecting MMR deficiency.RESULTS High-frequency MSI was found in 5.5%(n=193)of the patients(n=3486).The sensitivities of the four-and two types of two-antibody panels were 97.4%,92.2%,and 87.6%,respectively.The specificities of the three types of panels did not differ significantly(99.6%for the four-antibody and PMS2/MSH6 panels,99.7%for the MLH1/MSH2 panel).Based on Cohen's kappa statistic(κ),four-and twoantibody panels were in almost perfect agreement with the MSI test(κ>0.9).The costs of the MSI test and the four-and two-antibody panels of IHC were approximately$200,$160,and$80,respectively.CONCLUSION Considering the cost of the four-antibody panel IHC compared to that of the twoantibody panel IHC,a two-antibody panel of PMS2/MSH6 might be the best choice in terms of balancing cost-effectiveness and accuracy.

关键词： Adenocarcinoma DNA mismatch repair immunochemistry Monoclonal antibody Microsatellite instability Cost-effectiveness

Changes in N-methyl-D-aspartate receptor 2A subunit expression caused by binocular form deprivation and chondroitin sulfate proteoglycan degradation

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Neural Regeneration Research 2011年第6期6卷 462-466页

作者： Mingming Liu Wei Qin Hanping Xie Southwest Hospital/Southwest Eye Hospital Third Military Medical University of Chinese PLA Chongqing 400038 China

Light deprivation is known to induce a significant decrease in the percentage of N-methyi-D- aspartate receptor 2A subunit （NR2A）-expressing neurons during development. The purpose of this study was to investigate the effects of binocular form deprivation （BFD） and chondroitin sulfate proteoglycan （CSPG） degradation on NR2A expression via an immunohistochemical study, around the end of a critical developmental period. The results show that the positive staining of NR2A in the normal rat visual cortex increases gradually from postnatal 3-5 weeks （P 〈 0.05）, but the changes from 5 weeks to 7 weeks were not significant. The positive staining of NR2A following BFD in the rat visual cortex slightly increased from postnatal 3-7 weeks （P 〉 0.05）. The positive staining of NR2A in the CSPG-treated group was insignificant compared with the BFD group at the same time point from 4 weeks to 7 weeks （P 〉 0.05）. Thus, the effect of BFD on NR2A expression in the rat visual cortex was similar to that of CSPG degradation around the end of the critical developmental period.

关键词： visual cortex N-methyI-D-aspartate receptor 2A subunit visual development critical period immunochemistry

ALTERATION OF G1-CYCLINS (D1 AND E) IN TRANSITIONAL CELL CARCINOMA OF HUMAN URINARY BLADDER WITH INFECTION OF HPV-18

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Chinese Journal of Cancer Research 2002年第1期14卷 64-68页

作者：郑闪何祖根刘海涛王顺宝 Department of Pathology Cancer Institute (Hospital) Chinese Academy of Medical Sciences and Peking Union Medical College Beijing

Objective: This study was designed to investigate differential pattern of G1-cyclins (D1 and E) in transitional cell carcinoma (TCC) of human urinary bladder with or without human papillomavirus-18 (HPV-18) infection. Methods: Immunohistochemistry method was used in the detection of the expression of G1-cyclins in 57 cases of TCC (7 normal bladders as control), and HPV-18 DNA was found in 29 cases by polymerases chain reaction (PCR). Results: Cyclin D1 expression was found in 41 of 57 (71.93%) TCCs and it was reverse associated with HPV (x 2=8.21, P0.05). Cyclin E expression was found in 17 of 29 (56.82%) in HPV-18 infection group and 19 of 28 (67.86%) in non-HPV infection group. There was obvious difference in the cyclin D1 and cyclin E expression between the TCC and normal tissue (x 2=7.46, P<0.05; x 2=7.45, P<0.05, respectively). Conclusion: These data demonstrated that HPV infection altered the control of G1 cell cycle. And changes of G1 cell cycle regulatory proteins, either by interaction of cellular protein with viral oncoproteins or by changes in the cellular proteins themselves, may be critical for carcinogenesis of TCC of urinary bladder.

关键词： Bladder neoplasm HPV Cyclin immunochemistry PCR

COMPARATIVE ANALYSIS OF ISOELECTRO FOCUSING PATTERN AND IMMUNOCHEM ICAL CHARACTERS OF RuBPcase OF LEAVES BETWEEN HYBRID PLANTS OF Spinacia oleracea L.+ Nicotiana tabacum L. AND THEIR PARENT PLANTS

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Chinese Science Bulletin 1991年第11期36卷 936-941页

作者：谢明唐吴伯骥 Laboratory of Plant Cell Non-carrier Genetic Manipulation Chengdu Institute of Biology Academia Sinica Chengdu 610015 PRC

Ribulose-1, 5-bisphosphate carboxylase/oxygenase (EC 4, 1, 1, 39, abbreviation: RuBPcase)is a bifunction enzyme. Because of its properties in genetic function and structure, RuBPcase has been intensively studied. In the absence of certain effective genetic markers for identifying hybrids, the analysis of the isoelectrofocusing pattern of RuBPcase has become a standard procedure to verify the nature of the hybrid plants.

关键词： RuBPcase isoelectrofocusing RuBPcase immunochemistry spinach+ tobacco hybrid.

Fos expression　incatecholaminergic　medullary　neu-rons　induced　by　chemical　stimulation　of　stomach　projecting　to　the　paraventricular　nucleus　of　hypothalamus　in　rats

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World Journal of Gastroenterology 1997年第2期3卷 15-17页

作者： DONG Yuan Xiang, XIONG Kang Hui, RAO Zhi Ren and SHI Ji Wu

Ｆｏｓｅｘｐｒｅｓｓｉｏｎｉｎｃａｔｅｃｈｏｌａｍｉｎｅｒｇｉｃｍｅｄｕｌａｒｙｎｅｕｒｏｎｓｉｎｄｕｃｅｄｂｙｃｈｅｍｉｃａｌｓｔｉｍｕｌａｔｉｏｎｏｆｓｔｏｍａｃｈｐｒｏｊｅｃｔｉｎｇｔｏｔｈｅｐａｒａｖｅｎｔ... 详细信息

Ｆｏｓｅｘｐｒｅｓｓｉｏｎｉｎｃａｔｅｃｈｏｌａｍｉｎｅｒｇｉｃｍｅｄｕｌａｒｙｎｅｕｒｏｎｓｉｎｄｕｃｅｄｂｙｃｈｅｍｉｃａｌｓｔｉｍｕｌａｔｉｏｎｏｆｓｔｏｍａｃｈｐｒｏｊｅｃｔｉｎｇｔｏｔｈｅｐａｒａｖｅｎｔｒｉｃｕｌａｒｎｕｃｌｅｕｓｏｆｈ...

关键词： medulla oblongata paraventricular hypothalamic nucleus neurons immunochemistry

Relationship between DNA ploidy, expression of ki-67 antigen and gastric cancer metastasis

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World Journal of Gastroenterology 1999年第1期5卷 15-16页

作者： XU Lei, ZHANG Su Min, WANG Yan Ping, ZHAO Feng Kai, WU Dong Ying and XIN Yan

ＮＴＲＯＤＵＣＴＩＯＮＫｉ６７ｉｓａｍｏｕｓｅｍｏｎｏｃｌｏｎａｌａｎｔｉｂｏｄｙｗｈｉｃｈｒｅｃｏｇｎｉｚｅｓａｎｕｃｌｅａｒａｎｔｉｇｅｎｅｘｐｒｅｓｅｄｉｎａｌｐｈａｓｅｓｏｆｔｈｅｃｅｌｃｙｃｌｅｅｘｃｅ... 详细信息

ＮＴＲＯＤＵＣＴＩＯＮＫｉ６７ｉｓａｍｏｕｓｅｍｏｎｏｃｌｏｎａｌａｎｔｉｂｏｄｙｗｈｉｃｈｒｅｃｏｇｎｉｚｅｓａｎｕｃｌｅａｒａｎｔｉｇｅｎｅｘｐｒｅｓｅｄｉｎａｌｐｈａｓｅｓｏｆｔｈｅｃｅｌｃｙｃｌｅｅｘｃｅｐｔＧｏａｎｄｅａｒｌｙＧ１［１］...

关键词： Ki 67 antigen neoplasms metastasis immunochemistry DNA ploidy stomach neoplasms/pathology

Determination of Calcineurin Inhibitors in Dried Blood Spots from Kidney Transplant Recipients

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American Journal of Analytical Chemistry 2013年第10期4卷 27-35页

作者： Lars Wilhelm Martin Nitschke Markus Meier Reinhard Vonthein Jan Kramer Institute of Medical Biometry and Statistics University of Lübeck Lübeck Germany LADR GmbH Medizinisches Versorgungszentrum Dr. Kramer und Kollegen Geesthacht Germany LADR GmbH Medizinisches Versorgungszentrum Dr. Kramer und Kollegen Geesthacht Germany Medical Clinic I Transplant Center University Hospital of Schleswig-Holstein Lübeck Germany Medical Clinic I Transplant Center University Hospital of Schleswig-Holstein Lübeck Germany Medical Clinic I Transplant Center University Hospital of Schleswig-Holstein Lübeck Germany Nephrology Center Reinbek and Geesthacht Reinbek Germany

Background: Determination of cyclosporine A (CsA) and tacrolimus (Tac) in dried blood spots (DBS) could enable drug monitoring in transplanted patients without the necessity of having to take venous blood samples. Therefore, we have developed a method for quantitative determination of calcineurin inhibitors (CNI) by liquid-chromatography-tandem mass spectrometry (LCMS). Methods: In a study with 68 kidney transplant recipients (KTR, 34 CsA, 34 Tac), we tested the clinical application of LCMS monitoring in DBS in comparison to LCMS in whole blood. Results: The measuring range is proven for 27.33 to 1345 ng/ml for CsA and for 1.63 to 39.7 ng/ml for Tac. The requirements for clinical chemical analyses for precision and accuracy are complied with. Stability is documented for a period of 14 days. The study showed the following deviations from LCMS in whole blood for determination of CsA and Tac in DBS after introducing a correction factor by the haematocrit (Hct) value (CsA trough level: mean = 4.7%, ±1.96 standard deviation (SD) -52.1% to 61.4%, N = 96;CsA peak level: mean = 7.3%, ±1.96 SD -39.7% to 54.4%, N = 95;Tac trough level: mean = -0.5%, ±1.96 SD -76.4% to 75.3%, N = 88;Tac peak level: mean = 3.9%, ±1.96 SD -80.1% to 88.7%, N = 92). Conclusions: Our data show comparable results with the reference method by means of LCMS in whole blood. Therefore, DBS of KTR for determination of CNI levels could be transported on filter cards by mail to the respective laboratory resistant to breakage and the hazard of infection.

关键词： Cyclosporine A Tacrolimus LCMS DBS immunochemistry Kidney Transplant

Quantitative measurement of p53-p53 antibody interactions by quartz crystal microbalance: A modelsystem for immunochemical calibration

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Journal of Biophysical Chemistry 2012年第3期3卷 211-220页

作者： Donald H. Atha Vytas Reipa Biochemical Science Division National Institute of Standards and Technology Gaithersburg USA

We are developing methods to quantify antibody interactions that include a quartz crystal microbalance (QCM) system to measure, on a molecular basis, the interaction of p53 and anti-p53 antibodies. Previously, as a model system, we developed a measurement device consisting of p53 protein (human wild type), characterized by mass spectroscopy and immobilized at various concentrations on a glass slide. The device is designed as a control to be used with immunohistochemical (IHC) assays that incorporate commercially available anti-p53 antibodies and probes. In the current study, p53 protein is covalently immobilized on a silicon dioxide-coated quartz crystal and the resonance frequency shift is followed in-situ. The functionalized sensor is then incubated with the anti-p53 antibody, which provides a direct gravimetric measure of the antibody-antigen binding. This previously described method allows the comparison of the surface immobilized protein concentrations with estimates obtained by fluorescence measurement. The p53 functionalized QCM system offers an independent measure of surface immobilized protein concentration and is essential in development of our IHC calibration prototypes. These results provide a benchmark for comparing antibody systems that may be used in developing other molecular diagnostic assays such as immunocytochemical analysis and the detection of biomarker proteins in blood and urine.

关键词： Quartz Crystal Microbalance (QCM) Antibody Characterization p53 Immobilized Protein immunochemistry Immunohistochemistry (IHC) Immunocytochemistry

The Distribution of the 5-hydroxytryptamin(5-HT) Immunoreactive Cells in the Pancreas of Guinea-pig

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Journal of Medical Colleges of PLA(China) 1989年第3期4卷 239-242页

作者：张远强黄荫乔王文超苏慧慈 Department of Histology and Embryology Fourth Military Medical University Xi'an

Immunocytochemical localization of 5-hydroxytryptamine (5-HT) containing cells of the guinea-pig pancreas was studied by means of PAP-glucose oxidase-DAB-nickel (PAP-GDN) and PAPstaining methods on paraffin sections. In this study we demonstrated the presence of 5-HTimmunoreactive cells in the endocrine (islets) and exocrine pancreas of the guinea-ping. Cells exhib-iting 54-HT immunoreactivity were found in the center and the periphery of the pancreatic islets. The5-HT immunoreactive granules were evenly distributed throughout the cytoplasm, but was faint inthe nuclei. Cells of this type were also distributed in the exocrine pancreas. Grouped or single or single cellsoccurred intercalatedly between acinar cells, as well as in the epithlium of ducts and in the connectivetissues near the ducts or acini.

关键词： serotonin immunochemistry pancrcas guinea-pig