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In vivo tracking of human adipose-derived stem cells labeled with ferumoxytol in rats with middle cerebral artery occlusion by magnetic resonance imaging

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Neural Regeneration Research 2015年第6期10卷 909-915页

作者： Yan Yin Xiang Zhou Xin Guan Yang Liu Chang-bin Jiang Jing Liu Department of Neurology the Second Affiliated Hospital of Dalian Medical University Department of Neurology the First Affiliated Hospital of Dalian Medical University College of Life Sciences Liaoning Normal University Regenerative Medicine Center First Affiliated Hospital of Dalian Medical University

Ferumoxytol, an iron replacement product, is a new type of superparamagnetic iron oxide ap- proved by the US Food and Drug Administration. Herein, we assessed the feasibility of tracking transplanted human adipose-derived stem cells labeled with ferumoxytol in middle cerebral artery occlusion-injured rats by 3.0 T MRI in vivo. 1 × 104 human adipose-derived stem cells labeled with ferumoxytol-heparin-protamine were transplanted into the brains of rats with middle cerebral artery occlusion. Neurologic impairment was scored at 1, 7, 14, and 28 days after transplantation. T2-weighted imaging and enhanced susceptibility-weighted angiography were used to observe transplanted cells. Results of imaging tests were compared with results of Prussian blue staining. The modified neurologic impairment scores were significantly lower in rats transplanted with cells at all time points except I day post-transplantation compared with rats without transplantation. Regions with hypointense signals on T2-weighted and enhanced susceptibility-weighted angiography images corresponded with areas stained by Prussian blue, suggesting the presence of superparamagnetic iron oxide particles within the engrafted cells. Enhanced susceptibility-weighted angiography image exhibited better sensitivity and contrast in tracing ferumoxytol-heparin-protamine-labeled human adipose-derived stem ceils compared with T2-weighted imaging in routine MRI.

关键词： nerve regeneration brain injury neuroimaging ferumoxytol superparamagnetic ironoxide particles human adipose-derived stem cells middle cerebral artery occlusion intracerebralinjection magnetic resonance imaging enhanced susceptibility-weighted angiography image modifiedneurological severity scores rats Prussian blue staining neural regeneration

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Differentiation of human adipose-derived stem cells into neuron-like cells by Radix Angelicae Sinensis

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Neural Regeneration Research 2013年第35期8卷 3353-3358页

作者： Qiaozhi Wang Lile Zhou Yong Guo Guangyi Liu Jiyan Cheng Hong Yu Department of Histology and Embryology Luzhou Medical College Department of Electronic Microscopy Luzhou Medical College

human adipose tissues are an ideal source of stem cells. It is important to find inducers that can safely and effectively differentiate stem cells into functional neurons for clinical use. In this study, we investigate the use of Radix Angelicae Sinensis as an inducer of neuronal differentiation. Primary human adipose-derived stem cells were obtained from adult subcutaneous fatty tissue, then pre-induced with 10% Radix Angelicae Sinensis injection for 24 hours, and incubated in serum-free Dulbecco＇s modified Eagle＇s medium/Nutrient Mixture F-12 containing 40% Radix Angelicae Sinensis to induce its differentiation into neuron-like cells. Butylated hydroxyanisole, a common in- ducer for neuronal differentiation, was used as the control. After human adipose-derived stem cells differentiated into neuron-like cells under the induction of Radix Angelicae Sinensis for 24 hours, the positive expression of neuron-specific enolase was lower than that of the butylated hydroxyani- sole-induced group, and the expression of glial fibrillary acidic protein was negative. Alter they were induced for 48 hours, the positive expression of neuron specific enolase in human adipose-derived stem cells was significantly higher than that of the butylated hydroxyanisole-induced group. Our experimental findings indicate that Radix Angelicae Sinensis can induce human adipose-derived stem cell differentiation into neuron-like cells and produce less cytotoxicity.

关键词： neural regeneration stem cells human adipose-derived stem cells Radix Angelicae Sinensis neuron-like cells differentiation adipocytes cytotoxicity grants-supported paper neuroregeneration

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