Differentiation of human adipose-derived stem cells into neuron-like cells by Radix Angelicae Sinensis

作     者：Qiaozhi Wang Lile Zhou Yong Guo Guangyi Liu Jiyan Cheng Hong Yu 

作者机构：Department of Histology and Embryology Luzhou Medical College Department of Electronic Microscopy Luzhou Medical College 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2013年第8卷第35期

页      面：3353-3358页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：financially supported by the Science and Technology Project of Sichuan Province,No.2009JY0128 the Health Ministry of Sichuan Province in China,No.20060052 

主　　题：neural regeneration stem cells human adipose-derived stem cells Radix Angelicae Sinensis neuron-like cells differentiation adipocytes cytotoxicity grants-supported paper neuroregeneration 

摘      要：Human adipose tissues are an ideal source of stem cells. It is important to find inducers that can safely and effectively differentiate stem cells into functional neurons for clinical use. In this study, we investigate the use of Radix Angelicae Sinensis as an inducer of neuronal differentiation. Primary human adipose-derived stem cells were obtained from adult subcutaneous fatty tissue, then pre-induced with 10% Radix Angelicae Sinensis injection for 24 hours, and incubated in serum-free Dulbecco＇s modified Eagle＇s medium/Nutrient Mixture F-12 containing 40% Radix Angelicae Sinensis to induce its differentiation into neuron-like cells. Butylated hydroxyanisole, a common in- ducer for neuronal differentiation, was used as the control. After human adipose-derived stem cells differentiated into neuron-like cells under the induction of Radix Angelicae Sinensis for 24 hours, the positive expression of neuron-specific enolase was lower than that of the butylated hydroxyani- sole-induced group, and the expression of glial fibrillary acidic protein was negative. Alter they were induced for 48 hours, the positive expression of neuron specific enolase in human adipose-derived stem cells was significantly higher than that of the butylated hydroxyanisole-induced group. Our experimental findings indicate that Radix Angelicae Sinensis can induce human adipose-derived stem cell differentiation into neuron-like cells and produce less cytotoxicity.