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Effects of exogenous human leptin on heat shock protein 70 expression in MCF-7 breast cancer cells and breast carcinoma of nude mice xenograft model

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Chinese Medical Journal 2012年第4期125卷 680-686页

作者： Xue Rong-quan Gu Jun-chao Yu Wei Wang Yu Zhang Zhong-tao Ma Xue-mei Capital Med Univ Beijing Friendship Hosp Dept Gen Surg Beijing 100050 Peoples R China Capital Med Univ Beijing Tiantan Hosp Dept Gen Surg Beijing 100050 Peoples R China

Background It is important to identify the multiple sites of leptin activity in obese women with breast *** this study,we examined the effect of exogenous human leptin on heat shock protein 70 （HSP70） expression in MCF-7 human breast cancer cells and in a breast carcinoma xenograft model of nude *** We cultured MCF-7 human breast cancer cells and established nude mice bearing xenograffs of these cells,and randomly divided them into experimental and control *** experimental group was treated with human leptin,while the control group was treated with the same volume of normal saline.A real-time reverse transcriptase-polymerase chain reaction （RT-PCR） assay was developed to quantify the mRNA expression of HSP70 in the MCF-7 human breast cancer cells and in tumor *** blotting analysis was applied to quantify the protein expression of HSP70 in the MCF-7 *** staining was done to assess the positive rate of HSP70 expression in the tumor *** leptin activated HSP70 in a dose-dependent manner in vitro：leptin upregulated significantly the expression of HSP70 at mRNA and protein levels in MCF-7 human breast cancer cells （P 〈0.001）.There was no significant difference in expression of HSP70 mRNA in the implanted tumors between the leptin-treated group and the control group （P〉0.05）.Immunohistochemical staining revealed no significant difference in tumor HSP70 expression between the leptin-treated group and the control group （P〉0.05）.Conclusions A nude mouse xenograft model can be safely and efficiently treated with human leptin by subcutaneous injections around the ***70 may be target of leptin in breast *** can significantly upregulate the expression of HSP70 in a dose-dependent manner in vitro.

关键词： human leptin breast cancer xenografi model heat shock protein 70

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Regulation of estrogen receptors α and β in human breast carcinoma by exogenous leptin in nude mouse xenograft model

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Chinese Medical Journal 2010年第3期123卷 337-343页

作者： YU Wei GU Jun-chao LIU Jian-zhong WANG Shao-hong WANG Yu ZHANG Zhong-tao MA Xue-mei SONG Mao-min Department of General Surgery Beijing Friendship Hospital Capital Medical University Beijing 100050 China Department of General Surgery Beijing Tiantan Hospital Capital Medical University Beijing 100050 China

Background It is essential to clarify the interactions of hormones during the progression of human breast cancer. This study examined the effects of exogenous human leptin on estrogen receptor （ER） α and β in human breast tumor tissue in a nude mouse xenograft model. Methods We created nude mice xenografts of MCF-7 human breast cancer cells, and randomly divided them into an experimental group and a control group. The mice in experimental group were injected subcutaneously around tumors with human leptin, while the control group were injected with the same dose of normal saline. A real-time RT-PCR assay was developed to quantify the mRNA of ERα, β in the tumor tissues. Western blotting analyses were used to assess the relative quantities of the ERα , β proteins. Results leptin-treated xenografted nude mice were successfully established. The amount of ERa mRNA was significantly higher in the leptin group than in the control group （P 〈0.01）, while the amount of ERβ mRNA was significantly lower in the leptin group than in the control group （P 〈0.01）. Western blotting analyses revealed that the ERa protein level was significantly higher in the leptin group than in the control group （P 〈0.01）, while the ERβ protein level was significantly lower in the leptin group than in the control group （P 〈0.01）. Conclusions Nude mouse xenograft model can be safely and serviceably treated with human leptin by subcutaneous injections around tumor. ERα, β were both targets of leptin in breast cancer. leptin can up-regulate the expression of ERa and down-regulate the expression of the ERβ in human breast tumor.

关键词： human leptin xenografl model estrogen receptors α and β

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