Effects of exogenous human leptin on heat shock protein 70 expression in MCF-7 breast cancer cells and breast carcinoma of nude mice xenograft model

作     者：Xue Rong-quan Gu Jun-chao Yu Wei Wang Yu Zhang Zhong-tao Ma Xue-mei 

作者机构：Capital Med Univ Beijing Friendship Hosp Dept Gen Surg Beijing 100050 Peoples R China Capital Med Univ Beijing Tiantan Hosp Dept Gen Surg Beijing 100050 Peoples R China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2012年第125卷第4期

页      面：680-686页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 071007[理学-遗传学] 

基　　金：This study was supported by a grant from the National Natural Science Foundation of China (No.30772121) 

主　　题：human leptin breast cancer xenografi model heat shock protein 70 

摘      要：Background It is important to identify the multiple sites of leptin activity in obese women with breast *** this study,we examined the effect of exogenous human leptin on heat shock protein 70 （HSP70） expression in MCF-7 human breast cancer cells and in a breast carcinoma xenograft model of nude *** We cultured MCF-7 human breast cancer cells and established nude mice bearing xenograffs of these cells,and randomly divided them into experimental and control *** experimental group was treated with human leptin,while the control group was treated with the same volume of normal saline.A real-time reverse transcriptase-polymerase chain reaction （RT-PCR） assay was developed to quantify the mRNA expression of HSP70 in the MCF-7 human breast cancer cells and in tumor *** blotting analysis was applied to quantify the protein expression of HSP70 in the MCF-7 *** staining was done to assess the positive rate of HSP70 expression in the tumor *** Leptin activated HSP70 in a dose-dependent manner in vitro：leptin upregulated significantly the expression of HSP70 at mRNA and protein levels in MCF-7 human breast cancer cells （P 〈0.001）.There was no significant difference in expression of HSP70 mRNA in the implanted tumors between the leptin-treated group and the control group （P〉0.05）.Immunohistochemical staining revealed no significant difference in tumor HSP70 expression between the leptin-treated group and the control group （P〉0.05）.Conclusions A nude mouse xenograft model can be safely and efficiently treated with human leptin by subcutaneous injections around the ***70 may be target of leptin in breast *** can significantly upregulate the expression of HSP70 in a dose-dependent manner in vitro.