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The bio-active components of the Mongolian medicine Horcha-6 and therapeutic mechanism in the rat migraine model

Traditional Medicine Research 2024年第2期9卷 8-17页

作者： Ao Qier Naren Mandula Qiburi Qiburi Manda Manda Tegexi Baiyin Xilinqiqige Bao Huricha Baigued Chang-Shan Wang Temuqile Temuqile De-Zhi Yang Pharmaceutical Laboratory Inner Mongolia International Mongolian HospitalHohhot 010065China College of Mongolian Medicine Inner Mongolia Medical UniversityHohhot 010110China Medical Innovation Center for Nationalities Inner Mongolia Medical UniversityHohhot 010110China Institute of Mongolian Medicinal Chemistry School of Chemistry and Chemical EngineeringInner Mongolia UniversityHohhot 010021China School of Life Sciences Inner Mongolia UniversityHohhot 010021China

Background:The active components of Horcha-6 were identified using liquid chromatography with tandem mass spectrometry.Also,we investigated the potential mechanisms that explain why Horcha-6 may be effective in treating migraines through the use of network pharmacology and a rat migraine model.Methods:After identifying the active components of Horcha-6,the corresponding genes of the active components’target were obtained from the Universal Protein database,and a“compound-target-disease”network was constructed using Cytoscape 3.9.0 software.For the in vivo experiments,nitroglycerin was injected intraperitoneally into rats to create a migraine model.Pre-treatment with Horcha-6 was administered orally for 14 days,and rats were subjected to migraine-related behavior tests.RNA sequencing was performed to identify the gene expression regulated by Horcha-6 in the trigeminal nerve.Results:A total of 903 chemical components of Horcha-6 have been collected in the liquid chromatography with tandem mass spectrometry.We discovered 55 of the Horcha-6 bio-active components that were evaluated based on their Percent Human Oral Absorption(≥30%)and DL values(≥0.185)on the traditional Chinese medicine systems pharmacology database.The“compound-target-disease”network contained 163 intersection targets with the migraine state.Gene Ontology analysis indicated that these components significantly regulated the immune response,vascular function,oxidative stress,etc.When Kyoto Encyclopedia of Genes and Genomes enrichment analysis was performed,we observed that most of the target genes were significantly enriched in the inflammation and neuro-related signaling pathway,toll-like receptor signaling pathway,neuroactive ligand-receptor interaction,etc.These predictions were further demonstrated via in vivo animal model experiments.The RNA sequencing results showed that 41 genes were down-regulated(P<0.05)and 86 genes were up-regulated(P<0.05)in the Horcha-6 treated group compared with the untreated group.Those genes were mainly involved in neu

关键词： Horcha-6 bio-active components rat migraine model inflammation hormone metabolism neurotransmitter

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Jasmonic Acid Oxidase 2 Hydroxylates Jasmonic Acid and Represses Basal Defense and Resistance Responses against Botrytis cinerea Infection

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Molecular Plant 2017年第9期10卷 1159-1173页

作者： Ekaterina Smirnova Institut de Biologie Moléculaire des Plantes CNRS Université de Strasbourg Strasbourg France Laboratoire de Chimie Organique Synthétique Institut de Chimie Université de Strasbourg CNRS Strasbourg France

Jasmonates （JAs） orchestrate immune responses upon wound/herbivore injury or infection by necrotro- phic pathogens. Elucidation of catabolic routes has revealed new complexity in jasmonate metabolism. Two integrated pathways attenuate signaling by turning over the active hormone jasmonoyl-isoleucine （JA-Ile） through w-oxidation or deconjugation, and define an indirect route forming the derivative 12OH-JA. Here, we provide evidence for a second 12OH-JA formation pathway by direct jasmonic acid （JA） oxidation. Three jasmonic acid oxidases （JAOs） of the 2-oxoglutarate dioxygenase family catalyze spe- cific oxidation of JA to 12OH-JA, and their genes are induced by wounding or infection by the fungus Botrytis cinerea. JA02 exhibits the highest basal expression, and its deficiency in jao2 mutants strongly enhanced antifungal resistance. The resistance phenotype resulted from constitutive expression of antimi- crobial markers rather than from their higher induction in infected jao2 plants and could be reversed by ectopic expression of any of the three JAOs injao2. Elevated defense injao2 was dependent on the activity of JASMONATE RESPONSE 1 （JAR1） and CORONATINE-INSENSITIVE 1 （COI1） but was not correlated with erihanced JA-Ile accumulation. Instead, jao2 mutant lines displayed altered accumulation of several JA species in healthy and challenged plants, suggesting elevated metabolic flux through JA-Ile. Collectively, these data identify the missing enzymes hydroxylating JA and uncover an important metabolic diversion mechanism for repressing basal JA defense responses.

关键词： 2-oxoglutarate oxygenase jasmonic acid defense regulation hormone metabolism Botrytis

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Expression Analysis of Genes Related to Auxin metabolism at Different Growth Stages of Pak Choi

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Horticultural Plant Journal 2020年第1期6卷 25-33页

作者： Yuxiang Hou Xueting Wang Zhujun Zhu Mengxia Sun Meilan Li Leiping Hou Collaborative Innovation Center for Improving Quality and Increasing Profits of Protected Vegetables College of HorticultureShanxi Agricultural UniversityTaiguShanxi 030801China Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province/College of Agricultural and Food Science Zhejiang A&F UniversityHangzhou 311300China

Pak choi is a low-temperature vernalized plant that readily undergoes premature bolting during spring, but little is known about the governing molecular regulation of vernalization. In order to enhance our understanding of mechanism about premature bolting, we discussed the relationship between auxin(indole-3-acetic acid, IAA) and flowering of pak choi. During vernalization, hormone metabolism is an important regulatory pathway, and IAA plays a specific role. IAA metabolism has been studied in Arabidopsis thaliana and other plants, but not in pak choi. In this paper, the IAA content in pak choi shoot apices during vernalization and different growth stages was compared. The IAA content decreased significantly after low-temperature treatment(4 °C) and then increased rapidly during vegetative growth. During floral bud initiation,the IAA content decreased rapidly and was the lowest. Expressions of genes encoding key IAA metabolic enzymes were analyzed, and a major synthetase-encoding gene was downregulated, while a key degrading enzyme-encoding gene was upregulated during each comparison period,resulting in decreased IAA content. Expressions of four genes(Bra034975, Bra030246, Bra012239 and Bra040296) were consistent with changes in the IAA content. The functions of differentially expressed genes(DEGs) were analyzed, and 15 DEGs were found to be related to IAA metabolism.The findings illuminated the molecular mechanism regulating IAA content during vernalization in pak choi.

关键词： Pak choi Brassica rapa Vernalization Auxins Gene expression hormone metabolism

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