Expression Analysis of Genes Related to Auxin Metabolism at Different Growth Stages of Pak Choi

作     者：Yuxiang Hou Xueting Wang Zhujun Zhu Mengxia Sun Meilan Li Leiping Hou Yuxiang Hou;Xueting Wang;Zhujun Zhu;Mengxia Sun;Meilan Li;Leiping Hou

作者机构：Collaborative Innovation Center for Improving Quality and Increasing Profits of Protected VegetablesCollege of HorticultureShanxi Agricultural UniversityTaiguShanxi 030801China Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province/College of Agricultural and Food ScienceZhejiang A&F UniversityHangzhou 311300China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2020年第6卷第1期

页      面：25-33页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0807[工学-动力工程及工程热物理] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 0713[理学-生态学] 090202[农学-蔬菜学] 

基　　金：supported by Shanxi Province Key Research and Development Program Key Projects (Grant No. 201703D211006 201703D211001-04-01) the Natural Science Foundation of Shanxi Province (Grant No. 201701D121101) 

主　　题：Pak choi Brassica rapa Vernalization Auxins Gene expression Hormone metabolism 

摘      要：Pak choi is a low-temperature vernalized plant that readily undergoes premature bolting during spring, but little is known about the governing molecular regulation of vernalization. In order to enhance our understanding of mechanism about premature bolting, we discussed the relationship between auxin(indole-3-acetic acid, IAA) and flowering of pak choi. During vernalization, hormone metabolism is an important regulatory pathway, and IAA plays a specific role. IAA metabolism has been studied in Arabidopsis thaliana and other plants, but not in pak choi. In this paper, the IAA content in pak choi shoot apices during vernalization and different growth stages was compared. The IAA content decreased significantly after low-temperature treatment(4 °C) and then increased rapidly during vegetative growth. During floral bud initiation,the IAA content decreased rapidly and was the lowest. Expressions of genes encoding key IAA metabolic enzymes were analyzed, and a major synthetase-encoding gene was downregulated, while a key degrading enzyme-encoding gene was upregulated during each comparison period,resulting in decreased IAA content. Expressions of four genes(Bra034975, Bra030246, Bra012239 and Bra040296) were consistent with changes in the IAA content. The functions of differentially expressed genes(DEGs) were analyzed, and 15 DEGs were found to be related to IAA *** findings illuminated the molecular mechanism regulating IAA content during vernalization in pak choi.